The effect of Gelam (Melaleuca Cajuputi) honey on inflammatory mediators in periodontitis-induced Sprague-Dawley rats

S. A. Aziz, N. Hamzah, A. R. Fauzi, Yasmin Anum Mohd Yusof, N. Ibrahim, Mariati Abdul Rahman, N. Abdul Ghafar, Badiah Baharin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Periodontitis is an inflammatory condition of the tooth supporting structures that will lead to tooth loss. Research has proven that Gelam honey has an anti-inflammatory effect and potential for treating inflammatory condition. This study aimed to determine the effect of Gelam honey on the inflammatory mediators systemically and locally in Sprague-Dawley rats with periodontitis. Twenty-eight rats were randomly assigned into 4 groups; NLS (control treated with saline), NLH (control treated with Gelam honey, 3g/kg body weight), LS (periodontitis treated with saline), LH (periodontitis treated with Gelam honey, 3g/kg body weight). Periodontitis was induced by ligating the lower left first molar with 4/0 Black Silk Suture. On day 15, plasma and tissue samples were analysed using ELISA and histological staining. The LS group exhibited significantly higher levels of inflammatory cells and IL-1β in the tissues than other groups. No significant difference of plasma IL-1β levels was found between all of the groups. Gelam honey was able to reduce the level of IL-1β in diseased rats with a total reduction of 21.26% in plasma, 81.27% in immunohistochemical staining. In conclusion, Gelam honey was able to reduce the level of IL-1β in periodontitis rats. Therefore, Gelam honey has potential to be included in dental products especially for treating periodontal disease. Industrial relevance. Periodontitis is one of the major dental diseases in most of the population in the world. It is an infectious disease and produced an inflammatory condition. Gelam honey (Melaleuca Cajuputi) is one of the fresh honeys which are easily available in Malaysia and research has proven that Gelam honey has an anti-inflammatory effect. The present study demonstrated that Gelam honey able to reduce inflammatory cells and mediators namely IL-1β in the plasma and in the tissue samples from rats induced with Periodontitis. The results of this study showed that Gelam honey has the potential to be the remedy for treating periodontal disease and this study may be the stepping stone to further the usage of honey in dentistry.

Original languageEnglish
Pages (from-to)7-16
Number of pages10
JournalInternational Journal of Applied Research in Natural Products
Volume7
Issue number3
Publication statusPublished - 22 Nov 2014

Fingerprint

Melaleuca
Honey
Periodontitis
Sprague Dawley Rats
Interleukin-1
Periodontal Diseases
Anti-Inflammatory Agents
Body Weight
Staining and Labeling
Stomatognathic Diseases
Tooth Loss
Periodontium
Silk
Malaysia

Keywords

  • Honey
  • Inflammation
  • Inflammatory mediators
  • Periodontitis

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Complementary and alternative medicine

Cite this

The effect of Gelam (Melaleuca Cajuputi) honey on inflammatory mediators in periodontitis-induced Sprague-Dawley rats. / Aziz, S. A.; Hamzah, N.; Fauzi, A. R.; Mohd Yusof, Yasmin Anum; Ibrahim, N.; Abdul Rahman, Mariati; Abdul Ghafar, N.; Baharin, Badiah.

In: International Journal of Applied Research in Natural Products, Vol. 7, No. 3, 22.11.2014, p. 7-16.

Research output: Contribution to journalArticle

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abstract = "Periodontitis is an inflammatory condition of the tooth supporting structures that will lead to tooth loss. Research has proven that Gelam honey has an anti-inflammatory effect and potential for treating inflammatory condition. This study aimed to determine the effect of Gelam honey on the inflammatory mediators systemically and locally in Sprague-Dawley rats with periodontitis. Twenty-eight rats were randomly assigned into 4 groups; NLS (control treated with saline), NLH (control treated with Gelam honey, 3g/kg body weight), LS (periodontitis treated with saline), LH (periodontitis treated with Gelam honey, 3g/kg body weight). Periodontitis was induced by ligating the lower left first molar with 4/0 Black Silk Suture. On day 15, plasma and tissue samples were analysed using ELISA and histological staining. The LS group exhibited significantly higher levels of inflammatory cells and IL-1β in the tissues than other groups. No significant difference of plasma IL-1β levels was found between all of the groups. Gelam honey was able to reduce the level of IL-1β in diseased rats with a total reduction of 21.26{\%} in plasma, 81.27{\%} in immunohistochemical staining. In conclusion, Gelam honey was able to reduce the level of IL-1β in periodontitis rats. Therefore, Gelam honey has potential to be included in dental products especially for treating periodontal disease. Industrial relevance. Periodontitis is one of the major dental diseases in most of the population in the world. It is an infectious disease and produced an inflammatory condition. Gelam honey (Melaleuca Cajuputi) is one of the fresh honeys which are easily available in Malaysia and research has proven that Gelam honey has an anti-inflammatory effect. The present study demonstrated that Gelam honey able to reduce inflammatory cells and mediators namely IL-1β in the plasma and in the tissue samples from rats induced with Periodontitis. The results of this study showed that Gelam honey has the potential to be the remedy for treating periodontal disease and this study may be the stepping stone to further the usage of honey in dentistry.",
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AU - Mohd Yusof, Yasmin Anum

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