The effect of Piper betel extract on the wound healing process in experimentally induced diabetic rats

E. C. Keat, S. S. Razak, N. M. Fadil, F. M. Yusof, L. H. Chah, F. K. Chyi, Seong Lin Teoh, Srijit Das, A. A. Latiff, M. Mazlan

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Abstract

Background. Piper betel (PB) possesses antimicrobial, antifungal, antioxidant and wound healing properties due to its powerful antioxidant effect. Diabetes mellitus (DM) is a metabolic disorder which is associated with complications like impaired wound healing, nephropathy and neuropathy. The main aim of the study was to study the wound healing properties of PB. Materials and Methods. A total of 33 male Sprague-Dawley rats (250-300g) were taken and divided into 3 groups:- Group I (control) comprising of 14 rats; Group II (diabetic untreated) comprising of 9 rats; Group III (diabetic treated) comprising of 10 rats. After 10 days of acclimatization, the animals were fasted overnight and diabetes was induced by administration of streptozotocin (45 mg/Kg body weight in a single dose, through tail vein) to group II and III animals. Four 6 mm-diameter full thickness skin excision wounds were created and PB extract (50 mg diluted in 0.1 ml of normal saline) was applied locally for 10 days in group III. The group I and II received normal saline (0.1 ml) for 10 days. The total protein content and the wound contraction rate were determined. Results. The wound contraction rate of group III (35.03 ± 2.96) was higher as compared to group II (18.40 ± 3.87) with p = 0.014. The total protein content for group III was 106.39 ± 4.46 as compared to group II (72.86 ± 12.86) with p = 0.050. Conclusion. PB acted as a protective agent in the early phase of wound healing by increasing total protein content and wound contraction rate.

Original languageEnglish
Pages (from-to)117-120
Number of pages4
JournalClinica Terapeutica
Volume161
Issue number2
Publication statusPublished - Mar 2010

Fingerprint

Piper
Wound Healing
Wounds and Injuries
Antioxidants
Protective Agents
Proteins
Acclimatization
Streptozocin
Sprague Dawley Rats
Tail
Veins
Diabetes Mellitus
Body Weight
Control Groups
Skin

Keywords

  • Antioxidant
  • Diabetes mellitus
  • Extract
  • Healing
  • Piper betel wound

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Keat, E. C., Razak, S. S., Fadil, N. M., Yusof, F. M., Chah, L. H., Chyi, F. K., ... Mazlan, M. (2010). The effect of Piper betel extract on the wound healing process in experimentally induced diabetic rats. Clinica Terapeutica, 161(2), 117-120.

The effect of Piper betel extract on the wound healing process in experimentally induced diabetic rats. / Keat, E. C.; Razak, S. S.; Fadil, N. M.; Yusof, F. M.; Chah, L. H.; Chyi, F. K.; Teoh, Seong Lin; Das, Srijit; Latiff, A. A.; Mazlan, M.

In: Clinica Terapeutica, Vol. 161, No. 2, 03.2010, p. 117-120.

Research output: Contribution to journalArticle

Keat, EC, Razak, SS, Fadil, NM, Yusof, FM, Chah, LH, Chyi, FK, Teoh, SL, Das, S, Latiff, AA & Mazlan, M 2010, 'The effect of Piper betel extract on the wound healing process in experimentally induced diabetic rats', Clinica Terapeutica, vol. 161, no. 2, pp. 117-120.
Keat EC, Razak SS, Fadil NM, Yusof FM, Chah LH, Chyi FK et al. The effect of Piper betel extract on the wound healing process in experimentally induced diabetic rats. Clinica Terapeutica. 2010 Mar;161(2):117-120.
Keat, E. C. ; Razak, S. S. ; Fadil, N. M. ; Yusof, F. M. ; Chah, L. H. ; Chyi, F. K. ; Teoh, Seong Lin ; Das, Srijit ; Latiff, A. A. ; Mazlan, M. / The effect of Piper betel extract on the wound healing process in experimentally induced diabetic rats. In: Clinica Terapeutica. 2010 ; Vol. 161, No. 2. pp. 117-120.
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abstract = "Background. Piper betel (PB) possesses antimicrobial, antifungal, antioxidant and wound healing properties due to its powerful antioxidant effect. Diabetes mellitus (DM) is a metabolic disorder which is associated with complications like impaired wound healing, nephropathy and neuropathy. The main aim of the study was to study the wound healing properties of PB. Materials and Methods. A total of 33 male Sprague-Dawley rats (250-300g) were taken and divided into 3 groups:- Group I (control) comprising of 14 rats; Group II (diabetic untreated) comprising of 9 rats; Group III (diabetic treated) comprising of 10 rats. After 10 days of acclimatization, the animals were fasted overnight and diabetes was induced by administration of streptozotocin (45 mg/Kg body weight in a single dose, through tail vein) to group II and III animals. Four 6 mm-diameter full thickness skin excision wounds were created and PB extract (50 mg diluted in 0.1 ml of normal saline) was applied locally for 10 days in group III. The group I and II received normal saline (0.1 ml) for 10 days. The total protein content and the wound contraction rate were determined. Results. The wound contraction rate of group III (35.03 ± 2.96) was higher as compared to group II (18.40 ± 3.87) with p = 0.014. The total protein content for group III was 106.39 ± 4.46 as compared to group II (72.86 ± 12.86) with p = 0.050. Conclusion. PB acted as a protective agent in the early phase of wound healing by increasing total protein content and wound contraction rate.",
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AU - Keat, E. C.

AU - Razak, S. S.

AU - Fadil, N. M.

AU - Yusof, F. M.

AU - Chah, L. H.

AU - Chyi, F. K.

AU - Teoh, Seong Lin

AU - Das, Srijit

AU - Latiff, A. A.

AU - Mazlan, M.

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N2 - Background. Piper betel (PB) possesses antimicrobial, antifungal, antioxidant and wound healing properties due to its powerful antioxidant effect. Diabetes mellitus (DM) is a metabolic disorder which is associated with complications like impaired wound healing, nephropathy and neuropathy. The main aim of the study was to study the wound healing properties of PB. Materials and Methods. A total of 33 male Sprague-Dawley rats (250-300g) were taken and divided into 3 groups:- Group I (control) comprising of 14 rats; Group II (diabetic untreated) comprising of 9 rats; Group III (diabetic treated) comprising of 10 rats. After 10 days of acclimatization, the animals were fasted overnight and diabetes was induced by administration of streptozotocin (45 mg/Kg body weight in a single dose, through tail vein) to group II and III animals. Four 6 mm-diameter full thickness skin excision wounds were created and PB extract (50 mg diluted in 0.1 ml of normal saline) was applied locally for 10 days in group III. The group I and II received normal saline (0.1 ml) for 10 days. The total protein content and the wound contraction rate were determined. Results. The wound contraction rate of group III (35.03 ± 2.96) was higher as compared to group II (18.40 ± 3.87) with p = 0.014. The total protein content for group III was 106.39 ± 4.46 as compared to group II (72.86 ± 12.86) with p = 0.050. Conclusion. PB acted as a protective agent in the early phase of wound healing by increasing total protein content and wound contraction rate.

AB - Background. Piper betel (PB) possesses antimicrobial, antifungal, antioxidant and wound healing properties due to its powerful antioxidant effect. Diabetes mellitus (DM) is a metabolic disorder which is associated with complications like impaired wound healing, nephropathy and neuropathy. The main aim of the study was to study the wound healing properties of PB. Materials and Methods. A total of 33 male Sprague-Dawley rats (250-300g) were taken and divided into 3 groups:- Group I (control) comprising of 14 rats; Group II (diabetic untreated) comprising of 9 rats; Group III (diabetic treated) comprising of 10 rats. After 10 days of acclimatization, the animals were fasted overnight and diabetes was induced by administration of streptozotocin (45 mg/Kg body weight in a single dose, through tail vein) to group II and III animals. Four 6 mm-diameter full thickness skin excision wounds were created and PB extract (50 mg diluted in 0.1 ml of normal saline) was applied locally for 10 days in group III. The group I and II received normal saline (0.1 ml) for 10 days. The total protein content and the wound contraction rate were determined. Results. The wound contraction rate of group III (35.03 ± 2.96) was higher as compared to group II (18.40 ± 3.87) with p = 0.014. The total protein content for group III was 106.39 ± 4.46 as compared to group II (72.86 ± 12.86) with p = 0.050. Conclusion. PB acted as a protective agent in the early phase of wound healing by increasing total protein content and wound contraction rate.

KW - Antioxidant

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