Systematic relationships of Oriental tiny frogs of the family Microhylidae (Amphibia, Anura) as revealed by mtDNA genealogy

Masafumi Matsui, Amir Hamidy, Daicus M. Belabut, Norhayati Ahmad, Somsak Panha, Ahmad Sudin, Wichase Khonsue, Hong Shik Oh, Hoi Sen Yong, Jian Ping Jiang, Kanto Nishikawa

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

We estimated the genealogical relationships and assessed systematic relationships among 45 out of 89 named species and four unnamed taxa from 11 of 14 genera of the Oriental microhylids from 1767. bp sequences of the mitochondrial DNA genes 12S rRNA and 16S rRNA using maximum parsimony, maximum likelihood, and Bayesian inference methods. Monophyly was rejected for the subfamily Microhylinae, and our data reveal four well-supported clades whose relationships to each other are unresolved: (A) Microhyla, Calluella, and Glyphoglossus, (B) Chaperina, (C) Kaloula, Phrynella, and Metaphrynella, and (D) Micryletta. They were genetically as divergent from each other as from another Oriental subfamily Kalophryninae, and could be recognized as distinct subfamilies. Within Clade A, our data reveal three well-supported subclades whose relationships to each other are unresolved: (AI) Microhyla-I, (AII) Calluella and Glyphoglossus, and (AIII) Microhyla-II. Of the two enigmatic Malaysian genera, whose subfamilial placement has been undetermined, Phrynella was found to be the sister species of Metaphrynella in Clade C, whereas Gastrophrynoides was grouped in the Papua-Australian subfamily Asterophryinae. Currently recognized subgenera and species groups within Microhyla based on morphology were not supported phylogenetically, and require thorough reassessments.

Original languageEnglish
Pages (from-to)167-176
Number of pages10
JournalMolecular Phylogenetics and Evolution
Volume61
Issue number1
DOIs
Publication statusPublished - Oct 2011

Fingerprint

Genealogy and Heraldry
genealogy
Mitochondrial Genes
Bayes Theorem
Amphibia
Amphibians
Anura
Mitochondrial DNA
frog
frogs
mitochondrial DNA
ribosomal RNA
Bayesian theory
monophyly
genes
gene
ribosomal RNA 12S
Microhylidae
family

Keywords

  • Asia
  • Gastrophrynoides
  • MtDNA
  • Papua-Australian element
  • Phrynella
  • Subgeneric classification

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

Cite this

Systematic relationships of Oriental tiny frogs of the family Microhylidae (Amphibia, Anura) as revealed by mtDNA genealogy. / Matsui, Masafumi; Hamidy, Amir; Belabut, Daicus M.; Ahmad, Norhayati; Panha, Somsak; Sudin, Ahmad; Khonsue, Wichase; Oh, Hong Shik; Yong, Hoi Sen; Jiang, Jian Ping; Nishikawa, Kanto.

In: Molecular Phylogenetics and Evolution, Vol. 61, No. 1, 10.2011, p. 167-176.

Research output: Contribution to journalArticle

Matsui, M, Hamidy, A, Belabut, DM, Ahmad, N, Panha, S, Sudin, A, Khonsue, W, Oh, HS, Yong, HS, Jiang, JP & Nishikawa, K 2011, 'Systematic relationships of Oriental tiny frogs of the family Microhylidae (Amphibia, Anura) as revealed by mtDNA genealogy', Molecular Phylogenetics and Evolution, vol. 61, no. 1, pp. 167-176. https://doi.org/10.1016/j.ympev.2011.05.015
Matsui, Masafumi ; Hamidy, Amir ; Belabut, Daicus M. ; Ahmad, Norhayati ; Panha, Somsak ; Sudin, Ahmad ; Khonsue, Wichase ; Oh, Hong Shik ; Yong, Hoi Sen ; Jiang, Jian Ping ; Nishikawa, Kanto. / Systematic relationships of Oriental tiny frogs of the family Microhylidae (Amphibia, Anura) as revealed by mtDNA genealogy. In: Molecular Phylogenetics and Evolution. 2011 ; Vol. 61, No. 1. pp. 167-176.
@article{a2d1153682d04aa1b73ec390321593af,
title = "Systematic relationships of Oriental tiny frogs of the family Microhylidae (Amphibia, Anura) as revealed by mtDNA genealogy",
abstract = "We estimated the genealogical relationships and assessed systematic relationships among 45 out of 89 named species and four unnamed taxa from 11 of 14 genera of the Oriental microhylids from 1767. bp sequences of the mitochondrial DNA genes 12S rRNA and 16S rRNA using maximum parsimony, maximum likelihood, and Bayesian inference methods. Monophyly was rejected for the subfamily Microhylinae, and our data reveal four well-supported clades whose relationships to each other are unresolved: (A) Microhyla, Calluella, and Glyphoglossus, (B) Chaperina, (C) Kaloula, Phrynella, and Metaphrynella, and (D) Micryletta. They were genetically as divergent from each other as from another Oriental subfamily Kalophryninae, and could be recognized as distinct subfamilies. Within Clade A, our data reveal three well-supported subclades whose relationships to each other are unresolved: (AI) Microhyla-I, (AII) Calluella and Glyphoglossus, and (AIII) Microhyla-II. Of the two enigmatic Malaysian genera, whose subfamilial placement has been undetermined, Phrynella was found to be the sister species of Metaphrynella in Clade C, whereas Gastrophrynoides was grouped in the Papua-Australian subfamily Asterophryinae. Currently recognized subgenera and species groups within Microhyla based on morphology were not supported phylogenetically, and require thorough reassessments.",
keywords = "Asia, Gastrophrynoides, MtDNA, Papua-Australian element, Phrynella, Subgeneric classification",
author = "Masafumi Matsui and Amir Hamidy and Belabut, {Daicus M.} and Norhayati Ahmad and Somsak Panha and Ahmad Sudin and Wichase Khonsue and Oh, {Hong Shik} and Yong, {Hoi Sen} and Jiang, {Jian Ping} and Kanto Nishikawa",
year = "2011",
month = "10",
doi = "10.1016/j.ympev.2011.05.015",
language = "English",
volume = "61",
pages = "167--176",
journal = "Molecular Phylogenetics and Evolution",
issn = "1055-7903",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Systematic relationships of Oriental tiny frogs of the family Microhylidae (Amphibia, Anura) as revealed by mtDNA genealogy

AU - Matsui, Masafumi

AU - Hamidy, Amir

AU - Belabut, Daicus M.

AU - Ahmad, Norhayati

AU - Panha, Somsak

AU - Sudin, Ahmad

AU - Khonsue, Wichase

AU - Oh, Hong Shik

AU - Yong, Hoi Sen

AU - Jiang, Jian Ping

AU - Nishikawa, Kanto

PY - 2011/10

Y1 - 2011/10

N2 - We estimated the genealogical relationships and assessed systematic relationships among 45 out of 89 named species and four unnamed taxa from 11 of 14 genera of the Oriental microhylids from 1767. bp sequences of the mitochondrial DNA genes 12S rRNA and 16S rRNA using maximum parsimony, maximum likelihood, and Bayesian inference methods. Monophyly was rejected for the subfamily Microhylinae, and our data reveal four well-supported clades whose relationships to each other are unresolved: (A) Microhyla, Calluella, and Glyphoglossus, (B) Chaperina, (C) Kaloula, Phrynella, and Metaphrynella, and (D) Micryletta. They were genetically as divergent from each other as from another Oriental subfamily Kalophryninae, and could be recognized as distinct subfamilies. Within Clade A, our data reveal three well-supported subclades whose relationships to each other are unresolved: (AI) Microhyla-I, (AII) Calluella and Glyphoglossus, and (AIII) Microhyla-II. Of the two enigmatic Malaysian genera, whose subfamilial placement has been undetermined, Phrynella was found to be the sister species of Metaphrynella in Clade C, whereas Gastrophrynoides was grouped in the Papua-Australian subfamily Asterophryinae. Currently recognized subgenera and species groups within Microhyla based on morphology were not supported phylogenetically, and require thorough reassessments.

AB - We estimated the genealogical relationships and assessed systematic relationships among 45 out of 89 named species and four unnamed taxa from 11 of 14 genera of the Oriental microhylids from 1767. bp sequences of the mitochondrial DNA genes 12S rRNA and 16S rRNA using maximum parsimony, maximum likelihood, and Bayesian inference methods. Monophyly was rejected for the subfamily Microhylinae, and our data reveal four well-supported clades whose relationships to each other are unresolved: (A) Microhyla, Calluella, and Glyphoglossus, (B) Chaperina, (C) Kaloula, Phrynella, and Metaphrynella, and (D) Micryletta. They were genetically as divergent from each other as from another Oriental subfamily Kalophryninae, and could be recognized as distinct subfamilies. Within Clade A, our data reveal three well-supported subclades whose relationships to each other are unresolved: (AI) Microhyla-I, (AII) Calluella and Glyphoglossus, and (AIII) Microhyla-II. Of the two enigmatic Malaysian genera, whose subfamilial placement has been undetermined, Phrynella was found to be the sister species of Metaphrynella in Clade C, whereas Gastrophrynoides was grouped in the Papua-Australian subfamily Asterophryinae. Currently recognized subgenera and species groups within Microhyla based on morphology were not supported phylogenetically, and require thorough reassessments.

KW - Asia

KW - Gastrophrynoides

KW - MtDNA

KW - Papua-Australian element

KW - Phrynella

KW - Subgeneric classification

UR - http://www.scopus.com/inward/record.url?scp=79960595654&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960595654&partnerID=8YFLogxK

U2 - 10.1016/j.ympev.2011.05.015

DO - 10.1016/j.ympev.2011.05.015

M3 - Article

C2 - 21658458

AN - SCOPUS:79960595654

VL - 61

SP - 167

EP - 176

JO - Molecular Phylogenetics and Evolution

JF - Molecular Phylogenetics and Evolution

SN - 1055-7903

IS - 1

ER -