The effect of multilayer gold nanoparticles on the electrochemical response of ammonium ion biosensor based on alanine dehydrogenase enzyme

Ling Ling Tan @ Chong, Musa Ahmad, Yook Heng Lee, Toh Chee Seng

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2 Citations (Scopus)

Abstract

The use of multilayer of gold nanoparticles (AuNPs) attached on gold electrode surface via thiol chemistry to fabricate an ammonium (NH 4 +) ion biosensor based on alanine dehydrogenase (AlaDH) was investigated. The approach of the study was based on construction of biosensor by direct deposition of AuNPs and 1,8-octanedithiol (C8-DT) onto the gold electrode surface. For the immobilisation of enzyme, 2-mercaptoethanol (2BME) was first covalently attached to AlaDH via esther bonding and then followed by chemically attached the 2BME-modified AlaDH (2BME-AlaDH) moiety onto the AuNPs electrode via the exposed thiol group of 2BME. The resulting biosensor response was examined by means of amperometry for the quantification of NH4 + ion. In the absence of enzyme attachment, the use of three layers of AuNPs was found to improve the electrochemistry of the gold electrode when compared with no AuNPs was coated. However, when more than three layers of AuNPs were coated, the electrode response deteriorated due to excessive deposition of C8-DT. When AlaDH was incoporated into the AuNPs modified electrode, a linear response to NH4 + ion over the concentration range of 0.1-0.5mM with a detection limit of 0.01mM was obtained. In the absence of AuNPs, the NH4 + ion biosensor did not exhibit any good linear response range although the current response was observed to be higher. This work demonstrated that the incorporation of AuNPs could lead to the detection of higher NH4 + ion concentration without the need of dilution for high NH4 + ion concentration samples with a rapid response time of <1min.

Original languageEnglish
Article number754171
JournalJournal of Sensors
Volume2011
DOIs
Publication statusPublished - 2011

Fingerprint

dehydrogenases
alanine
bioinstrumentation
Biosensors
enzymes
Multilayers
Enzymes
Gold
gold
Nanoparticles
nanoparticles
Electrodes
electrodes
Ions
ions
ion concentration
thiols
Electrochemistry
electrochemistry
immobilization

ASJC Scopus subject areas

  • Instrumentation
  • Electrical and Electronic Engineering
  • Control and Systems Engineering

Cite this

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title = "The effect of multilayer gold nanoparticles on the electrochemical response of ammonium ion biosensor based on alanine dehydrogenase enzyme",
abstract = "The use of multilayer of gold nanoparticles (AuNPs) attached on gold electrode surface via thiol chemistry to fabricate an ammonium (NH 4 +) ion biosensor based on alanine dehydrogenase (AlaDH) was investigated. The approach of the study was based on construction of biosensor by direct deposition of AuNPs and 1,8-octanedithiol (C8-DT) onto the gold electrode surface. For the immobilisation of enzyme, 2-mercaptoethanol (2BME) was first covalently attached to AlaDH via esther bonding and then followed by chemically attached the 2BME-modified AlaDH (2BME-AlaDH) moiety onto the AuNPs electrode via the exposed thiol group of 2BME. The resulting biosensor response was examined by means of amperometry for the quantification of NH4 + ion. In the absence of enzyme attachment, the use of three layers of AuNPs was found to improve the electrochemistry of the gold electrode when compared with no AuNPs was coated. However, when more than three layers of AuNPs were coated, the electrode response deteriorated due to excessive deposition of C8-DT. When AlaDH was incoporated into the AuNPs modified electrode, a linear response to NH4 + ion over the concentration range of 0.1-0.5mM with a detection limit of 0.01mM was obtained. In the absence of AuNPs, the NH4 + ion biosensor did not exhibit any good linear response range although the current response was observed to be higher. This work demonstrated that the incorporation of AuNPs could lead to the detection of higher NH4 + ion concentration without the need of dilution for high NH4 + ion concentration samples with a rapid response time of <1min.",
author = "{Tan @ Chong}, {Ling Ling} and Musa Ahmad and Lee, {Yook Heng} and {Chee Seng}, Toh",
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AU - Ahmad, Musa

AU - Lee, Yook Heng

AU - Chee Seng, Toh

PY - 2011

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N2 - The use of multilayer of gold nanoparticles (AuNPs) attached on gold electrode surface via thiol chemistry to fabricate an ammonium (NH 4 +) ion biosensor based on alanine dehydrogenase (AlaDH) was investigated. The approach of the study was based on construction of biosensor by direct deposition of AuNPs and 1,8-octanedithiol (C8-DT) onto the gold electrode surface. For the immobilisation of enzyme, 2-mercaptoethanol (2BME) was first covalently attached to AlaDH via esther bonding and then followed by chemically attached the 2BME-modified AlaDH (2BME-AlaDH) moiety onto the AuNPs electrode via the exposed thiol group of 2BME. The resulting biosensor response was examined by means of amperometry for the quantification of NH4 + ion. In the absence of enzyme attachment, the use of three layers of AuNPs was found to improve the electrochemistry of the gold electrode when compared with no AuNPs was coated. However, when more than three layers of AuNPs were coated, the electrode response deteriorated due to excessive deposition of C8-DT. When AlaDH was incoporated into the AuNPs modified electrode, a linear response to NH4 + ion over the concentration range of 0.1-0.5mM with a detection limit of 0.01mM was obtained. In the absence of AuNPs, the NH4 + ion biosensor did not exhibit any good linear response range although the current response was observed to be higher. This work demonstrated that the incorporation of AuNPs could lead to the detection of higher NH4 + ion concentration without the need of dilution for high NH4 + ion concentration samples with a rapid response time of <1min.

AB - The use of multilayer of gold nanoparticles (AuNPs) attached on gold electrode surface via thiol chemistry to fabricate an ammonium (NH 4 +) ion biosensor based on alanine dehydrogenase (AlaDH) was investigated. The approach of the study was based on construction of biosensor by direct deposition of AuNPs and 1,8-octanedithiol (C8-DT) onto the gold electrode surface. For the immobilisation of enzyme, 2-mercaptoethanol (2BME) was first covalently attached to AlaDH via esther bonding and then followed by chemically attached the 2BME-modified AlaDH (2BME-AlaDH) moiety onto the AuNPs electrode via the exposed thiol group of 2BME. The resulting biosensor response was examined by means of amperometry for the quantification of NH4 + ion. In the absence of enzyme attachment, the use of three layers of AuNPs was found to improve the electrochemistry of the gold electrode when compared with no AuNPs was coated. However, when more than three layers of AuNPs were coated, the electrode response deteriorated due to excessive deposition of C8-DT. When AlaDH was incoporated into the AuNPs modified electrode, a linear response to NH4 + ion over the concentration range of 0.1-0.5mM with a detection limit of 0.01mM was obtained. In the absence of AuNPs, the NH4 + ion biosensor did not exhibit any good linear response range although the current response was observed to be higher. This work demonstrated that the incorporation of AuNPs could lead to the detection of higher NH4 + ion concentration without the need of dilution for high NH4 + ion concentration samples with a rapid response time of <1min.

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