The antiproliferative effect of plam oil gamma-tocotrienol on isoprenoid pathway of hepatoma cell line

A. J. Aida Juliana, J. Zaklah, M. T. Abdul Gapor, W. Z. Wan Ngah

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Objectives: Study was conducted to determine the mechanism of antiproliferative activity of gamma-tocotrienol in signal transduction of isoprenoid pathway and Ras protein expression in HepG2 cell line. Design and Methods: HepG2 and WRL-68 were cultured and exposed with different concentration of GTT to determine the IC50 and their antiproliferative effect by using MTS assay. The GTT uptake was determined by HPLC. The effect of prenylated side chain of GTT was determined by co-incubation of GTT with farnesol and assayed by using BrdU. The Ras protein expression was analyzed using Western blotting. Results: Results showed that the IC50 GTT for HepG2 was 170 μM and WRL-68 was 500 μM within 48 h and its uptake was 12 μM and 40 μM respectively. Co-incubation of GTT with trans, trans-farnesol increases cell proliferation and Ras protein expression. While treatment of GTT or farnesol alone reduce cell proliferation and Ras protein expression. Conclusions: GTT was toxic against HepG2 at a lower concentration. Its antiproliferative activity against HepG2 might be due to the action of its prenylated side chain that induce the conversion of FPP to farnesol and downregulates HMGCoA reductase activity. Reducation of FPP might reduced Ras protein prenylation.

    Original languageEnglish
    Pages (from-to)576-583
    Number of pages8
    JournalEuropean Journal of Scientific Research
    Volume18
    Issue number4
    Publication statusPublished - 2007

    Fingerprint

    gamma-tocotrienol
    farnesol
    ras Proteins
    Farnesol
    isoprenoid
    isoprenoids
    Terpenes
    hepatoma
    Hepatocellular Carcinoma
    Pathway
    Oils
    protein synthesis
    Cells
    cell lines
    Proteins
    Protein
    Cell Line
    oils
    protein
    Line

    Keywords

    • Farnesol
    • Isoprenoid pathway
    • Ras protein
    • γ-tocotrienols

    ASJC Scopus subject areas

    • General

    Cite this

    Aida Juliana, A. J., Zaklah, J., Abdul Gapor, M. T., & Wan Ngah, W. Z. (2007). The antiproliferative effect of plam oil gamma-tocotrienol on isoprenoid pathway of hepatoma cell line. European Journal of Scientific Research, 18(4), 576-583.

    The antiproliferative effect of plam oil gamma-tocotrienol on isoprenoid pathway of hepatoma cell line. / Aida Juliana, A. J.; Zaklah, J.; Abdul Gapor, M. T.; Wan Ngah, W. Z.

    In: European Journal of Scientific Research, Vol. 18, No. 4, 2007, p. 576-583.

    Research output: Contribution to journalArticle

    Aida Juliana, AJ, Zaklah, J, Abdul Gapor, MT & Wan Ngah, WZ 2007, 'The antiproliferative effect of plam oil gamma-tocotrienol on isoprenoid pathway of hepatoma cell line', European Journal of Scientific Research, vol. 18, no. 4, pp. 576-583.
    Aida Juliana, A. J. ; Zaklah, J. ; Abdul Gapor, M. T. ; Wan Ngah, W. Z. / The antiproliferative effect of plam oil gamma-tocotrienol on isoprenoid pathway of hepatoma cell line. In: European Journal of Scientific Research. 2007 ; Vol. 18, No. 4. pp. 576-583.
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    abstract = "Objectives: Study was conducted to determine the mechanism of antiproliferative activity of gamma-tocotrienol in signal transduction of isoprenoid pathway and Ras protein expression in HepG2 cell line. Design and Methods: HepG2 and WRL-68 were cultured and exposed with different concentration of GTT to determine the IC50 and their antiproliferative effect by using MTS assay. The GTT uptake was determined by HPLC. The effect of prenylated side chain of GTT was determined by co-incubation of GTT with farnesol and assayed by using BrdU. The Ras protein expression was analyzed using Western blotting. Results: Results showed that the IC50 GTT for HepG2 was 170 μM and WRL-68 was 500 μM within 48 h and its uptake was 12 μM and 40 μM respectively. Co-incubation of GTT with trans, trans-farnesol increases cell proliferation and Ras protein expression. While treatment of GTT or farnesol alone reduce cell proliferation and Ras protein expression. Conclusions: GTT was toxic against HepG2 at a lower concentration. Its antiproliferative activity against HepG2 might be due to the action of its prenylated side chain that induce the conversion of FPP to farnesol and downregulates HMGCoA reductase activity. Reducation of FPP might reduced Ras protein prenylation.",
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    AU - Wan Ngah, W. Z.

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