The co-expression of estrogen receptor beta (ERß) and smooth muscle actin in phyllodes tumour of breast

A tissue microarray study

Nurhayati H. Munawer, Siti Aishah Md Ali, Reena Rahayu Md Zain, Rohaizak Muhammad

Research output: Contribution to journalArticle

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Abstract

Proliferation of phyllodes tumour is mainly in the stromal that is considered as the neoplastic component of phyllodes tumour. Estrogen Receptor (ER) that plays an important role in breast neoplasm is also involved in progression of phyllodes tumour. ERß is a type of ER which was reported to be expressed in the breast stroma while the expression of smooth muscle actin (SMA) in the stroma may differentiate histological grade of phyllodes tumour. We compared the expression of ERß to SMA in stromal component of phyllodes tumour using tissue microarray (TMA) technique. A TMA was constructed on 77 cases of phyllodes tumour (46 benign, 17 boderline and 14 malignant) using 0.6 mm punch kit (Alphelys Plaisir, France) and immunohistochemical staining was done using ERß and SMA. Phyllodes tumour seen in women aged more than 40 year-old with benign phyllodes tumour showed the lowest median of age (p=0.017). ERß expression in stromal component increased with histological grade of the tumour. SMA was positive in 62.8, 41.2 and 57.1% of benign, borderline and malignant phyllodes tumour, respectively. Both ERß (p=0.024) and SMA were commonly expressed in women aged ≥40 years. We found a significant co-expression of ERß and SMA (p=0.047) and 65.5% of these cases occured in women aged more than 40 years-old. The high expression of SMA in stroma of benign phyllodes tumour may demonstrate the potential of proliferation of this tumour to become malignant. High expression of ERß of malignant phyllodes tumour and its significant association with SMA suggests that co-expression of these two markers may play a role in stromal tumorigenesis of phyllodes tumour.

Original languageEnglish
Pages (from-to)233-240
Number of pages8
JournalSains Malaysiana
Volume43
Issue number2
Publication statusPublished - Feb 2014

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Phyllodes Tumor
Estrogen Receptor beta
Smooth Muscle
Actins
Breast
Estrogen Receptors
France

Keywords

  • Erß
  • Phyllodes tumour
  • Sma
  • Tissue microarray

ASJC Scopus subject areas

  • General

Cite this

The co-expression of estrogen receptor beta (ERß) and smooth muscle actin in phyllodes tumour of breast : A tissue microarray study. / Munawer, Nurhayati H.; Md Ali, Siti Aishah; Md Zain, Reena Rahayu; Muhammad, Rohaizak.

In: Sains Malaysiana, Vol. 43, No. 2, 02.2014, p. 233-240.

Research output: Contribution to journalArticle

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abstract = "Proliferation of phyllodes tumour is mainly in the stromal that is considered as the neoplastic component of phyllodes tumour. Estrogen Receptor (ER) that plays an important role in breast neoplasm is also involved in progression of phyllodes tumour. ER{\ss} is a type of ER which was reported to be expressed in the breast stroma while the expression of smooth muscle actin (SMA) in the stroma may differentiate histological grade of phyllodes tumour. We compared the expression of ER{\ss} to SMA in stromal component of phyllodes tumour using tissue microarray (TMA) technique. A TMA was constructed on 77 cases of phyllodes tumour (46 benign, 17 boderline and 14 malignant) using 0.6 mm punch kit (Alphelys Plaisir, France) and immunohistochemical staining was done using ER{\ss} and SMA. Phyllodes tumour seen in women aged more than 40 year-old with benign phyllodes tumour showed the lowest median of age (p=0.017). ER{\ss} expression in stromal component increased with histological grade of the tumour. SMA was positive in 62.8, 41.2 and 57.1{\%} of benign, borderline and malignant phyllodes tumour, respectively. Both ER{\ss} (p=0.024) and SMA were commonly expressed in women aged ≥40 years. We found a significant co-expression of ER{\ss} and SMA (p=0.047) and 65.5{\%} of these cases occured in women aged more than 40 years-old. The high expression of SMA in stroma of benign phyllodes tumour may demonstrate the potential of proliferation of this tumour to become malignant. High expression of ER{\ss} of malignant phyllodes tumour and its significant association with SMA suggests that co-expression of these two markers may play a role in stromal tumorigenesis of phyllodes tumour.",
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