Abstract
Recent studies indicated that C. caudatus, a herbal plant, possesses strong antioxidant and free radical scavenging activities. It might also have potential tumour-inhibitory effect. This study was conducted to investigate the effects of C. caudatus on drug-metabolizing enzymes (DME), antioxidant status and lipid peroxidation in mice liver. The extent of liver injury was assessed by measuring lactate dehydrogenase (LDH) activity. This study involved 30 adult ICR male mice (25-35 grams) that were divided into 5 groups. Aqueous extract of C. caudatus (CC) were administered orally to mice for 21 days in three different doses [100, 500 and 1000 mg/kg body weight respectively]. Positive control mice were given diet containing 0.5% butylated hydroxyanisole (BHA). After 21 days, the mice were sacrificed and their livers harvested. The results showed that NADPH-cytochrome P450 reductase activity was significantly increased only in mice treated with 500 mg/kg CC compared to the control group. DT-diaphorase (DTD) activity was significantly increased only in mice treated with 1000 mg/kg CC. Superoxide dismutase (SOD) activity was significantly increased only in mice treated with 500 mg/kg CC. Catalase (CAT) activity was significantly increased in mice treated with 1000 mg/kg CC. MDA level (which indicated the extent of lipid peroxidation) was significantly reduced in all CCtreated groups. Lactate dehydrogenase (LDH) level (which indicated the extent of liver injury) was significantly reduced in all CC-treated groups. In conclusion, Cosmos caudatus supplementation might be able to protect mice livers against damage caused by oxidative stress mainly through inhibition of the lipid peroxidation process and LDH activity, as well as through some modulation of the activities of certain antioxidant enzymes.
| Original language | English |
|---|---|
| Pages (from-to) | 1213-1225 |
| Number of pages | 13 |
| Journal | International Journal of PharmTech Research |
| Volume | 6 |
| Issue number | 4 |
| Publication status | Published - 2014 |
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Keywords
- Antioxidant status
- Cosmos caudatus
- Drug-metabolizing enzymes
- Lipid peroxidation
- Liver
ASJC Scopus subject areas
- Pharmaceutical Science
- Pharmacology
Cite this
The effect of Ulam raja (Cosmos Caudatus) on Drugmetabolizing Enzymes, Lipid Peroxidation and Antioxidant status in mice liver. / Radman, Harizz Miszard; Yusof, Kamisah; Mohd Saad, Qodriyah; Ngah, Wan Zurinah Wan; Abdullah, Azman.
In: International Journal of PharmTech Research, Vol. 6, No. 4, 2014, p. 1213-1225.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The effect of Ulam raja (Cosmos Caudatus) on Drugmetabolizing Enzymes, Lipid Peroxidation and Antioxidant status in mice liver
AU - Radman, Harizz Miszard
AU - Yusof, Kamisah
AU - Mohd Saad, Qodriyah
AU - Ngah, Wan Zurinah Wan
AU - Abdullah, Azman
PY - 2014
Y1 - 2014
N2 - Recent studies indicated that C. caudatus, a herbal plant, possesses strong antioxidant and free radical scavenging activities. It might also have potential tumour-inhibitory effect. This study was conducted to investigate the effects of C. caudatus on drug-metabolizing enzymes (DME), antioxidant status and lipid peroxidation in mice liver. The extent of liver injury was assessed by measuring lactate dehydrogenase (LDH) activity. This study involved 30 adult ICR male mice (25-35 grams) that were divided into 5 groups. Aqueous extract of C. caudatus (CC) were administered orally to mice for 21 days in three different doses [100, 500 and 1000 mg/kg body weight respectively]. Positive control mice were given diet containing 0.5% butylated hydroxyanisole (BHA). After 21 days, the mice were sacrificed and their livers harvested. The results showed that NADPH-cytochrome P450 reductase activity was significantly increased only in mice treated with 500 mg/kg CC compared to the control group. DT-diaphorase (DTD) activity was significantly increased only in mice treated with 1000 mg/kg CC. Superoxide dismutase (SOD) activity was significantly increased only in mice treated with 500 mg/kg CC. Catalase (CAT) activity was significantly increased in mice treated with 1000 mg/kg CC. MDA level (which indicated the extent of lipid peroxidation) was significantly reduced in all CCtreated groups. Lactate dehydrogenase (LDH) level (which indicated the extent of liver injury) was significantly reduced in all CC-treated groups. In conclusion, Cosmos caudatus supplementation might be able to protect mice livers against damage caused by oxidative stress mainly through inhibition of the lipid peroxidation process and LDH activity, as well as through some modulation of the activities of certain antioxidant enzymes.
AB - Recent studies indicated that C. caudatus, a herbal plant, possesses strong antioxidant and free radical scavenging activities. It might also have potential tumour-inhibitory effect. This study was conducted to investigate the effects of C. caudatus on drug-metabolizing enzymes (DME), antioxidant status and lipid peroxidation in mice liver. The extent of liver injury was assessed by measuring lactate dehydrogenase (LDH) activity. This study involved 30 adult ICR male mice (25-35 grams) that were divided into 5 groups. Aqueous extract of C. caudatus (CC) were administered orally to mice for 21 days in three different doses [100, 500 and 1000 mg/kg body weight respectively]. Positive control mice were given diet containing 0.5% butylated hydroxyanisole (BHA). After 21 days, the mice were sacrificed and their livers harvested. The results showed that NADPH-cytochrome P450 reductase activity was significantly increased only in mice treated with 500 mg/kg CC compared to the control group. DT-diaphorase (DTD) activity was significantly increased only in mice treated with 1000 mg/kg CC. Superoxide dismutase (SOD) activity was significantly increased only in mice treated with 500 mg/kg CC. Catalase (CAT) activity was significantly increased in mice treated with 1000 mg/kg CC. MDA level (which indicated the extent of lipid peroxidation) was significantly reduced in all CCtreated groups. Lactate dehydrogenase (LDH) level (which indicated the extent of liver injury) was significantly reduced in all CC-treated groups. In conclusion, Cosmos caudatus supplementation might be able to protect mice livers against damage caused by oxidative stress mainly through inhibition of the lipid peroxidation process and LDH activity, as well as through some modulation of the activities of certain antioxidant enzymes.
KW - Antioxidant status
KW - Cosmos caudatus
KW - Drug-metabolizing enzymes
KW - Lipid peroxidation
KW - Liver
UR - http://www.scopus.com/inward/record.url?scp=84905018125&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84905018125&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:84905018125
VL - 6
SP - 1213
EP - 1225
JO - International Journal of PharmTech Research
JF - International Journal of PharmTech Research
SN - 0974-4304
IS - 4
ER -