The effect of different conventional methods of extraction on Marantodes pumila var. pumila leaves in relation to free radical scavenging activity

Noor Huda Abd Hamid, Jalifah Latip, Masturah Markom

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Marantodes pumila is one of the important herbs used in traditional medicine. However, there is no report on the optimum method for the preparation of M. pumila extract enriched with bioactive compounds. Therefore, this study aim to establish the suitable method in the preparation of M. pumila extracts enriched with antioxidative compounds. Different conventional extraction methods such as maceration, reflux and Soxhlet in solvents of different polarities i.e. ethanol, 50 % aqueous ethanol and water were employed to prepare extracts. Constituents of each extract were profiled using RP-HPLC followed by the free radical scavenging assay using 2,2 diphenyl-1-picrylhydrazyl (DPPH) to determine the antioxidant activity. Among all extraction methods, maceration in 50 % aqueous ethanol gave the highest total percentage yield (22.41 %) while all extraction methods were able to extract the marker compounds, gallic acid (GA) and methyl gallate (MGA). The free radical scavenging activity of extracts varied from IC50 11.93μg/mL (50 % aqueous ethanol via reflux) to 64.15μg/mL (water via maceration). All extracts showed better scavenging activity as compared to synthetic antioxidant, BHT. The variation in the IC50 values is expected due to the presence of different types and/or concentration of bioactive compounds in each extract. This study may provide a preliminary insight for the preparation of standardized extracts of M. pumila enriched with bioactive compounds.

Original languageEnglish
Title of host publication2017 UKM FST Postgraduate Colloquium
Subtitle of host publicationProceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1940
ISBN (Electronic)9780735416321
DOIs
Publication statusPublished - 4 Apr 2018
Event2017 UKM FST Postgraduate Colloquium - Selangor, Malaysia
Duration: 12 Jul 201713 Jul 2017

Other

Other2017 UKM FST Postgraduate Colloquium
CountryMalaysia
CitySelangor
Period12/7/1713/7/17

Fingerprint

scavenging
free radicals
leaves
ethyl alcohol
activity (biology)
antioxidants
preparation
gallates
medicine
markers
water
polarity
acids

Keywords

  • DPPH
  • HPLC
  • maceration
  • Marantodes pumila
  • reflux
  • Soxhlet

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Hamid, N. H. A., Latip, J., & Markom, M. (2018). The effect of different conventional methods of extraction on Marantodes pumila var. pumila leaves in relation to free radical scavenging activity. In 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium (Vol. 1940). [020094] American Institute of Physics Inc.. https://doi.org/10.1063/1.5028009

The effect of different conventional methods of extraction on Marantodes pumila var. pumila leaves in relation to free radical scavenging activity. / Hamid, Noor Huda Abd; Latip, Jalifah; Markom, Masturah.

2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940 American Institute of Physics Inc., 2018. 020094.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hamid, NHA, Latip, J & Markom, M 2018, The effect of different conventional methods of extraction on Marantodes pumila var. pumila leaves in relation to free radical scavenging activity. in 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. vol. 1940, 020094, American Institute of Physics Inc., 2017 UKM FST Postgraduate Colloquium, Selangor, Malaysia, 12/7/17. https://doi.org/10.1063/1.5028009
Hamid NHA, Latip J, Markom M. The effect of different conventional methods of extraction on Marantodes pumila var. pumila leaves in relation to free radical scavenging activity. In 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940. American Institute of Physics Inc. 2018. 020094 https://doi.org/10.1063/1.5028009
Hamid, Noor Huda Abd ; Latip, Jalifah ; Markom, Masturah. / The effect of different conventional methods of extraction on Marantodes pumila var. pumila leaves in relation to free radical scavenging activity. 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940 American Institute of Physics Inc., 2018.
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