The changes of stemness biomarkers expression in human adipose-derived stem cells during long-term manipulation

Wan Kamarul Zaman Wan Safwani, Suzana Makpol, Somasundaram Sathapan, Chua Kien Hui

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

One of the advantages of human adipose-derived stem cells (ASCs) in regenerative medicine is that they can be harvested in abundance. However, the stemness biomarkers, which marked the safety and efficacy of ASCs in accordance with the good manufacturing practice guidelines, is not yet well established. This study was designed to investigate the effect of long-term culture on the stemness properties of ASCs using quantitative real-time polymerase chain reaction and flow cytometry. Results showed the growth rate of ASCs was at its peak when they reached P10 (population doubling; PD = 26) but started to decrease when they were expanded to P15 (PD = 36) and P20 (PD = 46). The ASCs can be culture expanded with minimal alteration in the stemness genes and cluster of differentiation (CD) markers expression up to P10. Expression level of Sox2, Nestin, and Nanog3 was significantly decreased at later passage. CD31, CD45, CD117, and human leukocyte antigen DR, DQ, and DP were lowly expressed at P5 and P10 but their expressions increased significantly at P15 or P20. The differentiation ability of ASCs (adipogenesis, osteogenesis, and neurogenesis) also decreased in long-term culture. Our findings suggested that P10 (PD = 26) should be the "cutoff point" for clinical usage because ASCs at passage 15 onward showed significant changes in the stemness genes, CD markers expression, and differentiation capability.

Original languageEnglish
Pages (from-to)261-270
Number of pages10
JournalBiotechnology and Applied Biochemistry
Volume58
Issue number4
DOIs
Publication statusPublished - Jul 2011

Fingerprint

Biomarkers
Stem cells
Stem Cells
CD Antigens
Multigene Family
Cell culture
Genes
Nestin
Adipogenesis
Regenerative Medicine
Flow cytometry
Polymerase chain reaction
Neurogenesis
Antigens
HLA Antigens
Practice Guidelines
Osteogenesis
Real-Time Polymerase Chain Reaction
Flow Cytometry
Safety

Keywords

  • adipose-derived stem cells
  • biomarker
  • culture expansion
  • flow cytometry
  • gene expression
  • human
  • stemness

ASJC Scopus subject areas

  • Drug Discovery
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering
  • Process Chemistry and Technology
  • Bioengineering
  • Molecular Medicine
  • Biotechnology

Cite this

The changes of stemness biomarkers expression in human adipose-derived stem cells during long-term manipulation. / Wan Safwani, Wan Kamarul Zaman; Makpol, Suzana; Sathapan, Somasundaram; Kien Hui, Chua.

In: Biotechnology and Applied Biochemistry, Vol. 58, No. 4, 07.2011, p. 261-270.

Research output: Contribution to journalArticle

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