The effects of age on monolayer culture of human keratinocytes for future use in skin engineering.

B. H. Muhd Fakhruddin, B. S. Aminuddin, Mazlyzam Abdul Latif, Ruszymah Idrus

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Skin is the largest organ in human system and plays a vital role as a barrier against environment and pathogens. Skin regeneration is important in tissue engineering especially in cases of chronic wounds. With the tissue engineering technology, these skins equivalent have been use clinically to repair burns and wounds. Consented redundant skin samples were obtained from patients aged 9 to 65 years old. Skin samples were digested with dispase, thus separating the epidermis and the dermis layer. The epidermis layer was trypsinized and cultured in DKSFM in 6-well plate at 37 degrees C and 5% CO2. Once confluent, the culture were trypsinized and the cells were pooled. Cells were counted using haemacytometer. Doubling time and viability were calculated and analysed. From the result, we conclude that doubling time and viability of in vitro keratinocytes cultured in DKSFM media is not age dependant.

Original languageEnglish
Pages (from-to)182-183
Number of pages2
JournalThe Medical journal of Malaysia
Volume59 Suppl B
Publication statusPublished - May 2004

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Keratinocytes
Skin
Tissue Engineering
Epidermis
Wounds and Injuries
Dermis
Burns
Regeneration
Technology

ASJC Scopus subject areas

  • Medicine(all)

Cite this

The effects of age on monolayer culture of human keratinocytes for future use in skin engineering. / Muhd Fakhruddin, B. H.; Aminuddin, B. S.; Abdul Latif, Mazlyzam; Idrus, Ruszymah.

In: The Medical journal of Malaysia, Vol. 59 Suppl B, 05.2004, p. 182-183.

Research output: Contribution to journalArticle

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