The effect of Ulam raja (Cosmos Caudatus) on Drugmetabolizing Enzymes, Lipid Peroxidation and Antioxidant status in mice liver

Harizz Miszard Radman, Kamisah Yusof, Qodriyah Mohd Saad, Wan Zurinah Wan Ngah, Azman Abdullah

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Recent studies indicated that C. caudatus, a herbal plant, possesses strong antioxidant and free radical scavenging activities. It might also have potential tumour-inhibitory effect. This study was conducted to investigate the effects of C. caudatus on drug-metabolizing enzymes (DME), antioxidant status and lipid peroxidation in mice liver. The extent of liver injury was assessed by measuring lactate dehydrogenase (LDH) activity. This study involved 30 adult ICR male mice (25-35 grams) that were divided into 5 groups. Aqueous extract of C. caudatus (CC) were administered orally to mice for 21 days in three different doses [100, 500 and 1000 mg/kg body weight respectively]. Positive control mice were given diet containing 0.5% butylated hydroxyanisole (BHA). After 21 days, the mice were sacrificed and their livers harvested. The results showed that NADPH-cytochrome P450 reductase activity was significantly increased only in mice treated with 500 mg/kg CC compared to the control group. DT-diaphorase (DTD) activity was significantly increased only in mice treated with 1000 mg/kg CC. Superoxide dismutase (SOD) activity was significantly increased only in mice treated with 500 mg/kg CC. Catalase (CAT) activity was significantly increased in mice treated with 1000 mg/kg CC. MDA level (which indicated the extent of lipid peroxidation) was significantly reduced in all CCtreated groups. Lactate dehydrogenase (LDH) level (which indicated the extent of liver injury) was significantly reduced in all CC-treated groups. In conclusion, Cosmos caudatus supplementation might be able to protect mice livers against damage caused by oxidative stress mainly through inhibition of the lipid peroxidation process and LDH activity, as well as through some modulation of the activities of certain antioxidant enzymes.

Original languageEnglish
Pages (from-to)1213-1225
Number of pages13
JournalInternational Journal of PharmTech Research
Volume6
Issue number4
Publication statusPublished - 2014

Fingerprint

Skates (Fish)
Lipid Peroxidation
Antioxidants
Liver
Enzymes
L-Lactate Dehydrogenase
NAD(P)H Dehydrogenase (Quinone)
Butylated Hydroxyanisole
NADPH-Ferrihemoprotein Reductase
Inbred ICR Mouse
Wounds and Injuries
Catalase
Superoxide Dismutase
Free Radicals
Oxidative Stress
Body Weight
Diet

Keywords

  • Antioxidant status
  • Cosmos caudatus
  • Drug-metabolizing enzymes
  • Lipid peroxidation
  • Liver

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology

Cite this

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title = "The effect of Ulam raja (Cosmos Caudatus) on Drugmetabolizing Enzymes, Lipid Peroxidation and Antioxidant status in mice liver",
abstract = "Recent studies indicated that C. caudatus, a herbal plant, possesses strong antioxidant and free radical scavenging activities. It might also have potential tumour-inhibitory effect. This study was conducted to investigate the effects of C. caudatus on drug-metabolizing enzymes (DME), antioxidant status and lipid peroxidation in mice liver. The extent of liver injury was assessed by measuring lactate dehydrogenase (LDH) activity. This study involved 30 adult ICR male mice (25-35 grams) that were divided into 5 groups. Aqueous extract of C. caudatus (CC) were administered orally to mice for 21 days in three different doses [100, 500 and 1000 mg/kg body weight respectively]. Positive control mice were given diet containing 0.5{\%} butylated hydroxyanisole (BHA). After 21 days, the mice were sacrificed and their livers harvested. The results showed that NADPH-cytochrome P450 reductase activity was significantly increased only in mice treated with 500 mg/kg CC compared to the control group. DT-diaphorase (DTD) activity was significantly increased only in mice treated with 1000 mg/kg CC. Superoxide dismutase (SOD) activity was significantly increased only in mice treated with 500 mg/kg CC. Catalase (CAT) activity was significantly increased in mice treated with 1000 mg/kg CC. MDA level (which indicated the extent of lipid peroxidation) was significantly reduced in all CCtreated groups. Lactate dehydrogenase (LDH) level (which indicated the extent of liver injury) was significantly reduced in all CC-treated groups. In conclusion, Cosmos caudatus supplementation might be able to protect mice livers against damage caused by oxidative stress mainly through inhibition of the lipid peroxidation process and LDH activity, as well as through some modulation of the activities of certain antioxidant enzymes.",
keywords = "Antioxidant status, Cosmos caudatus, Drug-metabolizing enzymes, Lipid peroxidation, Liver",
author = "Radman, {Harizz Miszard} and Kamisah Yusof and {Mohd Saad}, Qodriyah and Ngah, {Wan Zurinah Wan} and Azman Abdullah",
year = "2014",
language = "English",
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T1 - The effect of Ulam raja (Cosmos Caudatus) on Drugmetabolizing Enzymes, Lipid Peroxidation and Antioxidant status in mice liver

AU - Radman, Harizz Miszard

AU - Yusof, Kamisah

AU - Mohd Saad, Qodriyah

AU - Ngah, Wan Zurinah Wan

AU - Abdullah, Azman

PY - 2014

Y1 - 2014

N2 - Recent studies indicated that C. caudatus, a herbal plant, possesses strong antioxidant and free radical scavenging activities. It might also have potential tumour-inhibitory effect. This study was conducted to investigate the effects of C. caudatus on drug-metabolizing enzymes (DME), antioxidant status and lipid peroxidation in mice liver. The extent of liver injury was assessed by measuring lactate dehydrogenase (LDH) activity. This study involved 30 adult ICR male mice (25-35 grams) that were divided into 5 groups. Aqueous extract of C. caudatus (CC) were administered orally to mice for 21 days in three different doses [100, 500 and 1000 mg/kg body weight respectively]. Positive control mice were given diet containing 0.5% butylated hydroxyanisole (BHA). After 21 days, the mice were sacrificed and their livers harvested. The results showed that NADPH-cytochrome P450 reductase activity was significantly increased only in mice treated with 500 mg/kg CC compared to the control group. DT-diaphorase (DTD) activity was significantly increased only in mice treated with 1000 mg/kg CC. Superoxide dismutase (SOD) activity was significantly increased only in mice treated with 500 mg/kg CC. Catalase (CAT) activity was significantly increased in mice treated with 1000 mg/kg CC. MDA level (which indicated the extent of lipid peroxidation) was significantly reduced in all CCtreated groups. Lactate dehydrogenase (LDH) level (which indicated the extent of liver injury) was significantly reduced in all CC-treated groups. In conclusion, Cosmos caudatus supplementation might be able to protect mice livers against damage caused by oxidative stress mainly through inhibition of the lipid peroxidation process and LDH activity, as well as through some modulation of the activities of certain antioxidant enzymes.

AB - Recent studies indicated that C. caudatus, a herbal plant, possesses strong antioxidant and free radical scavenging activities. It might also have potential tumour-inhibitory effect. This study was conducted to investigate the effects of C. caudatus on drug-metabolizing enzymes (DME), antioxidant status and lipid peroxidation in mice liver. The extent of liver injury was assessed by measuring lactate dehydrogenase (LDH) activity. This study involved 30 adult ICR male mice (25-35 grams) that were divided into 5 groups. Aqueous extract of C. caudatus (CC) were administered orally to mice for 21 days in three different doses [100, 500 and 1000 mg/kg body weight respectively]. Positive control mice were given diet containing 0.5% butylated hydroxyanisole (BHA). After 21 days, the mice were sacrificed and their livers harvested. The results showed that NADPH-cytochrome P450 reductase activity was significantly increased only in mice treated with 500 mg/kg CC compared to the control group. DT-diaphorase (DTD) activity was significantly increased only in mice treated with 1000 mg/kg CC. Superoxide dismutase (SOD) activity was significantly increased only in mice treated with 500 mg/kg CC. Catalase (CAT) activity was significantly increased in mice treated with 1000 mg/kg CC. MDA level (which indicated the extent of lipid peroxidation) was significantly reduced in all CCtreated groups. Lactate dehydrogenase (LDH) level (which indicated the extent of liver injury) was significantly reduced in all CC-treated groups. In conclusion, Cosmos caudatus supplementation might be able to protect mice livers against damage caused by oxidative stress mainly through inhibition of the lipid peroxidation process and LDH activity, as well as through some modulation of the activities of certain antioxidant enzymes.

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