The effects of Labisia pumila extracts on bone microarchitecture of ovariectomized-induced osteoporosis rats: A micro-CT analysis

Nadia Mohd Effendy, Mohd Fadhli Khamis, Ahmad Nazrun Shuid

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

BACKGOUND: Labisia pumila (LP) is a popular herb used by women over the past few decades. This herb has shown potentials as an alternative agent for treatment and prevention of postmenopausal osteoporosis. It was observed in previous studies that supplementation to ovariectomized rats were associated with increased bone antioxidative enzymes and reduced lipid peroxidation activity. It had also improved bone formation markers in ovariectomized rats. This study aimed to evaluate the effects of giving different forms of LP extracts on the trabecular bone microarchitecture of ovariectomised rats. METHODS: Forty-eight female Sprague-Dawley rats were randomly divided into sham-operated (Sham), ovariectomized control (OVX), ovariectomized and given estrogen at 64.5 μg/kg (ERT), ovariectomized and given LP aqueous extract (LPaq), LP methanol extract (LPmet) and LP ethanol extract (LPet) at 100mg/kg, respectively. Treatments were given daily via oral gavages for nine weeks. Following sacrifice, femora were dissected out for bone microarchitectural analysis using an in vitro micro-CT, which provided three dimensional informations on bone microarchitecture. RESULTS: LPaq was the most effective extract found to improve the bone microarchitectural paramaters which comprised ofBone volume fraction (BV/TV), Trabecular separation (Tb.Sp), Trabecular number (Tb.N), Connective density (Conn.dens), Structure model index (SMI) and Degree of anisotropy (DA). CONCLUSION: LPaq was effective in protecting the bone of postmenopausal osteoporosis rat model against microarchitectural deterioration.

Original languageEnglish
Pages (from-to)101-112
Number of pages12
JournalJournal of X-Ray Science and Technology
Volume25
Issue number1
DOIs
Publication statusPublished - 2017

Fingerprint

osteoporosis
bones
Osteoporosis
rats
Rats
Bone
Bone and Bones
Postmenopausal Osteoporosis
osteogenesis
Anisotropy
estrogens
femur
Osteogenesis
Femur
Lipid Peroxidation
Sprague Dawley Rats
Methanol
deterioration
Estrogens
Ethanol

Keywords

  • bone microarchitecture
  • imaging
  • Labisia pumila
  • micro-CT
  • osteoporosis

ASJC Scopus subject areas

  • Radiation
  • Instrumentation
  • Radiology Nuclear Medicine and imaging
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

The effects of Labisia pumila extracts on bone microarchitecture of ovariectomized-induced osteoporosis rats : A micro-CT analysis. / Effendy, Nadia Mohd; Khamis, Mohd Fadhli; Shuid, Ahmad Nazrun.

In: Journal of X-Ray Science and Technology, Vol. 25, No. 1, 2017, p. 101-112.

Research output: Contribution to journalArticle

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