The effect of pharmaceuticals and personal care products (PPCPs) on human embryonic kidney (HEK 293) cells proliferation

Hind M. Ewadh, Siti Rozaimah Sheikh Abdullah, Hassimi Abu Hasan, Nurina Anwar, Mufeed Jalil Ewadh

Research output: Contribution to journalArticle

Abstract

Background: Pharmaceuticals and personal care products (PPCPs) considered an environmental issue that needs to be managed,especially, PPCPs are not removed by conventional waste water treatment. Therefore, the aim of current study was to analyses the toxicity of PPCPs, as single and mixed exposures, on human embryonic (HEK 293) cells proliferation. Methods:The culture medium RPMI Medium 1640 was used for growing the HEK293 cells. The PPCPs stocks were prepared with Dimethyl Sulfoxide (DMSO) and diluted into three concentrations (minimum, middle and maximum) as the working stocks for the toxicity study. The cell proliferation was studied using hemocytometer test. Results: The human embryonic kidney (HEK293) cella were very sensitive to the effects of pharmaceuticals and personal care products (PPCPs). The highest reduction in cells proliferation (90%) was seen upon exposure to a mixture of the maximum concentrations of PPCPs (750µg/L for IBU and HHCB, 75µg/L for KET and 5µg/L for EE 2 ) used in current study. In addition, for exposure to a single PPCP, the results showed that the highest reduction in cells proliferation (46%) was seen upon exposure to the synthetic hormone EE 2 (5µg/L). Conclusion: The human embryonic kidney cells (HEK293) used in this study were verry sensitive to the toxic effects of pharmaceuticals and personal care products (PPCPs). The latter can reflect the human sensitivity to the daily exposure to the PPCPs.

Original languageEnglish
Pages (from-to)3228-3232
Number of pages5
JournalJournal of Pharmaceutical Sciences and Research
Volume10
Issue number12
Publication statusPublished - 1 Dec 2018

Fingerprint

Pharmaceutical Services
HEK293 Cells
Cell Proliferation
Kidney
Water Purification
Poisons
Waste Water
Dimethyl Sulfoxide
Culture Media
Hormones

Keywords

  • 17α-ethinylestradiol
  • Cell culture
  • Galaxolide
  • HEK293 cells
  • Ibuprofen
  • Ketoprofen
  • Ozonation
  • PPCPs

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

The effect of pharmaceuticals and personal care products (PPCPs) on human embryonic kidney (HEK 293) cells proliferation. / Ewadh, Hind M.; Sheikh Abdullah, Siti Rozaimah; Abu Hasan, Hassimi; Anwar, Nurina; Ewadh, Mufeed Jalil.

In: Journal of Pharmaceutical Sciences and Research, Vol. 10, No. 12, 01.12.2018, p. 3228-3232.

Research output: Contribution to journalArticle

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AU - Ewadh, Mufeed Jalil

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