Tc99m-sestamibi: Pembangunan kit radiofarmaseutikal untuk pemetaan jantung

Translated title of the contribution: Tc99m-sestamibi: Development of radiopharmaceutical kit for heart imaging

W. K. Wan Hamirul Bahrin, Mohd Cairul Iqbal Mohd Amin, H. Rosnani, M. H. Zulkifli, T. Yahaya, A. Karuppaiya, A. Rasyieda

Research output: Contribution to journalArticle

Abstract

A development and comparison study of the Nuclear Malaysis's Tc 99m-sestamibi heart imaging agents (NM kits) and the commercial products is reported. Three batches of kits labelled as B1, B2 and B3 were produced by freeze drying technique. The commercial products such as sestamibi Cardiolite, Polatom, ChiMlBl and tetrofosmin Myoview were used in this study. The quality control testing which included microbiology testing, radiochemical and animal biodistribution study were conducted accordingly. The NM sestamibi kits passed the sterility and pirogen test. The Radiochemical Purity testing (RCP) was assessed by the Instant Thin Layer Chromatography (ITLC) and High-Performance Liquid Chromatography (HPLC) methods after the reconstitution of the Tc99m-sestamibi. The RCP results were above 90% and the kits were stable for 52 weeks. The animal biodistribution studies were carried out on Sprague-Dawley rats at 5. 30, 60, 120 and 1440 minutes post injection time intervals. The percentage injected dose per gram organ in heart for Tc 99m-B1 were 4.722±0.343%, 3.752±0.438%, 4.564±0.664%, 4.180±1.293% and 1.090±0.120% at 5, 30, 60, 120 and 1440 minutes respectively. This is followed by the Tc99m-B2; 3.852±0.406%, 3.268±0.425%, 3.366±0.316%, 4.324±1.044% and 1.038±0.144%, Tc99m-B3; 5.404±0.351%. 4.818pM0.579%, 6.015±0.774%, 5.028±1.353% and 1.623±0.692% at the same time intervals. Independent T Test showed that heart uptake was significant as compared to the control (p<0.05). The biodistribution study also showed that the radiopharmaceuticals localized selectively in the myocardium of rats. The NM sestamibi kits were comparable to the commercial products. Blood, lung, kidney and intestines washout were found to be fast and efficient. The biodistribution and analysis of heart-to-lung and heart-to-liver uptake ratios showed that NM sestamibi kits has a potential and could be used as heart imaging agents.

Original languageUndefined/Unknown
Pages (from-to)601-607
Number of pages7
JournalSains Malaysiana
Volume38
Issue number4
Publication statusPublished - Aug 2009

Fingerprint

Radiopharmaceuticals
Technetium Tc 99m Sestamibi
Lung
Freeze Drying
Thin Layer Chromatography
Microbiology
Quality Control
Infertility
Intestines
Sprague Dawley Rats
Myocardium
High Pressure Liquid Chromatography
Kidney
Injections
Liver

Keywords

  • Animal biodistribution study
  • Radiochemical purity
  • Radiopharmaceuticals
  • Tc-sestamibi

ASJC Scopus subject areas

  • General

Cite this

Wan Hamirul Bahrin, W. K., Mohd Amin, M. C. I., Rosnani, H., Zulkifli, M. H., Yahaya, T., Karuppaiya, A., & Rasyieda, A. (2009). Tc99m-sestamibi: Pembangunan kit radiofarmaseutikal untuk pemetaan jantung. Sains Malaysiana, 38(4), 601-607.

Tc99m-sestamibi : Pembangunan kit radiofarmaseutikal untuk pemetaan jantung. / Wan Hamirul Bahrin, W. K.; Mohd Amin, Mohd Cairul Iqbal; Rosnani, H.; Zulkifli, M. H.; Yahaya, T.; Karuppaiya, A.; Rasyieda, A.

In: Sains Malaysiana, Vol. 38, No. 4, 08.2009, p. 601-607.

Research output: Contribution to journalArticle

Wan Hamirul Bahrin, WK, Mohd Amin, MCI, Rosnani, H, Zulkifli, MH, Yahaya, T, Karuppaiya, A & Rasyieda, A 2009, 'Tc99m-sestamibi: Pembangunan kit radiofarmaseutikal untuk pemetaan jantung', Sains Malaysiana, vol. 38, no. 4, pp. 601-607.
Wan Hamirul Bahrin WK, Mohd Amin MCI, Rosnani H, Zulkifli MH, Yahaya T, Karuppaiya A et al. Tc99m-sestamibi: Pembangunan kit radiofarmaseutikal untuk pemetaan jantung. Sains Malaysiana. 2009 Aug;38(4):601-607.
Wan Hamirul Bahrin, W. K. ; Mohd Amin, Mohd Cairul Iqbal ; Rosnani, H. ; Zulkifli, M. H. ; Yahaya, T. ; Karuppaiya, A. ; Rasyieda, A. / Tc99m-sestamibi : Pembangunan kit radiofarmaseutikal untuk pemetaan jantung. In: Sains Malaysiana. 2009 ; Vol. 38, No. 4. pp. 601-607.
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