The effects of temperature, pH and carbon sources on antimicrobial peptide AN5-1 production using Paenibacillus alvei AN 5

Bassam Alkotaini, Nurina Anuar, Abdul Amir H. Kadhum

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Paenibacillus alveiAN5 has been recently isolated from Palm Oil Mill Sludge with the ability to produce a secreted antimicrobial peptide AN5-1. This study indicated the effect of growth factors including incubating temperature, initial pH and different carbon sources on both P. alvei AN5 growth and AN5-1 production. It was noted that, the highest produced amount of AN5-1 were obtained when optimum growth occurred. P.alvei AN5 was grown in Modified Landy Medium at different temperatures; the highest amount produced of AN5-1 was 80 AU/mL at 30°C. In addition, the production was obviously influenced within the pH range of 4-9. At the extreme initial pH values of 4, 5 and 10, the production was depressed. The highest amount produced of AN5-1 (80 AU/mL) was obtained at initial pH of 7. The production of AN5-1 was strongly influenced by the variation of carbon sources, where it was obviously enhanced using glucose; meanwhile, starch and glycerol reduced the production of AN5-1. The minimum glucose concentrations required to achieve the highest amount produced of AN5-1 was 20 g/L. The more P. alvei AN5 grows, the more antimicrobial peptide AN5-1 produces.

Original languageEnglish
Pages (from-to)195-201
Number of pages7
JournalAsian Journal of Microbiology, Biotechnology and Environmental Sciences
Volume15
Issue number1
Publication statusPublished - 2013

Fingerprint

Paenibacillus
peptide
Carbon
Peptides
Temperature
carbon
glucose
temperature
starch
mill
sludge
oil
gastrofenzin
effect
Glucose
Growth
Sewage
Starch
Glycerol

Keywords

  • Antimicrobial peptide AN5-1
  • Glucose
  • Growth factors
  • Paenibacillus alvei AN5

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Environmental Science(all)

Cite this

@article{dfad7fc149ea4335be5da4ca0b88d6ec,
title = "The effects of temperature, pH and carbon sources on antimicrobial peptide AN5-1 production using Paenibacillus alvei AN 5",
abstract = "Paenibacillus alveiAN5 has been recently isolated from Palm Oil Mill Sludge with the ability to produce a secreted antimicrobial peptide AN5-1. This study indicated the effect of growth factors including incubating temperature, initial pH and different carbon sources on both P. alvei AN5 growth and AN5-1 production. It was noted that, the highest produced amount of AN5-1 were obtained when optimum growth occurred. P.alvei AN5 was grown in Modified Landy Medium at different temperatures; the highest amount produced of AN5-1 was 80 AU/mL at 30°C. In addition, the production was obviously influenced within the pH range of 4-9. At the extreme initial pH values of 4, 5 and 10, the production was depressed. The highest amount produced of AN5-1 (80 AU/mL) was obtained at initial pH of 7. The production of AN5-1 was strongly influenced by the variation of carbon sources, where it was obviously enhanced using glucose; meanwhile, starch and glycerol reduced the production of AN5-1. The minimum glucose concentrations required to achieve the highest amount produced of AN5-1 was 20 g/L. The more P. alvei AN5 grows, the more antimicrobial peptide AN5-1 produces.",
keywords = "Antimicrobial peptide AN5-1, Glucose, Growth factors, Paenibacillus alvei AN5",
author = "Bassam Alkotaini and Nurina Anuar and Kadhum, {Abdul Amir H.}",
year = "2013",
language = "English",
volume = "15",
pages = "195--201",
journal = "Asian Journal of Microbiology, Biotechnology and Environmental Sciences",
issn = "0972-3005",
publisher = "Global Publications",
number = "1",

}

TY - JOUR

T1 - The effects of temperature, pH and carbon sources on antimicrobial peptide AN5-1 production using Paenibacillus alvei AN 5

AU - Alkotaini, Bassam

AU - Anuar, Nurina

AU - Kadhum, Abdul Amir H.

PY - 2013

Y1 - 2013

N2 - Paenibacillus alveiAN5 has been recently isolated from Palm Oil Mill Sludge with the ability to produce a secreted antimicrobial peptide AN5-1. This study indicated the effect of growth factors including incubating temperature, initial pH and different carbon sources on both P. alvei AN5 growth and AN5-1 production. It was noted that, the highest produced amount of AN5-1 were obtained when optimum growth occurred. P.alvei AN5 was grown in Modified Landy Medium at different temperatures; the highest amount produced of AN5-1 was 80 AU/mL at 30°C. In addition, the production was obviously influenced within the pH range of 4-9. At the extreme initial pH values of 4, 5 and 10, the production was depressed. The highest amount produced of AN5-1 (80 AU/mL) was obtained at initial pH of 7. The production of AN5-1 was strongly influenced by the variation of carbon sources, where it was obviously enhanced using glucose; meanwhile, starch and glycerol reduced the production of AN5-1. The minimum glucose concentrations required to achieve the highest amount produced of AN5-1 was 20 g/L. The more P. alvei AN5 grows, the more antimicrobial peptide AN5-1 produces.

AB - Paenibacillus alveiAN5 has been recently isolated from Palm Oil Mill Sludge with the ability to produce a secreted antimicrobial peptide AN5-1. This study indicated the effect of growth factors including incubating temperature, initial pH and different carbon sources on both P. alvei AN5 growth and AN5-1 production. It was noted that, the highest produced amount of AN5-1 were obtained when optimum growth occurred. P.alvei AN5 was grown in Modified Landy Medium at different temperatures; the highest amount produced of AN5-1 was 80 AU/mL at 30°C. In addition, the production was obviously influenced within the pH range of 4-9. At the extreme initial pH values of 4, 5 and 10, the production was depressed. The highest amount produced of AN5-1 (80 AU/mL) was obtained at initial pH of 7. The production of AN5-1 was strongly influenced by the variation of carbon sources, where it was obviously enhanced using glucose; meanwhile, starch and glycerol reduced the production of AN5-1. The minimum glucose concentrations required to achieve the highest amount produced of AN5-1 was 20 g/L. The more P. alvei AN5 grows, the more antimicrobial peptide AN5-1 produces.

KW - Antimicrobial peptide AN5-1

KW - Glucose

KW - Growth factors

KW - Paenibacillus alvei AN5

UR - http://www.scopus.com/inward/record.url?scp=84878074686&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878074686&partnerID=8YFLogxK

M3 - Article

VL - 15

SP - 195

EP - 201

JO - Asian Journal of Microbiology, Biotechnology and Environmental Sciences

JF - Asian Journal of Microbiology, Biotechnology and Environmental Sciences

SN - 0972-3005

IS - 1

ER -