The effects of calcium on the swelling pressure (Ps) vs hydration relationship in bovine corneal stroma

Bariah Mohd. Ali, Noriko Fushimi, Joseph W. Huff

Research output: Contribution to journalArticle

Abstract

Purpose: Stromal anionic groups are largely responsible for stromal hydration and bind Ca++. Ca++ binding to these groups might therefore inhibit fluid imbibition. We compared the effects of the chloride salts of Ca++ and other cations on the Ps vs hydration relationship. Methods: Bovine stromas were pre-equilibrated 30 min with 0.154 M CaCl2, NaCl, KCl, or NH4Cl buffered with 10 mM MOPS and pressed in the same solutions between glass filter disks at Ps values of 33-150 mm Hg. At equilibrium (3 hr), hydration was measured gravimetrically. Regression analysis of log(Ps) vs hydration was calculated and slopes and Y intercepts of regressions were compared using ANOVA and the Student Neuman Keuls test. Slopes reflect log(Ps)/Hydration, and Y intercepts indicate (extrapolated) log(swelling pressure) at zero hydration. Results: Slopes were identical for NaCl, KCl and NH4Cl, but were significantly reduced (p < 0.05) for CaCl2. A similar finding was seen with Y intercepts. Calcium consistently reduces stromal hydration at these swelling pressures. Slopes and Elevations of log(Swelling Pressure) vs Hydration NaCl KCl NH4Cl CaCl2 Slope ± SD -2.72 ± 0.22 -2.65 ± 0.27 -2.74 ± 0.35 -1.70 ± 0.28* Y intercept ± SD 9.06 ± 0.18 8.96 ± 0.22 9.48 ± 0.29 6.45 ± 0.24* † Mean and SD for 15 corneas/group * p<0.05 vs all other salts Conclusions: CaCl2 inhibits stromal fluid imbibition, likely due to shielding or bonding to anionic groups. Understanding the mechanism of this effect and its structure-activity-relationships may lead to the development of improved corneal irrigation solutions, eye bank storage media and therapies for corneal edema.

Original languageEnglish
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
Publication statusPublished - 15 Feb 1996
Externally publishedYes

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Corneal Stroma
Calcium
Pressure
Salts
Eye Banks
Corneal Edema
Structure-Activity Relationship
Cornea
Glass
Cations
Chlorides
Analysis of Variance
Regression Analysis
Students

ASJC Scopus subject areas

  • Ophthalmology

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The effects of calcium on the swelling pressure (Ps) vs hydration relationship in bovine corneal stroma. / Mohd. Ali, Bariah; Fushimi, Noriko; Huff, Joseph W.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.

Research output: Contribution to journalArticle

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abstract = "Purpose: Stromal anionic groups are largely responsible for stromal hydration and bind Ca++. Ca++ binding to these groups might therefore inhibit fluid imbibition. We compared the effects of the chloride salts of Ca++ and other cations on the Ps vs hydration relationship. Methods: Bovine stromas were pre-equilibrated 30 min with 0.154 M CaCl2, NaCl, KCl, or NH4Cl buffered with 10 mM MOPS and pressed in the same solutions between glass filter disks at Ps values of 33-150 mm Hg. At equilibrium (3 hr), hydration was measured gravimetrically. Regression analysis of log(Ps) vs hydration was calculated and slopes and Y intercepts of regressions were compared using ANOVA and the Student Neuman Keuls test. Slopes reflect log(Ps)/Hydration, and Y intercepts indicate (extrapolated) log(swelling pressure) at zero hydration. Results: Slopes were identical for NaCl, KCl and NH4Cl, but were significantly reduced (p < 0.05) for CaCl2. A similar finding was seen with Y intercepts. Calcium consistently reduces stromal hydration at these swelling pressures. Slopes and Elevations of log(Swelling Pressure) vs Hydration† NaCl KCl NH4Cl CaCl2 Slope ± SD -2.72 ± 0.22 -2.65 ± 0.27 -2.74 ± 0.35 -1.70 ± 0.28* Y intercept ± SD 9.06 ± 0.18 8.96 ± 0.22 9.48 ± 0.29 6.45 ± 0.24* † Mean and SD for 15 corneas/group * p<0.05 vs all other salts Conclusions: CaCl2 inhibits stromal fluid imbibition, likely due to shielding or bonding to anionic groups. Understanding the mechanism of this effect and its structure-activity-relationships may lead to the development of improved corneal irrigation solutions, eye bank storage media and therapies for corneal edema.",
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