The expansion potential of human nasal septum chondrocytes for the formation of engineered cartilage

Ruszymah Idrus, Chua Kien Hui, Farah Wahida Ibrahim, Fuzina Nor Hussein, Aminuddin Bin Saim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Recent development in tissue engineering has enabled a new approach in tissue repair and reconstructive surgery using patient's own cells. Human nasal septum cartilage is an optimum candidate as a starting material for this purpose. It is essential to understand the basic requirements for monolayer culture expansion of human nasal septum chondrocytes before using it in tissue engineering. The objective of this study was to investigate the effects of different media; Ham's F12 Medium, Dulbecco's Modified Eagle Medium (DMEM) or the equal combination of both media (F12: DMEM) on monolayer culture expansion of human nasal septum chondrocytes. A hydrogel, Pluronic F-127, was used as a biomaterial for cartilage reconstruction in nude mice with culture expanded chondrocytes. We demonstrated that F12:DMEM in 1:1 ratio promoted the highest human nasal septum chondrocytes proliferation and provided sufficient numbers of chondrocytes for cartilage engineering. This medium was effective for culture expansion to more than 2000 folds within 8 weeks. Although none of the medium maintained the chondrocytes' phenotype in long-term culture, the dedifferentiated cultured chondrocytes re-express collagen type II gene after being reconstructed into engineered tissue with Pluronic F-127. This hydrogel provided a 3-dimensional environment for cultured chondrocytes to regain their chondrocytic phenotype and thus initiate the generation of a good quality engineered cartilage.

Original languageEnglish
Pages (from-to)145-152
Number of pages8
JournalScienceAsia
Volume33
Issue number2
DOIs
Publication statusPublished - Jun 2007

Fingerprint

Nasal Septum
Chondrocytes
Cartilage
Eagles
Poloxamer
Hydrogel
Tissue Engineering
Reconstructive Surgical Procedures
Nasal Cartilages
Phenotype
Collagen Type II
Biocompatible Materials
Nude Mice

Keywords

  • Cartilage
  • Human chondrocyte
  • Hydrogel
  • Tissue engineering

ASJC Scopus subject areas

  • General

Cite this

The expansion potential of human nasal septum chondrocytes for the formation of engineered cartilage. / Idrus, Ruszymah; Kien Hui, Chua; Ibrahim, Farah Wahida; Hussein, Fuzina Nor; Saim, Aminuddin Bin.

In: ScienceAsia, Vol. 33, No. 2, 06.2007, p. 145-152.

Research output: Contribution to journalArticle

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