The effects of magnetic separation on cryopreserved bovine spermatozoa motility, viability and cryo-capacitation status

S. S M Faezah, F. M Y Zuraina, Jaffar Farah Hanan Fathihah, Khairul Osman, N. I. Hilwani, M. I. Iswadi, C. N. Fang, I. Zawawi, O. M. Abas, Siti Fatimah Ibrahim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Summary Cryopreservation is a technique used to preserve cells for long-time storage. It is widely used in agriculture to store male gametes in liquid nitrogen. The aim of this study was to determine the optimum thawing temperature and time for samples subjected to annexin V magnetic-activated cell sorting (AnMACS) as the sperm preparation technique. Pooled semen samples from three ejaculates were divided into two groups. The treatment group was subjected both to AnMACS and to being cryopreserved, whilst the control group was cryopreserved directly without MACS. Post-thaw analysis was carried out for samples thawed at either 20°C for 13 s, 37°C for 30 s, 40°C for 7 s, 60°C for 6 s or 80°C for 5 s. Sperm kinematics, viability and capacitation status were determined for samples subjected to all thawing temperatures described. Results showed that thawing at 37°C for 13 s for MACS-processed samples was a superior option compared with other thawing procedures; there was a significant difference in P < 0.05 values for curvilinear velocity (VCL μm/s) and sperm straightness (STR %) when samples were thawed at 40°C for 7 s, with fewer capacitated spermatozoa (P < 0.05) when samples were thawed at 37°C for 30 s, 40°C for 7 s or 60°C for 6 s. Hence, we can speculate that the use of AnMACS as the sperm preparation technique can somehow enhance sperm cryosurvival rate after cryopreservation, however the fertilization potential of these cells has yet to be determined.

Original languageEnglish
Pages (from-to)378-386
Number of pages9
JournalZygote
Volume22
Issue number3
DOIs
Publication statusPublished - 2014

Fingerprint

Spermatozoa
Annexin A5
Cryopreservation
Temperature
Semen
Agriculture
Biomechanical Phenomena
Fertilization
Germ Cells
Nitrogen
Control Groups
Therapeutics

Keywords

  • Capacitation
  • Magnetic-activated cell sorting system
  • Thawing
  • Viability

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

The effects of magnetic separation on cryopreserved bovine spermatozoa motility, viability and cryo-capacitation status. / Faezah, S. S M; Zuraina, F. M Y; Farah Hanan Fathihah, Jaffar; Osman, Khairul; Hilwani, N. I.; Iswadi, M. I.; Fang, C. N.; Zawawi, I.; Abas, O. M.; Ibrahim, Siti Fatimah.

In: Zygote, Vol. 22, No. 3, 2014, p. 378-386.

Research output: Contribution to journalArticle

Faezah, S. S M ; Zuraina, F. M Y ; Farah Hanan Fathihah, Jaffar ; Osman, Khairul ; Hilwani, N. I. ; Iswadi, M. I. ; Fang, C. N. ; Zawawi, I. ; Abas, O. M. ; Ibrahim, Siti Fatimah. / The effects of magnetic separation on cryopreserved bovine spermatozoa motility, viability and cryo-capacitation status. In: Zygote. 2014 ; Vol. 22, No. 3. pp. 378-386.
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AU - Faezah, S. S M

AU - Zuraina, F. M Y

AU - Farah Hanan Fathihah, Jaffar

AU - Osman, Khairul

AU - Hilwani, N. I.

AU - Iswadi, M. I.

AU - Fang, C. N.

AU - Zawawi, I.

AU - Abas, O. M.

AU - Ibrahim, Siti Fatimah

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N2 - Summary Cryopreservation is a technique used to preserve cells for long-time storage. It is widely used in agriculture to store male gametes in liquid nitrogen. The aim of this study was to determine the optimum thawing temperature and time for samples subjected to annexin V magnetic-activated cell sorting (AnMACS) as the sperm preparation technique. Pooled semen samples from three ejaculates were divided into two groups. The treatment group was subjected both to AnMACS and to being cryopreserved, whilst the control group was cryopreserved directly without MACS. Post-thaw analysis was carried out for samples thawed at either 20°C for 13 s, 37°C for 30 s, 40°C for 7 s, 60°C for 6 s or 80°C for 5 s. Sperm kinematics, viability and capacitation status were determined for samples subjected to all thawing temperatures described. Results showed that thawing at 37°C for 13 s for MACS-processed samples was a superior option compared with other thawing procedures; there was a significant difference in P < 0.05 values for curvilinear velocity (VCL μm/s) and sperm straightness (STR %) when samples were thawed at 40°C for 7 s, with fewer capacitated spermatozoa (P < 0.05) when samples were thawed at 37°C for 30 s, 40°C for 7 s or 60°C for 6 s. Hence, we can speculate that the use of AnMACS as the sperm preparation technique can somehow enhance sperm cryosurvival rate after cryopreservation, however the fertilization potential of these cells has yet to be determined.

AB - Summary Cryopreservation is a technique used to preserve cells for long-time storage. It is widely used in agriculture to store male gametes in liquid nitrogen. The aim of this study was to determine the optimum thawing temperature and time for samples subjected to annexin V magnetic-activated cell sorting (AnMACS) as the sperm preparation technique. Pooled semen samples from three ejaculates were divided into two groups. The treatment group was subjected both to AnMACS and to being cryopreserved, whilst the control group was cryopreserved directly without MACS. Post-thaw analysis was carried out for samples thawed at either 20°C for 13 s, 37°C for 30 s, 40°C for 7 s, 60°C for 6 s or 80°C for 5 s. Sperm kinematics, viability and capacitation status were determined for samples subjected to all thawing temperatures described. Results showed that thawing at 37°C for 13 s for MACS-processed samples was a superior option compared with other thawing procedures; there was a significant difference in P < 0.05 values for curvilinear velocity (VCL μm/s) and sperm straightness (STR %) when samples were thawed at 40°C for 7 s, with fewer capacitated spermatozoa (P < 0.05) when samples were thawed at 37°C for 30 s, 40°C for 7 s or 60°C for 6 s. Hence, we can speculate that the use of AnMACS as the sperm preparation technique can somehow enhance sperm cryosurvival rate after cryopreservation, however the fertilization potential of these cells has yet to be determined.

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