Synthesis of polystyrene microspheres and functionalization with Pd(0) nanoparticles to perform bioorthogonal organometallic chemistry in living cells.

Asier Unciti-Broceta, Emma M V Johansson, Muhammad Rahimi Usop, Rosario M. Sánchez-Martín, Mark Bradley

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

We have developed miniaturized heterogeneous Pd(0)-catalysts (Pd(0)-microspheres) with the ability to enter cells, stay harmlessly within the cytosol and mediate efficient bioorthogonal organometallic chemistries (e.g., allylcarbamate cleavage and Suzuki-Miyaura cross-coupling). This approach is a major addition to the toolbox available for performing chemical reactions within cells. Here we describe a full protocol for the synthesis of the Pd(0)-microspheres from readily available starting materials (by the synthesis of size-controlled amino-functionalized polystyrene microspheres), as well as for their characterization (electron microscopy and palladium quantitation) and functional validation ('in solution' and 'in cytoplasm' conversions). From the beginning of the synthesis to functional evaluation of the catalytic device requires 5 d of work.

Original languageEnglish
Pages (from-to)1207-1218
Number of pages12
JournalNature Protocols
Volume7
Issue number6
Publication statusPublished - 2012

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Polystyrenes
Organometallics
Microspheres
Nanoparticles
Cells
Palladium
Cytosol
Electron microscopy
Chemical reactions
Electron Microscopy
Cytoplasm
Equipment and Supplies
Catalysts

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Synthesis of polystyrene microspheres and functionalization with Pd(0) nanoparticles to perform bioorthogonal organometallic chemistry in living cells. / Unciti-Broceta, Asier; Johansson, Emma M V; Usop, Muhammad Rahimi; Sánchez-Martín, Rosario M.; Bradley, Mark.

In: Nature Protocols, Vol. 7, No. 6, 2012, p. 1207-1218.

Research output: Contribution to journalArticle

Unciti-Broceta, Asier ; Johansson, Emma M V ; Usop, Muhammad Rahimi ; Sánchez-Martín, Rosario M. ; Bradley, Mark. / Synthesis of polystyrene microspheres and functionalization with Pd(0) nanoparticles to perform bioorthogonal organometallic chemistry in living cells. In: Nature Protocols. 2012 ; Vol. 7, No. 6. pp. 1207-1218.
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