The effects of flavonoids on human phenolsulphotransferases: Potential in drug metabolism and chemoprevention

Ahmad Rohi Ghazali, R. H. Waring

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Flavonoids are frequently found in fruits and vegetables, and are currently under investigation as potential chemopreventive agents. The present study reports the inhibitory effects of six flavonoids, quercetin, genistein, daidzein, equol, (+)-catechin and flavone, on sulphation of p-nitrophenol, a model substrate for the P-form of PST (thermostable, TS) and dopamine, a model substrate for the M-form of PST (thermolabile, TL). Kinetic studies of the PST activity and the inhibitory effects of flavonoids on the P-form of PST activity (using Hanes-Wolf and Dixon plots) show low Km and Ki values. Quercetin was found to be the most potent inhibitor and flavone was the least active inhibitor among the flavonoids. Kinetic analysis showed that the inhibition was non-competitive and Ki values were determined for each flavonoid. These observations suggest the potential for clinically important pharmacological and toxicological interactions by flavonoids.

Original languageEnglish
Pages (from-to)1625-1632
Number of pages8
JournalLife Sciences
Volume65
Issue number16
DOIs
Publication statusPublished - 10 Sep 1999
Externally publishedYes

Fingerprint

Chemoprevention
Flavonoids
Metabolism
flavone
Pharmaceutical Preparations
Quercetin
Equol
Kinetics
Catechin
Genistein
Vegetables
Substrates
Fruits
Toxicology
Fruit
Dopamine
Pharmacology

Keywords

  • (+)-Catechin
  • Diadzein
  • Equol
  • Flavone
  • Flavonoids
  • Genistein
  • Human phenolsulphotransferases
  • Quercetin

ASJC Scopus subject areas

  • Pharmacology

Cite this

The effects of flavonoids on human phenolsulphotransferases : Potential in drug metabolism and chemoprevention. / Ghazali, Ahmad Rohi; Waring, R. H.

In: Life Sciences, Vol. 65, No. 16, 10.09.1999, p. 1625-1632.

Research output: Contribution to journalArticle

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