The effect of MRN complex and ATM kinase inhibitors on Zebrafish embryonic development

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Zebrafish is an ideal animal model to study developmental biology due to its transparent embryos and rapid development stages of embryogenesis. Here we investigate the role of DNA damage proteins, specifically Mre11/Rad50/NBN (MRN) complex and ataxia-Telangiectasia mutated (ATM) kinase during embryogenesis by inhibiting its function using specific MRN complex (Mirin) and ATM Kinase inhibitors (Ku60019 and Ku55933). Zebrafish embryos at midblastula transition (MBT) stage are treated with Mirin, Ku60019 and Ku55933. The embryonic development of the embryos was monitored at 24 hours-post fertilisation (hpf), 48 hpf and 72 hpf. We observed that at the lowest concentrations (3 μM of Mirin, 1.5 nM of Ku60019 and 3 nM of Ku55933), the inhibitors treated embryos have 100% survivability. However, with increasing inhibitor concentration, the survivability drops. Control or mock treatment of all embryos shows 100 % survivability rate. This study suggests that DNA damage repair proteins may be crucial for normal zebrafish embryo development and survival.

Original languageEnglish
Title of host publication2017 UKM FST Postgraduate Colloquium
Subtitle of host publicationProceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1940
ISBN (Electronic)9780735416321
DOIs
Publication statusPublished - 4 Apr 2018
Event2017 UKM FST Postgraduate Colloquium - Selangor, Malaysia
Duration: 12 Jul 201713 Jul 2017

Other

Other2017 UKM FST Postgraduate Colloquium
CountryMalaysia
CitySelangor
Period12/7/1713/7/17

Fingerprint

ataxia
embryos
inhibitors
fertilization
deoxyribonucleic acid
damage
proteins
animal models
biology
low concentrations

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Kumaran, M., & Sa`Ariwijaya, M. S. F. (2018). The effect of MRN complex and ATM kinase inhibitors on Zebrafish embryonic development. In 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium (Vol. 1940). [020074] American Institute of Physics Inc.. https://doi.org/10.1063/1.5027989

The effect of MRN complex and ATM kinase inhibitors on Zebrafish embryonic development. / Kumaran, Malina; Sa`Ariwijaya, Mohd Shazrul Fazry.

2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940 American Institute of Physics Inc., 2018. 020074.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kumaran, M & Sa`Ariwijaya, MSF 2018, The effect of MRN complex and ATM kinase inhibitors on Zebrafish embryonic development. in 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. vol. 1940, 020074, American Institute of Physics Inc., 2017 UKM FST Postgraduate Colloquium, Selangor, Malaysia, 12/7/17. https://doi.org/10.1063/1.5027989
Kumaran M, Sa`Ariwijaya MSF. The effect of MRN complex and ATM kinase inhibitors on Zebrafish embryonic development. In 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940. American Institute of Physics Inc. 2018. 020074 https://doi.org/10.1063/1.5027989
Kumaran, Malina ; Sa`Ariwijaya, Mohd Shazrul Fazry. / The effect of MRN complex and ATM kinase inhibitors on Zebrafish embryonic development. 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940 American Institute of Physics Inc., 2018.
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