The effects of reducing power from metal carbonates on succinic acid production using Actinobacillus succinogenes

Jian Ping Tan, Jamaliah Md Jahim, Ta Yeong Wu, Shuhaida Harun, Tabassum Mumtaz

Research output: Contribution to journalArticle

Abstract

Bio-succinic acid was first commercialized in 2012 and has a potential to hit $ 15 billion market. Higher conversion or yield in this case, allow bio-succinic acid to be more competitive and could potentially take over the market of other intermediate compound. Redox state of the fermentation medium is reported to have an effect on the metabolic flux and in Actinobacillus succinogenes, one of the best succinic acid producers. It has a direct impact on succinate yield. Therefore, carbonates with different reducing power, CaCO3, NaHCO3, MgCO3, ZnCO3 and (NH4)2CO3 were employed to investigate the effect of their reducing power on succinate yield from glucose. Results showed that a more reducing carbonate, CaCO3 promoted the metabolic flux of A. succinogenes to produce higher reductive metabolite (succinate) to regain its intracellular redox balance. Calcium has a reducing power of 2.87 relative to hydrogen. Ammonium carbonate has an oxidizing power of 0.27, or a negative reducing power relative to hydrogen. Therefore, CaCO3 with a stronger reducing power had a higher succinate yield of 0.68 g/g than (NH4)2CO3 with only 0.14 g/g. On the other hand, MgCO3 had the highest final succinate concentration due to the overall higher sugar consumption by A. succinogenes.

Original languageEnglish
Pages (from-to)61-65
Number of pages5
JournalJurnal Teknologi
Volume79
Issue number5-3
DOIs
Publication statusPublished - 2017

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Carbonates
Acids
Metals
Fluxes
Hydrogen
Regain
Metabolites
Sugars
Fermentation
Glucose
Calcium
Oxidation-Reduction

Keywords

  • Bio-succinic acid
  • Fermentation
  • Metabolic fluxes
  • Reducing power
  • Succinate yield

ASJC Scopus subject areas

  • Engineering(all)

Cite this

The effects of reducing power from metal carbonates on succinic acid production using Actinobacillus succinogenes. / Tan, Jian Ping; Md Jahim, Jamaliah; Wu, Ta Yeong; Harun, Shuhaida; Mumtaz, Tabassum.

In: Jurnal Teknologi, Vol. 79, No. 5-3, 2017, p. 61-65.

Research output: Contribution to journalArticle

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