The effectiveness of contact lens disinfecting solutions against Acanthamoeba Cysts

Mohamed Kamel Abdul Ghani, Kirat Kaur Santokh Singh, Anisah Nordin, Yusof Suboh, Noraina Ab Rahim, Norazah Ahmad

Research output: Contribution to journalArticle

Abstract

Introduction: The association between Acanthamoeba keratitis and contact lens wear is now firmly established. Thus, the use of contact lens disinfecting solutions effective at killing Acanthamoeba organisms is important in preventing corneal infections. However the effectiveness of commercially available contact lens disinfecting solutions as anti-Acanthamoeba is doubtful. Objective: This study investigates the effectiveness of Meni Care Plus and Opti-Free® Express® against 4 clinical isolates of Acanthamoeba sp., to determine their efficacy based on the soaking times recommended by the manufacturers and soaking times of 4 hours, 6 hours and 8 hours. Materials and methods: Cyst suspensions were prepared using Acanthamoeba cysts grown on Non-Nutrient Agar (NNA) for 11 days at 30°C (± 2°C). Meni Care Plus and Opti-Free® Express® were tested to determine their effectiveness as anti-Acanthamoeba agents. Cyst suspensions of the isolates were exposed to each contact lens disinfecting solution based on the manufacturers' recommended soaking time, 4 hours, 6 hours and 8 hours respectively. After the soaking time, 100 μl cyst suspension of each isolate was cultured onto NNA plates seeded with E. coli at 30°C (± 2°C) and observed daily for 14 days under an inverted microscope to detect the presence of trophozoites. Results: Meni Care Plus was successful at inactivating cysts of two clinical isolates after soaking for 6 hours and 8 hours. Opti-Free® Express® was ineffective at inactivating the Acanthamoeba cysts within the testing times. Conclusion: Meni Care Plus displayed partial effectiveness as anti-Acanthamoeba. Most commercially available contact lens disinfecting solutions are ineffective as anti-Acanthamoeba agents.

Original languageEnglish
Pages (from-to)108-111
Number of pages4
JournalInternational Medical Journal
Volume25
Issue number2
Publication statusPublished - 1 Apr 2018

Fingerprint

Contact Lens Solutions
Acanthamoeba
Cysts
Suspensions
Agar
Acanthamoeba Keratitis
Trophozoites
Contact Lenses
Escherichia coli

Keywords

  • Acanthamoeba
  • Contact lens disinfecting solutions
  • Malaysia

ASJC Scopus subject areas

  • Medicine(all)

Cite this

The effectiveness of contact lens disinfecting solutions against Acanthamoeba Cysts. / Abdul Ghani, Mohamed Kamel; Singh, Kirat Kaur Santokh; Nordin, Anisah; Suboh, Yusof; Rahim, Noraina Ab; Ahmad, Norazah.

In: International Medical Journal, Vol. 25, No. 2, 01.04.2018, p. 108-111.

Research output: Contribution to journalArticle

Abdul Ghani, Mohamed Kamel ; Singh, Kirat Kaur Santokh ; Nordin, Anisah ; Suboh, Yusof ; Rahim, Noraina Ab ; Ahmad, Norazah. / The effectiveness of contact lens disinfecting solutions against Acanthamoeba Cysts. In: International Medical Journal. 2018 ; Vol. 25, No. 2. pp. 108-111.
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