The discrimination of d-tartrate positive and d-tartrate negative S. enterica subsp. enterica serovar Paratyphi B isolated in Malaysia by phenotypic and genotypic methods

Norazah Ahmad, Shirley Tang Gee Hoon, Mohamed Kamel Abdul Ghani, Koh Yin Tee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Serotyping is not sufficient to differentiate between Salmonella species that cause paratyphoid fever from the strains that cause milder gastroenteritis as these organisms share the same serotype Salmonella Paratyphi B (S. Paratyphi B). Strains causing paratyphoid fever do not ferment d-tartrate and this key feature was used in this study to determine the prevalence of these strains among the collection of S. Paratyphi B strains isolated from patients in Malaysia. A total of 105 isolates of S. Paratyphi B were discriminated into d-tartrate positive (dT +) and d-tartrate negative (dT -) variants by two lead acetate test protocols and multiplex PCR. The lead acetate test protocol 1 differed from protocol 2 by a lower inoculum size and different incubation conditions while the multiplex PCR utilized 2 sets of primers targeting the ATG start codon of the gene STM3356. Lead acetate protocol 1 discriminated 97.1% of the isolates as S. Paratyphi B dT + and 2.9% as dT - while test protocol 2 discriminated all the isolates as S. Paratyphi B dT +. The multiplex PCR test identified all 105 isolates as S. Paratyphi B dT + strains. The concordance of the lead acetate test relative to that of multiplex PCR was 97.7% and 100% for protocol 1 and 2 respectively. This study showed that S. Paratyphi B dT + CR was shown to be a simpler, more rapid and reliable method to discriminate S. Paratyphi B than the phenotypic lead acetate test.

Original languageEnglish
Pages (from-to)35-39
Number of pages5
JournalMalaysian Journal of Pathology
Volume34
Issue number1
Publication statusPublished - Jun 2012

Fingerprint

Salmonella paratyphi B
Malaysia
Multiplex Polymerase Chain Reaction
Paratyphoid Fever
Serotyping
Initiator Codon
Serogroup
tartaric acid
Gastroenteritis
Salmonella
lead acetate

Keywords

  • D-tartrate
  • Lead acetate test
  • Multiplex PCR
  • S. Paratyphi B

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Histology

Cite this

@article{55ea1069b7874669bfd34b312fc29b5f,
title = "The discrimination of d-tartrate positive and d-tartrate negative S. enterica subsp. enterica serovar Paratyphi B isolated in Malaysia by phenotypic and genotypic methods",
abstract = "Serotyping is not sufficient to differentiate between Salmonella species that cause paratyphoid fever from the strains that cause milder gastroenteritis as these organisms share the same serotype Salmonella Paratyphi B (S. Paratyphi B). Strains causing paratyphoid fever do not ferment d-tartrate and this key feature was used in this study to determine the prevalence of these strains among the collection of S. Paratyphi B strains isolated from patients in Malaysia. A total of 105 isolates of S. Paratyphi B were discriminated into d-tartrate positive (dT +) and d-tartrate negative (dT -) variants by two lead acetate test protocols and multiplex PCR. The lead acetate test protocol 1 differed from protocol 2 by a lower inoculum size and different incubation conditions while the multiplex PCR utilized 2 sets of primers targeting the ATG start codon of the gene STM3356. Lead acetate protocol 1 discriminated 97.1{\%} of the isolates as S. Paratyphi B dT + and 2.9{\%} as dT - while test protocol 2 discriminated all the isolates as S. Paratyphi B dT +. The multiplex PCR test identified all 105 isolates as S. Paratyphi B dT + strains. The concordance of the lead acetate test relative to that of multiplex PCR was 97.7{\%} and 100{\%} for protocol 1 and 2 respectively. This study showed that S. Paratyphi B dT + CR was shown to be a simpler, more rapid and reliable method to discriminate S. Paratyphi B than the phenotypic lead acetate test.",
keywords = "D-tartrate, Lead acetate test, Multiplex PCR, S. Paratyphi B",
author = "Norazah Ahmad and {Gee Hoon}, {Shirley Tang} and {Abdul Ghani}, {Mohamed Kamel} and Tee, {Koh Yin}",
year = "2012",
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TY - JOUR

T1 - The discrimination of d-tartrate positive and d-tartrate negative S. enterica subsp. enterica serovar Paratyphi B isolated in Malaysia by phenotypic and genotypic methods

AU - Ahmad, Norazah

AU - Gee Hoon, Shirley Tang

AU - Abdul Ghani, Mohamed Kamel

AU - Tee, Koh Yin

PY - 2012/6

Y1 - 2012/6

N2 - Serotyping is not sufficient to differentiate between Salmonella species that cause paratyphoid fever from the strains that cause milder gastroenteritis as these organisms share the same serotype Salmonella Paratyphi B (S. Paratyphi B). Strains causing paratyphoid fever do not ferment d-tartrate and this key feature was used in this study to determine the prevalence of these strains among the collection of S. Paratyphi B strains isolated from patients in Malaysia. A total of 105 isolates of S. Paratyphi B were discriminated into d-tartrate positive (dT +) and d-tartrate negative (dT -) variants by two lead acetate test protocols and multiplex PCR. The lead acetate test protocol 1 differed from protocol 2 by a lower inoculum size and different incubation conditions while the multiplex PCR utilized 2 sets of primers targeting the ATG start codon of the gene STM3356. Lead acetate protocol 1 discriminated 97.1% of the isolates as S. Paratyphi B dT + and 2.9% as dT - while test protocol 2 discriminated all the isolates as S. Paratyphi B dT +. The multiplex PCR test identified all 105 isolates as S. Paratyphi B dT + strains. The concordance of the lead acetate test relative to that of multiplex PCR was 97.7% and 100% for protocol 1 and 2 respectively. This study showed that S. Paratyphi B dT + CR was shown to be a simpler, more rapid and reliable method to discriminate S. Paratyphi B than the phenotypic lead acetate test.

AB - Serotyping is not sufficient to differentiate between Salmonella species that cause paratyphoid fever from the strains that cause milder gastroenteritis as these organisms share the same serotype Salmonella Paratyphi B (S. Paratyphi B). Strains causing paratyphoid fever do not ferment d-tartrate and this key feature was used in this study to determine the prevalence of these strains among the collection of S. Paratyphi B strains isolated from patients in Malaysia. A total of 105 isolates of S. Paratyphi B were discriminated into d-tartrate positive (dT +) and d-tartrate negative (dT -) variants by two lead acetate test protocols and multiplex PCR. The lead acetate test protocol 1 differed from protocol 2 by a lower inoculum size and different incubation conditions while the multiplex PCR utilized 2 sets of primers targeting the ATG start codon of the gene STM3356. Lead acetate protocol 1 discriminated 97.1% of the isolates as S. Paratyphi B dT + and 2.9% as dT - while test protocol 2 discriminated all the isolates as S. Paratyphi B dT +. The multiplex PCR test identified all 105 isolates as S. Paratyphi B dT + strains. The concordance of the lead acetate test relative to that of multiplex PCR was 97.7% and 100% for protocol 1 and 2 respectively. This study showed that S. Paratyphi B dT + CR was shown to be a simpler, more rapid and reliable method to discriminate S. Paratyphi B than the phenotypic lead acetate test.

KW - D-tartrate

KW - Lead acetate test

KW - Multiplex PCR

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M3 - Article

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JO - Malaysian Journal of Pathology

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