Kesan penambahan glukosa dalam fermentasi aseton-butanol-etanol daripada efluen kilang kelapa sawit oleh Clostridium acetobutylicum NCIMB 619

Translated title of the contribution: The effect of glucose addition in acetone-butanol-ethanol fermentation from palm oil mill effluent by Clostridium acetobutylicum NCIMB 619

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1 Citation (Scopus)

Abstract

This study investigates the production of acetone, butanol and ethanol (ABE) in sterilized sludge of palm oil mill effluent (POME) with the addition of glucose by Clostridium acetobutylicum NCIMB 619. Glucose concentrations of 5 g/L, 10 g/L and 15 g/L were used with volume percentage of 3% (v/v) and 5% (v/v) for each concentration. The highest ABE production of 9.89 g/L was observed with the addition of 10 g/L glucose with volume percentage of 5% (v/v) compared to 0.49 g/L in the control batch, with highest ABE productivity and yield, YP/S of 0.14 g/L/h and 1.36 g ABE/g substrate, respectively. Providing culture with 15 g/L glucose with volume percentage of 5% (v/v) was found to reduce the ABE production with lower productivity and ABE yield. The lower production at higher sugar level might be due to sugar inhibition that occurred during the process.

Original languageMalay
Pages (from-to)213-220
Number of pages8
JournalMalaysian Journal of Analytical Sciences
Volume21
Issue number1
DOIs
Publication statusPublished - 1 Feb 2017

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Clostridium
Butanols
Acetone
Fermentation
Effluents
Ethanol
Glucose
Sugars
Productivity
Sewage sludge
palm oil
Substrates

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

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title = "Kesan penambahan glukosa dalam fermentasi aseton-butanol-etanol daripada efluen kilang kelapa sawit oleh Clostridium acetobutylicum NCIMB 619",
abstract = "This study investigates the production of acetone, butanol and ethanol (ABE) in sterilized sludge of palm oil mill effluent (POME) with the addition of glucose by Clostridium acetobutylicum NCIMB 619. Glucose concentrations of 5 g/L, 10 g/L and 15 g/L were used with volume percentage of 3{\%} (v/v) and 5{\%} (v/v) for each concentration. The highest ABE production of 9.89 g/L was observed with the addition of 10 g/L glucose with volume percentage of 5{\%} (v/v) compared to 0.49 g/L in the control batch, with highest ABE productivity and yield, YP/S of 0.14 g/L/h and 1.36 g ABE/g substrate, respectively. Providing culture with 15 g/L glucose with volume percentage of 5{\%} (v/v) was found to reduce the ABE production with lower productivity and ABE yield. The lower production at higher sugar level might be due to sugar inhibition that occurred during the process.",
keywords = "Acetone-butanol-ethanol fermentation, Clostridium acetobutylicum, Glucose addition, Palm oil mill effluent",
author = "Japar, {Azima Syafaini} and Takriff, {Mohd Sobri} and {Md Jahim}, Jamaliah and Kadhum, {Abdul Amir H.}",
year = "2017",
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language = "Malay",
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T1 - Kesan penambahan glukosa dalam fermentasi aseton-butanol-etanol daripada efluen kilang kelapa sawit oleh Clostridium acetobutylicum NCIMB 619

AU - Japar, Azima Syafaini

AU - Takriff, Mohd Sobri

AU - Md Jahim, Jamaliah

AU - Kadhum, Abdul Amir H.

PY - 2017/2/1

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N2 - This study investigates the production of acetone, butanol and ethanol (ABE) in sterilized sludge of palm oil mill effluent (POME) with the addition of glucose by Clostridium acetobutylicum NCIMB 619. Glucose concentrations of 5 g/L, 10 g/L and 15 g/L were used with volume percentage of 3% (v/v) and 5% (v/v) for each concentration. The highest ABE production of 9.89 g/L was observed with the addition of 10 g/L glucose with volume percentage of 5% (v/v) compared to 0.49 g/L in the control batch, with highest ABE productivity and yield, YP/S of 0.14 g/L/h and 1.36 g ABE/g substrate, respectively. Providing culture with 15 g/L glucose with volume percentage of 5% (v/v) was found to reduce the ABE production with lower productivity and ABE yield. The lower production at higher sugar level might be due to sugar inhibition that occurred during the process.

AB - This study investigates the production of acetone, butanol and ethanol (ABE) in sterilized sludge of palm oil mill effluent (POME) with the addition of glucose by Clostridium acetobutylicum NCIMB 619. Glucose concentrations of 5 g/L, 10 g/L and 15 g/L were used with volume percentage of 3% (v/v) and 5% (v/v) for each concentration. The highest ABE production of 9.89 g/L was observed with the addition of 10 g/L glucose with volume percentage of 5% (v/v) compared to 0.49 g/L in the control batch, with highest ABE productivity and yield, YP/S of 0.14 g/L/h and 1.36 g ABE/g substrate, respectively. Providing culture with 15 g/L glucose with volume percentage of 5% (v/v) was found to reduce the ABE production with lower productivity and ABE yield. The lower production at higher sugar level might be due to sugar inhibition that occurred during the process.

KW - Acetone-butanol-ethanol fermentation

KW - Clostridium acetobutylicum

KW - Glucose addition

KW - Palm oil mill effluent

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