Synthesis of a new potential conjugated TAT-peptide-chitosan nanoparticles carrier via disulphide linkage

Haliza Katas, Nik Nur Shamiha Nik Dzulkefli, Shariza Sahudin

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Chitosan and TAT peptide have been widely investigated as delivery systems for various biomolecules such as plasmid DNA, oligonucleotides, and siRNAs. Conjugation of chitosan with TAT-peptide was therefore expected to produce a carrier with enhanced ability to facilitate cellular uptake. In this study, chitosan nanoparticles (CNs) were prepared by ionic gelation method prior to conjugation with TAT-peptide via disulphide linkage (CN-TAT). The conjugation was performed at various TAT-peptide-to-chitosan weight ratios ranging from 0.008:1 to 0.125:1. siRNA as a model biomolecule was loaded by adsorption onto the CN-TAT. Nanosize range particles were produced with a size range of less than 700nm depending on TAT-peptide concentration used. HPLC and Raman spectrometry analysis revealed that TAT-peptide was successfully conjugated to the CN via disulphide linkage. siRNA loading efficiency for CN-TAT was 93 ± 0.01. In vitro cytotoxicity studies showed that CN-TAT has relatively low toxicity. In conclusion, TAT conjugated onto CN via disulphide linkage was successfully synthesized, and its low cytoxicity demonstrates a potential for its use as a vector for biomolecules.

Original languageEnglish
Article number134607
JournalJournal of Nanomaterials
Volume2012
DOIs
Publication statusPublished - 2012

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Chitosan
Disulfides
Peptides
Nanoparticles
Biomolecules
Small Interfering RNA
Oligonucleotides
Gelation
Cytotoxicity
Spectrometry
Toxicity
Plasmids
DNA
Adsorption

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Synthesis of a new potential conjugated TAT-peptide-chitosan nanoparticles carrier via disulphide linkage. / Katas, Haliza; Nik Dzulkefli, Nik Nur Shamiha; Sahudin, Shariza.

In: Journal of Nanomaterials, Vol. 2012, 134607, 2012.

Research output: Contribution to journalArticle

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