The effects of Cosmos caudatus (Ulam Raja) on the levels of expression of Nrf2 target genes in mice liver

Azman Abdullah, Tan Chung Phern, Nur Fatihah Hanis Mohamad Rahim, Nur Izzati Zaharuddin, Nurdayana Maulat Salihin, Ahmad Hilmi Yusof, Kamsiah Jaarin, Kamisah Yusof, Qodriyah Mohd Saad, Nur Azlina Mohd Fahami, Harizz Miszard Radman

Research output: Contribution to journalArticle

Abstract

Background: Cosmos caudatus (Ulam Raja) is an appetizer (ulam) eaten with rice in Malaysia. Previous studies showed that Cosmos caudatus possess high antioxidant content. Nrf2 is a transcription factor which regulates the expression of phase II enzymes and antioxidant proteins. The aim of this study is to investigate the effects of Cosmos caudatus aqueous extract (UR) on the expression of Nrf2 target genes in mice liver. Methods: ICR white mice were treated for 21 days with different doses of UR (100, 500, 1000 mg/kg) through oral gavage. Control mice were only given distilled water. After 21 days, the mice were sacrificed and their livers harvested. Total RNA was extracted, reverse transcribed and subjected to qPCR to detect Nrf2 target genes expression. Results: Administration of 100 mg/kg UR significantly increased NQO1 expression in mice liver. Administration of 500 mg/kg UR significantly increased HO-1 liver expression. Administration of 100 and 500 mg/kg UR significantly increased GSTA1 liver expression. Administration of 500 and 1000 mg/kg UR significantly increased GSTM3 liver expression, whereas GSTP and GSTM1 liver expression was significantly decreased at similar doses. Administration of all doses of UR significantly decreased the expression of GSTA3, SOD3 and GCLC in mice liver. Conclusion: UR administration mostly resulted in downregulation of Nrf2 target genes. However, conclusive evidence can only be made through the use of Nrf2 knockout mice or by performing Nrf2 nuclear translocation studies.

Original languageEnglish
Pages (from-to)147-157
Number of pages11
JournalJournal of Pharmacy and Nutrition Sciences
Volume7
Issue number4
DOIs
Publication statusPublished - 1 Jan 2017

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Skates (Fish)
Liver
Genes
Antioxidants
Inbred ICR Mouse
Malaysia
Knockout Mice
water
Transcription Factors
Down-Regulation
RNA
Gene Expression
evidence

Keywords

  • Cosmos caudatus
  • Gene expression
  • Liver
  • Mice
  • Nrf2

ASJC Scopus subject areas

  • Health(social science)
  • Medicine (miscellaneous)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Nutrition and Dietetics

Cite this

The effects of Cosmos caudatus (Ulam Raja) on the levels of expression of Nrf2 target genes in mice liver. / Abdullah, Azman; Phern, Tan Chung; Rahim, Nur Fatihah Hanis Mohamad; Zaharuddin, Nur Izzati; Salihin, Nurdayana Maulat; Yusof, Ahmad Hilmi; Jaarin, Kamsiah; Yusof, Kamisah; Mohd Saad, Qodriyah; Mohd Fahami, Nur Azlina; Radman, Harizz Miszard.

In: Journal of Pharmacy and Nutrition Sciences, Vol. 7, No. 4, 01.01.2017, p. 147-157.

Research output: Contribution to journalArticle

Abdullah, Azman ; Phern, Tan Chung ; Rahim, Nur Fatihah Hanis Mohamad ; Zaharuddin, Nur Izzati ; Salihin, Nurdayana Maulat ; Yusof, Ahmad Hilmi ; Jaarin, Kamsiah ; Yusof, Kamisah ; Mohd Saad, Qodriyah ; Mohd Fahami, Nur Azlina ; Radman, Harizz Miszard. / The effects of Cosmos caudatus (Ulam Raja) on the levels of expression of Nrf2 target genes in mice liver. In: Journal of Pharmacy and Nutrition Sciences. 2017 ; Vol. 7, No. 4. pp. 147-157.
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abstract = "Background: Cosmos caudatus (Ulam Raja) is an appetizer (ulam) eaten with rice in Malaysia. Previous studies showed that Cosmos caudatus possess high antioxidant content. Nrf2 is a transcription factor which regulates the expression of phase II enzymes and antioxidant proteins. The aim of this study is to investigate the effects of Cosmos caudatus aqueous extract (UR) on the expression of Nrf2 target genes in mice liver. Methods: ICR white mice were treated for 21 days with different doses of UR (100, 500, 1000 mg/kg) through oral gavage. Control mice were only given distilled water. After 21 days, the mice were sacrificed and their livers harvested. Total RNA was extracted, reverse transcribed and subjected to qPCR to detect Nrf2 target genes expression. Results: Administration of 100 mg/kg UR significantly increased NQO1 expression in mice liver. Administration of 500 mg/kg UR significantly increased HO-1 liver expression. Administration of 100 and 500 mg/kg UR significantly increased GSTA1 liver expression. Administration of 500 and 1000 mg/kg UR significantly increased GSTM3 liver expression, whereas GSTP and GSTM1 liver expression was significantly decreased at similar doses. Administration of all doses of UR significantly decreased the expression of GSTA3, SOD3 and GCLC in mice liver. Conclusion: UR administration mostly resulted in downregulation of Nrf2 target genes. However, conclusive evidence can only be made through the use of Nrf2 knockout mice or by performing Nrf2 nuclear translocation studies.",
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AU - Rahim, Nur Fatihah Hanis Mohamad

AU - Zaharuddin, Nur Izzati

AU - Salihin, Nurdayana Maulat

AU - Yusof, Ahmad Hilmi

AU - Jaarin, Kamsiah

AU - Yusof, Kamisah

AU - Mohd Saad, Qodriyah

AU - Mohd Fahami, Nur Azlina

AU - Radman, Harizz Miszard

PY - 2017/1/1

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N2 - Background: Cosmos caudatus (Ulam Raja) is an appetizer (ulam) eaten with rice in Malaysia. Previous studies showed that Cosmos caudatus possess high antioxidant content. Nrf2 is a transcription factor which regulates the expression of phase II enzymes and antioxidant proteins. The aim of this study is to investigate the effects of Cosmos caudatus aqueous extract (UR) on the expression of Nrf2 target genes in mice liver. Methods: ICR white mice were treated for 21 days with different doses of UR (100, 500, 1000 mg/kg) through oral gavage. Control mice were only given distilled water. After 21 days, the mice were sacrificed and their livers harvested. Total RNA was extracted, reverse transcribed and subjected to qPCR to detect Nrf2 target genes expression. Results: Administration of 100 mg/kg UR significantly increased NQO1 expression in mice liver. Administration of 500 mg/kg UR significantly increased HO-1 liver expression. Administration of 100 and 500 mg/kg UR significantly increased GSTA1 liver expression. Administration of 500 and 1000 mg/kg UR significantly increased GSTM3 liver expression, whereas GSTP and GSTM1 liver expression was significantly decreased at similar doses. Administration of all doses of UR significantly decreased the expression of GSTA3, SOD3 and GCLC in mice liver. Conclusion: UR administration mostly resulted in downregulation of Nrf2 target genes. However, conclusive evidence can only be made through the use of Nrf2 knockout mice or by performing Nrf2 nuclear translocation studies.

AB - Background: Cosmos caudatus (Ulam Raja) is an appetizer (ulam) eaten with rice in Malaysia. Previous studies showed that Cosmos caudatus possess high antioxidant content. Nrf2 is a transcription factor which regulates the expression of phase II enzymes and antioxidant proteins. The aim of this study is to investigate the effects of Cosmos caudatus aqueous extract (UR) on the expression of Nrf2 target genes in mice liver. Methods: ICR white mice were treated for 21 days with different doses of UR (100, 500, 1000 mg/kg) through oral gavage. Control mice were only given distilled water. After 21 days, the mice were sacrificed and their livers harvested. Total RNA was extracted, reverse transcribed and subjected to qPCR to detect Nrf2 target genes expression. Results: Administration of 100 mg/kg UR significantly increased NQO1 expression in mice liver. Administration of 500 mg/kg UR significantly increased HO-1 liver expression. Administration of 100 and 500 mg/kg UR significantly increased GSTA1 liver expression. Administration of 500 and 1000 mg/kg UR significantly increased GSTM3 liver expression, whereas GSTP and GSTM1 liver expression was significantly decreased at similar doses. Administration of all doses of UR significantly decreased the expression of GSTA3, SOD3 and GCLC in mice liver. Conclusion: UR administration mostly resulted in downregulation of Nrf2 target genes. However, conclusive evidence can only be made through the use of Nrf2 knockout mice or by performing Nrf2 nuclear translocation studies.

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KW - Gene expression

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KW - Nrf2

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