The effects of orchidectomy and supraphysiological testosterone administration on trabecular bone structure and gene expression in rats

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Objective: This study aimed to determine the effects of orchidectomy and supraphysiological testosterone replacement on trabecular structure and gene expression in the bone. Methods: Twenty-four 3-month old male rats were randomized into sham (SH), orchidectomized (ORX) and testosterone-treated (TE) groups. Orchidectomy was performed on the ORX and TE group. Weekly testosterone enanthate intramuscular injection at 7mg/kg body weight was administered to the TE group for 8 weeks while the other groups received peanut oil as vehicle. Blood was collected before and after treatment for serum testosterone analysis. The femora and tibiae were harvested after the treatment period for trabecular structure and gene expression analysis. Results: The trabecular bone volume decreased significantly and the porosity increased significantly in the ORX group compared to the SH group (p<0.05). Testosterone treatment prevented all these changes (p<0.05). The expression of osteogenic genes decreased significantly in the ORX group compared to the SH group (p<0.05). Testosterone treatment decreased the expressions of RANKL and OPG genes significantly (p<0.05). Conclusion: Orchidectomy-induced degeneration in trabecular structure is caused by a decrease in the expressions of osteogenic genes. Supraphysiological testosterone replacement is able to prevent these degenerative changes in the bone despite the modest changes in gene expression.

Original languageEnglish
Pages (from-to)60-66
Number of pages7
JournalAging Male
Volume18
Issue number1
DOIs
Publication statusPublished - 1 Mar 2015

Fingerprint

Orchiectomy
Testosterone
Gene Expression
Bone and Bones
Porosity
Intramuscular Injections
Therapeutics
Tibia
Femur
Cancellous Bone
Body Weight
Serum
Genes

Keywords

  • Bone
  • Male
  • Orchidectomy
  • Osteoporosis
  • Testosterone

ASJC Scopus subject areas

  • Geriatrics and Gerontology

Cite this

@article{549f8c3f428b4effa7d2b74c5a0b4f11,
title = "The effects of orchidectomy and supraphysiological testosterone administration on trabecular bone structure and gene expression in rats",
abstract = "Objective: This study aimed to determine the effects of orchidectomy and supraphysiological testosterone replacement on trabecular structure and gene expression in the bone. Methods: Twenty-four 3-month old male rats were randomized into sham (SH), orchidectomized (ORX) and testosterone-treated (TE) groups. Orchidectomy was performed on the ORX and TE group. Weekly testosterone enanthate intramuscular injection at 7mg/kg body weight was administered to the TE group for 8 weeks while the other groups received peanut oil as vehicle. Blood was collected before and after treatment for serum testosterone analysis. The femora and tibiae were harvested after the treatment period for trabecular structure and gene expression analysis. Results: The trabecular bone volume decreased significantly and the porosity increased significantly in the ORX group compared to the SH group (p<0.05). Testosterone treatment prevented all these changes (p<0.05). The expression of osteogenic genes decreased significantly in the ORX group compared to the SH group (p<0.05). Testosterone treatment decreased the expressions of RANKL and OPG genes significantly (p<0.05). Conclusion: Orchidectomy-induced degeneration in trabecular structure is caused by a decrease in the expressions of osteogenic genes. Supraphysiological testosterone replacement is able to prevent these degenerative changes in the bone despite the modest changes in gene expression.",
keywords = "Bone, Male, Orchidectomy, Osteoporosis, Testosterone",
author = "{Kok Yong}, Chin and Soelaiman, {Ima Nirwana}",
year = "2015",
month = "3",
day = "1",
doi = "10.3109/13685538.2014.954995",
language = "English",
volume = "18",
pages = "60--66",
journal = "Aging Male",
issn = "1368-5538",
publisher = "Informa Healthcare",
number = "1",

}

TY - JOUR

T1 - The effects of orchidectomy and supraphysiological testosterone administration on trabecular bone structure and gene expression in rats

AU - Kok Yong, Chin

AU - Soelaiman, Ima Nirwana

PY - 2015/3/1

Y1 - 2015/3/1

N2 - Objective: This study aimed to determine the effects of orchidectomy and supraphysiological testosterone replacement on trabecular structure and gene expression in the bone. Methods: Twenty-four 3-month old male rats were randomized into sham (SH), orchidectomized (ORX) and testosterone-treated (TE) groups. Orchidectomy was performed on the ORX and TE group. Weekly testosterone enanthate intramuscular injection at 7mg/kg body weight was administered to the TE group for 8 weeks while the other groups received peanut oil as vehicle. Blood was collected before and after treatment for serum testosterone analysis. The femora and tibiae were harvested after the treatment period for trabecular structure and gene expression analysis. Results: The trabecular bone volume decreased significantly and the porosity increased significantly in the ORX group compared to the SH group (p<0.05). Testosterone treatment prevented all these changes (p<0.05). The expression of osteogenic genes decreased significantly in the ORX group compared to the SH group (p<0.05). Testosterone treatment decreased the expressions of RANKL and OPG genes significantly (p<0.05). Conclusion: Orchidectomy-induced degeneration in trabecular structure is caused by a decrease in the expressions of osteogenic genes. Supraphysiological testosterone replacement is able to prevent these degenerative changes in the bone despite the modest changes in gene expression.

AB - Objective: This study aimed to determine the effects of orchidectomy and supraphysiological testosterone replacement on trabecular structure and gene expression in the bone. Methods: Twenty-four 3-month old male rats were randomized into sham (SH), orchidectomized (ORX) and testosterone-treated (TE) groups. Orchidectomy was performed on the ORX and TE group. Weekly testosterone enanthate intramuscular injection at 7mg/kg body weight was administered to the TE group for 8 weeks while the other groups received peanut oil as vehicle. Blood was collected before and after treatment for serum testosterone analysis. The femora and tibiae were harvested after the treatment period for trabecular structure and gene expression analysis. Results: The trabecular bone volume decreased significantly and the porosity increased significantly in the ORX group compared to the SH group (p<0.05). Testosterone treatment prevented all these changes (p<0.05). The expression of osteogenic genes decreased significantly in the ORX group compared to the SH group (p<0.05). Testosterone treatment decreased the expressions of RANKL and OPG genes significantly (p<0.05). Conclusion: Orchidectomy-induced degeneration in trabecular structure is caused by a decrease in the expressions of osteogenic genes. Supraphysiological testosterone replacement is able to prevent these degenerative changes in the bone despite the modest changes in gene expression.

KW - Bone

KW - Male

KW - Orchidectomy

KW - Osteoporosis

KW - Testosterone

UR - http://www.scopus.com/inward/record.url?scp=84924353102&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84924353102&partnerID=8YFLogxK

U2 - 10.3109/13685538.2014.954995

DO - 10.3109/13685538.2014.954995

M3 - Article

C2 - 25166624

AN - SCOPUS:84924353102

VL - 18

SP - 60

EP - 66

JO - Aging Male

JF - Aging Male

SN - 1368-5538

IS - 1

ER -