The effectiveness of gentamicin against acanthamoeba cysts in vitro

S. A. Noradilah, Mohamed Kamel Abdul Ghani, Anisah Nordin, A. R. Noraina, S. Yusof

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Acanthamoeba is a free-living protozoa which causes serious ocular problem. Acanthamoeba keratitis is becoming more prevalent amongst contact lens wearers and it can cause loss of vision and blindness if not treated properly. The objective of this research is to determine the effectiveness of gentamicin against six Acanthamoeba spp. isolates, of which three were clinical isolates (HS 6, HKL 95, HTH 73) and three environmental isolates (SMAL 7, SMAL 8, TTT 9). Cyst suspension from the chosen isolates were exposed to gentamicin. After 48 hours of incubation at temperature of 30°C and 37°C, each mixture was filtered and filtration membrane was put onto non-nutrient agar laid with Escherichia coli. The agar plates were incubated for three days at 30oC and 37oC and the plates were examined daily until day 14 to look for the presence of Acanthamoeba trophozoites under inverted microscope. The presence of trophozoites indicated the ineffectiveness of gentamicin. Gentamicin was found to be effective against Acanthamoeba cysts from all the test strains at both incubation temperatures. The minimum cysticidal concentration (MCC) mean value of gentamicin was 0.193 mg/mL at 30oC and 0.229 mg/mL at 37oC. So, we concluded that gentamicin has cysticidal potential towards Acanthamoeba.

Original languageEnglish
Pages (from-to)51-54
Number of pages4
JournalMalaysian Journal of Medicine and Health Sciences
Volume8
Issue number2
Publication statusPublished - Jun 2012

Fingerprint

Acanthamoeba
Gentamicins
Cysts
Trophozoites
HS 6
Agar
Acanthamoeba Keratitis
Temperature
Contact Lenses
Blindness
In Vitro Techniques
Suspensions
Escherichia coli
Membranes
Research

Keywords

  • Acanthamoeba
  • Gentamicin
  • Sensitivity

ASJC Scopus subject areas

  • Medicine(all)

Cite this

The effectiveness of gentamicin against acanthamoeba cysts in vitro. / Noradilah, S. A.; Abdul Ghani, Mohamed Kamel; Nordin, Anisah; Noraina, A. R.; Yusof, S.

In: Malaysian Journal of Medicine and Health Sciences, Vol. 8, No. 2, 06.2012, p. 51-54.

Research output: Contribution to journalArticle

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