The evolution of two-dimensional gel electrophoresis - from proteomics to emerging alternative applications

Pey Yee Lee, Neda Saraygord-Afshari, Teck Yew Low

Research output: Contribution to journalArticle

Abstract

Two-dimensional gel electrophoresis (2-DE) is a technique that has been widely applied in a variety of proteomics studies. It is capable of resolving complex protein mixtures into individual protein spots based on their isoelectric point and molecular weight, enabling large-scale analysis of protein expression patterns for deciphering their changes in different biological conditions. 2-DE is a powerful tool that empowers researchers to perform differential qualitative and quantitative proteome analysis and is particularly advantageous for characterizing protein isoforms and post-translationally modified proteins. Despite its popularity as the workhorse for proteomics in the past few decades, it has been gradually displaced by the more sophisticated and high-performance mass spectrometry-based methods. However, there are several variations of the 2-DE technique that have emerged as promising approaches that shine new light on specific niches that 2-DE could still contribute. In this review, we first provide an overview of the applications of 2-DE, its merits and pitfalls in the current proteomic research arena, followed by a discussion on several alternative approaches for potential future applications.

Original languageEnglish
Article number460763
JournalJournal of Chromatography A
DOIs
Publication statusAccepted/In press - 1 Jan 2019

Fingerprint

Electrophoresis, Gel, Two-Dimensional
Electrophoresis
Proteomics
Gels
Proteins
Isoelectric Point
Proteome
Complex Mixtures
Mass spectrometry
Mass Spectrometry
Protein Isoforms
Molecular Weight
Molecular weight
Research Personnel
Research

Keywords

  • Blue native polyacrylamide gel electrophoresis
  • Immobilized metal affinity electrophoresis
  • Serological proteome analysis
  • Two-dimensional gel electrophoresis
  • Zymography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

The evolution of two-dimensional gel electrophoresis - from proteomics to emerging alternative applications. / Lee, Pey Yee; Saraygord-Afshari, Neda; Low, Teck Yew.

In: Journal of Chromatography A, 01.01.2019.

Research output: Contribution to journalArticle

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