The effect of Piper sarmentosum on the epicardial fat of the dexamethasone treated Sprague-Dawley rats

Tioh Beng Siong, Muhammad Aizuddin Bin Ahmad, Siti Nor Badriah Binti Isa, Drend Anak Jores, Hasni Syamila Binti Abdul Kadir, Phoong Yee Pin, Faizah Othman, Farihah Suhaimi

Research output: Contribution to journalArticle

Abstract

Objective: Medical treatments using dexamethasone exposes the body to a higher level of glucocorticoid, which then causes increased body adiposity, hyperinsulinemia and insulin resistance. It leads to obesity and further increases the epicardial fat thickness. This "ectopic fat storage syndrome" in the heart will subsequently contribute to the development of cardiovascular diseases. Piper sarmentosum, locally known as Kaduk is a traditional herb rich in natural antioxidants but its effectiveness of inhibiting the "ectopic fat storage syndrome" has not been studied. Therefore, it is our interest to determine the effect of Piper sarmentosum on the histological changes and histomorphometry in the architecture of the epicardial fat of the dexamethasone treated male adrenalectomized rats. Methods: Twenty-four male Sprague-Dawley rats, (2 months old) were divided into Baseline (B), Sham-operated (S), Adrenalectomized Control (C) and Adrenalectomized Piper sarmentosum (K) groups. Only the C and K groups received daily treatment of intramuscular dexamethasone (120 μg/kg/day) for a period of two months. Group K also received Piper sarmentosum extract (0.125 g/kg/day) during the same period. The animals were sacrificed and the heart tissues were taken and fixed in 10% formalin for light microscopy. Results: Results demonstrated that the epicardial fat thickness (p < 0.05) and adipocytes number (p < 0.05) in K were significantly reduced when compared with C. Conclusion: Thus, these results imply that there is a potential role for Piper sarmentosum in inhibiting or delaying the "ectopic fat storage syndrome" in dexamethasone treatment.

Original languageEnglish
Pages (from-to)223-227
Number of pages5
JournalInternational Medical Journal
Volume17
Issue number3
Publication statusPublished - Sep 2010

Fingerprint

Piper
Dexamethasone
Sprague Dawley Rats
Fats
Adiposity
Hyperinsulinism
Adipocytes
Formaldehyde
Glucocorticoids
Insulin Resistance
Microscopy
Cardiovascular Diseases
Therapeutics
Obesity
Antioxidants
Light

Keywords

  • Dexamethasone
  • Epicardial fat
  • Piper sarmentosum

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Siong, T. B., Ahmad, M. A. B., Isa, S. N. B. B., Jores, D. A., Kadir, H. S. B. A., Pin, P. Y., ... Suhaimi, F. (2010). The effect of Piper sarmentosum on the epicardial fat of the dexamethasone treated Sprague-Dawley rats. International Medical Journal, 17(3), 223-227.

The effect of Piper sarmentosum on the epicardial fat of the dexamethasone treated Sprague-Dawley rats. / Siong, Tioh Beng; Ahmad, Muhammad Aizuddin Bin; Isa, Siti Nor Badriah Binti; Jores, Drend Anak; Kadir, Hasni Syamila Binti Abdul; Pin, Phoong Yee; Othman, Faizah; Suhaimi, Farihah.

In: International Medical Journal, Vol. 17, No. 3, 09.2010, p. 223-227.

Research output: Contribution to journalArticle

Siong, TB, Ahmad, MAB, Isa, SNBB, Jores, DA, Kadir, HSBA, Pin, PY, Othman, F & Suhaimi, F 2010, 'The effect of Piper sarmentosum on the epicardial fat of the dexamethasone treated Sprague-Dawley rats', International Medical Journal, vol. 17, no. 3, pp. 223-227.
Siong TB, Ahmad MAB, Isa SNBB, Jores DA, Kadir HSBA, Pin PY et al. The effect of Piper sarmentosum on the epicardial fat of the dexamethasone treated Sprague-Dawley rats. International Medical Journal. 2010 Sep;17(3):223-227.
Siong, Tioh Beng ; Ahmad, Muhammad Aizuddin Bin ; Isa, Siti Nor Badriah Binti ; Jores, Drend Anak ; Kadir, Hasni Syamila Binti Abdul ; Pin, Phoong Yee ; Othman, Faizah ; Suhaimi, Farihah. / The effect of Piper sarmentosum on the epicardial fat of the dexamethasone treated Sprague-Dawley rats. In: International Medical Journal. 2010 ; Vol. 17, No. 3. pp. 223-227.
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abstract = "Objective: Medical treatments using dexamethasone exposes the body to a higher level of glucocorticoid, which then causes increased body adiposity, hyperinsulinemia and insulin resistance. It leads to obesity and further increases the epicardial fat thickness. This {"}ectopic fat storage syndrome{"} in the heart will subsequently contribute to the development of cardiovascular diseases. Piper sarmentosum, locally known as Kaduk is a traditional herb rich in natural antioxidants but its effectiveness of inhibiting the {"}ectopic fat storage syndrome{"} has not been studied. Therefore, it is our interest to determine the effect of Piper sarmentosum on the histological changes and histomorphometry in the architecture of the epicardial fat of the dexamethasone treated male adrenalectomized rats. Methods: Twenty-four male Sprague-Dawley rats, (2 months old) were divided into Baseline (B), Sham-operated (S), Adrenalectomized Control (C) and Adrenalectomized Piper sarmentosum (K) groups. Only the C and K groups received daily treatment of intramuscular dexamethasone (120 μg/kg/day) for a period of two months. Group K also received Piper sarmentosum extract (0.125 g/kg/day) during the same period. The animals were sacrificed and the heart tissues were taken and fixed in 10{\%} formalin for light microscopy. Results: Results demonstrated that the epicardial fat thickness (p < 0.05) and adipocytes number (p < 0.05) in K were significantly reduced when compared with C. Conclusion: Thus, these results imply that there is a potential role for Piper sarmentosum in inhibiting or delaying the {"}ectopic fat storage syndrome{"} in dexamethasone treatment.",
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AU - Jores, Drend Anak

AU - Kadir, Hasni Syamila Binti Abdul

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AB - Objective: Medical treatments using dexamethasone exposes the body to a higher level of glucocorticoid, which then causes increased body adiposity, hyperinsulinemia and insulin resistance. It leads to obesity and further increases the epicardial fat thickness. This "ectopic fat storage syndrome" in the heart will subsequently contribute to the development of cardiovascular diseases. Piper sarmentosum, locally known as Kaduk is a traditional herb rich in natural antioxidants but its effectiveness of inhibiting the "ectopic fat storage syndrome" has not been studied. Therefore, it is our interest to determine the effect of Piper sarmentosum on the histological changes and histomorphometry in the architecture of the epicardial fat of the dexamethasone treated male adrenalectomized rats. Methods: Twenty-four male Sprague-Dawley rats, (2 months old) were divided into Baseline (B), Sham-operated (S), Adrenalectomized Control (C) and Adrenalectomized Piper sarmentosum (K) groups. Only the C and K groups received daily treatment of intramuscular dexamethasone (120 μg/kg/day) for a period of two months. Group K also received Piper sarmentosum extract (0.125 g/kg/day) during the same period. The animals were sacrificed and the heart tissues were taken and fixed in 10% formalin for light microscopy. Results: Results demonstrated that the epicardial fat thickness (p < 0.05) and adipocytes number (p < 0.05) in K were significantly reduced when compared with C. Conclusion: Thus, these results imply that there is a potential role for Piper sarmentosum in inhibiting or delaying the "ectopic fat storage syndrome" in dexamethasone treatment.

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