Kesan sinaran gama ke atas struktur kimia dan ciri permukaan dipalmitoilfosfatidilkolina (DPPC)

Translated title of the contribution: The effect of gamma irradiation on the chemical structure and surface characteristics of dipalmitoylphosphatidylcholine (DPPC)

Hur Munawar Kabir Mohd, Ainee Fatimah Ahmad, Faizal K.P. Kunchi Mohamed, Nursaidatul Syafadillah Kamaruzaman, Ng Wei Ling, Poh Roe Jin, Muhamad Samudi Yasir, Mohd Hafez Mohd Isa, Suria Ramli, Shahidan Radiman, Irman Abdul Rahman

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Dipalmitoylphosphatidylcholine (DPPC) is one of the most abundant lipids in the human body and carries out important physiological functions such as liver protection and fighting infections. This study was conducted to investigate the effect of ionising radiation on the chemical structure and surface characteristic of DPPC. Synthetic DPPC was dissolved in chloroform (1mg/ml) and irradiated with Cobalt-60 (dose range of 50 - 200 Gy). The change in surface characteristics due to gamma irradiation was determined by means of monolayer compression isotherms using a Langmuir trough. From the change in the isotherm features, the threshold dose that affected the surface characteristics of the DPPC monolayer was determined to be 60 Gy. With increasing dose value, the plateau feature in the DPPC isotherm became shorter and was shifted to higher surface pressures. Analysis using High Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS) suggested that gamma irradiation of DPPC destroyed the chemical structure of DPPC and produced two main radiolytic products, namely lisophosphatidylcholine, LPC (~495.3142 g/mol) and phosphatidic acid, PA (~718.916 g/mol) with an average percentage of LPC and PA of 23% and 74%, respectively.

Original languageUndefined/Unknown
Pages (from-to)454-460
Number of pages7
JournalMalaysian Journal of Analytical Sciences
Volume17
Issue number3
Publication statusPublished - 2013

Fingerprint

1,2-Dipalmitoylphosphatidylcholine
Irradiation
Isotherms
Monolayers
Phosphatidic Acids
Ionizing radiation
High performance liquid chromatography
Chloroform
Cobalt
Liver
Mass spectrometry
Compaction
Lipids

Keywords

  • Dipalmitoylphosphatidylcholine (DPPC)
  • Gamma radiation
  • High Performance Liquid-Chromatography (HPLC)
  • Langmuir monolayer
  • Mass-Spectrometer (MS)

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Kesan sinaran gama ke atas struktur kimia dan ciri permukaan dipalmitoilfosfatidilkolina (DPPC). / Mohd, Hur Munawar Kabir; Ahmad, Ainee Fatimah; K.P. Kunchi Mohamed, Faizal; Kamaruzaman, Nursaidatul Syafadillah; Ling, Ng Wei; Jin, Poh Roe; Yasir, Muhamad Samudi; Isa, Mohd Hafez Mohd; Ramli, Suria; Radiman, Shahidan; Abdul Rahman, Irman.

In: Malaysian Journal of Analytical Sciences, Vol. 17, No. 3, 2013, p. 454-460.

Research output: Contribution to journalArticle

Mohd, Hur Munawar Kabir ; Ahmad, Ainee Fatimah ; K.P. Kunchi Mohamed, Faizal ; Kamaruzaman, Nursaidatul Syafadillah ; Ling, Ng Wei ; Jin, Poh Roe ; Yasir, Muhamad Samudi ; Isa, Mohd Hafez Mohd ; Ramli, Suria ; Radiman, Shahidan ; Abdul Rahman, Irman. / Kesan sinaran gama ke atas struktur kimia dan ciri permukaan dipalmitoilfosfatidilkolina (DPPC). In: Malaysian Journal of Analytical Sciences. 2013 ; Vol. 17, No. 3. pp. 454-460.
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abstract = "Dipalmitoylphosphatidylcholine (DPPC) is one of the most abundant lipids in the human body and carries out important physiological functions such as liver protection and fighting infections. This study was conducted to investigate the effect of ionising radiation on the chemical structure and surface characteristic of DPPC. Synthetic DPPC was dissolved in chloroform (1mg/ml) and irradiated with Cobalt-60 (dose range of 50 - 200 Gy). The change in surface characteristics due to gamma irradiation was determined by means of monolayer compression isotherms using a Langmuir trough. From the change in the isotherm features, the threshold dose that affected the surface characteristics of the DPPC monolayer was determined to be 60 Gy. With increasing dose value, the plateau feature in the DPPC isotherm became shorter and was shifted to higher surface pressures. Analysis using High Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS) suggested that gamma irradiation of DPPC destroyed the chemical structure of DPPC and produced two main radiolytic products, namely lisophosphatidylcholine, LPC (~495.3142 g/mol) and phosphatidic acid, PA (~718.916 g/mol) with an average percentage of LPC and PA of 23{\%} and 74{\%}, respectively.",
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AU - Ahmad, Ainee Fatimah

AU - K.P. Kunchi Mohamed, Faizal

AU - Kamaruzaman, Nursaidatul Syafadillah

AU - Ling, Ng Wei

AU - Jin, Poh Roe

AU - Yasir, Muhamad Samudi

AU - Isa, Mohd Hafez Mohd

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AU - Radiman, Shahidan

AU - Abdul Rahman, Irman

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AB - Dipalmitoylphosphatidylcholine (DPPC) is one of the most abundant lipids in the human body and carries out important physiological functions such as liver protection and fighting infections. This study was conducted to investigate the effect of ionising radiation on the chemical structure and surface characteristic of DPPC. Synthetic DPPC was dissolved in chloroform (1mg/ml) and irradiated with Cobalt-60 (dose range of 50 - 200 Gy). The change in surface characteristics due to gamma irradiation was determined by means of monolayer compression isotherms using a Langmuir trough. From the change in the isotherm features, the threshold dose that affected the surface characteristics of the DPPC monolayer was determined to be 60 Gy. With increasing dose value, the plateau feature in the DPPC isotherm became shorter and was shifted to higher surface pressures. Analysis using High Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS) suggested that gamma irradiation of DPPC destroyed the chemical structure of DPPC and produced two main radiolytic products, namely lisophosphatidylcholine, LPC (~495.3142 g/mol) and phosphatidic acid, PA (~718.916 g/mol) with an average percentage of LPC and PA of 23% and 74%, respectively.

KW - Dipalmitoylphosphatidylcholine (DPPC)

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