The expression of Bcl-2 by proliferating cells varies in different categories of B-cell lymphoma

Noraidah Masir, Margaret Jones, Abigail M. Lee, Lindsey K. Goff, Andrew J. Clear, Andrew Lister, Teresa Marafioti, David Y. Mason

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Aims: To investigate the relationship between Bcl-2 protein expression and cell proliferation at single-cell level in B-cell lymphomas using double-labelling techniques. Methods and results: The relationship between Bcl-2 protein expression and cell proliferation was explored in 124 cases of B-cell lymphoma using double immunofluorescence labelling for Bcl-2 and Ki67. In follicular lymphoma, marginal zone lymphoma and a subset of chronic lymphocytic leukaemia/small lymphocytic lymphoma (CLL/SLL), neoplastic cells tended to lose Bcl-2 when they are in cell cycle. This pattern is usually maintained in both follicular lymphoma and CLL/SLL when they undergo high-grade transformation. In mantle cell lymphoma, diffuse large B-cell lymphoma and a subset of CLL/SLL, the inverse relationship (between Bcl-2 and Ki67) was not observed, i.e. the proliferating cells tended to show co-expression of Bcl-2. Conclusions: In low-grade lymphomas, including those that are transformed, Bcl-2 expression is lost when cell proliferate. However, in more aggressive tumours (i.e. mantle cell and de novo diffuse large B-cell lymphomas) the inverse Bcl-2/Ki67 relationship was not observed. It would be of interest to explore the clinical implications in lymphoma of the presence and absence of the inverse Bcl-2/Ki67 pattern.

Original languageEnglish
Pages (from-to)617-626
Number of pages10
JournalHistopathology
Volume56
Issue number5
DOIs
Publication statusPublished - Apr 2010

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B-Cell Lymphoma
B-Cell Chronic Lymphocytic Leukemia
Follicular Lymphoma
Lymphoma, Large B-Cell, Diffuse
Lymphoma
Cell Proliferation
Mantle-Cell Lymphoma
Non-Hodgkin's Lymphoma
Fluorescent Antibody Technique
Cell Cycle
Proteins
Neoplasms

Keywords

  • B-cell lymphomas
  • Bcl-2
  • Cell proliferation
  • Double immunofluorescence

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine

Cite this

The expression of Bcl-2 by proliferating cells varies in different categories of B-cell lymphoma. / Masir, Noraidah; Jones, Margaret; Lee, Abigail M.; Goff, Lindsey K.; Clear, Andrew J.; Lister, Andrew; Marafioti, Teresa; Mason, David Y.

In: Histopathology, Vol. 56, No. 5, 04.2010, p. 617-626.

Research output: Contribution to journalArticle

Masir, N, Jones, M, Lee, AM, Goff, LK, Clear, AJ, Lister, A, Marafioti, T & Mason, DY 2010, 'The expression of Bcl-2 by proliferating cells varies in different categories of B-cell lymphoma', Histopathology, vol. 56, no. 5, pp. 617-626. https://doi.org/10.1111/j.1365-2559.2010.03524.x
Masir, Noraidah ; Jones, Margaret ; Lee, Abigail M. ; Goff, Lindsey K. ; Clear, Andrew J. ; Lister, Andrew ; Marafioti, Teresa ; Mason, David Y. / The expression of Bcl-2 by proliferating cells varies in different categories of B-cell lymphoma. In: Histopathology. 2010 ; Vol. 56, No. 5. pp. 617-626.
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