The effect of aeration, agitation and light on biohydrogen production by Rhodobacter sphaeroides NCIMB 8253

S. Za'imah Syed Jaapar, Mohd. Sahaid Kalil, Nurina Anuar

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Photo fermentation is a biological process that can be applied for hydrogen production. The process is environmental friendly which is operated under mild conditions using renewable resources. In order to increase yield of H2 produced by Rhodobacter sphaeroides, some experimental factors that may enhance H2 production were studied. The effect of operating parameters including agitation, aeration and light on hydrogen production using R. sphaeroides NCIMB 8253 was investigated. Rhodobacter sphaeroides NCIMB 8253 was grown in 100 mL serum bottle containing growth medium with maliec acid as the sole organic carbon source. The cultures were incubated anaerobically at 30°C with tungsten lamp (100 W) as the light source (3.8 klux) and argon gas was purged for maintaining anaerobic condition. The results show that maximum hydrogen produced was higher (54.37 mL) in static culture with 69.98% of H2 in the total gas compared with shake culture (11.57 mL) with 57.86% of H2. By using static culture, H2 produced was five times higher compared with non-static in both aerobic and anaerobic condition. It was found that growth and H2 production with fluorescent lamp showed better results than growth and H2 production with tungsten light.

Original languageEnglish
Pages (from-to)1253-1259
Number of pages7
JournalPakistan Journal of Biological Sciences
Volume12
Issue number18
DOIs
Publication statusPublished - 2009

Fingerprint

Rhodobacter sphaeroides
hydrogen production
agitation
aeration
tungsten
anaerobic conditions
gases
renewable resources
aerobic conditions
bottles
hydrogen
culture media
fermentation
carbon
acids

Keywords

  • Anaerobic fermentation
  • Hydrogen produced
  • Photo fermentation
  • Rhodobacter sphaeroides

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

The effect of aeration, agitation and light on biohydrogen production by Rhodobacter sphaeroides NCIMB 8253. / Za'imah Syed Jaapar, S.; Kalil, Mohd. Sahaid; Anuar, Nurina.

In: Pakistan Journal of Biological Sciences, Vol. 12, No. 18, 2009, p. 1253-1259.

Research output: Contribution to journalArticle

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