Synergistic effects on process parameters to enhance enzymatic hydrolysis of alkaline oil palm fronds

Masniroszaime Md Zain, Abdul Wahab Mohammad, Shuhaida Harun, Nurul Aina Fauzi, Nur Hanis Hayati Hairom

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Due to an increasing demand for more sustainable and renewable resources, there has been strong interest in utilizing biomass as a source for cleaner production of energy and chemicals. In this work, the lignocellulosic elements of oil palm frond (OPF) biomass were assessed as an alternate sugar feedstock for biofuel and bioproducts production. At present, long hydrolysis times and high enzymatic loadings hinder commercialisation and large-scale utilisation of enzymatic hydrolysis of lignocellulosic biomass. Thus, various process parameters of enzymatic hydrolysis of alkaline OPF fibre were investigated in an attempt to improve process performance. In this study, OPF biomass was pretreated with 4.42% NaOH at 100 °C for 58.31 min, resulting in significant disruption as characterised by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM). Alkaline pretreatment of OPF biomass improved enzymatic biodegradability, and glucan recovery by the Cellic Ctec2 enzyme was more effective than the conventional Celluclast 1.5 L cellulase enzyme. Synergistic effects of stirring speed, surfactant Triton X-100 loading, and β-glucosidase supplement on enzymatic hydrolysis were assessed using statistical experimental design. Under optimal conditions (450 rpm, 1.31%, and 0.14 pNPGU/FPU), 88% conversion of glucan was obtained from alkaline OPF, which is equivalent to the conversion from commercial cellulose (microcrystalline cellulose, MCC). Enzymatic hydrolysis of pretreated OPF was further improved at high agitation speeds. Synergy between agitation speed and surfactant loading interactions with β-glucosidase supplement enhanced glucose production due to the efficient mixing and availability of cellulose to be adsorbed by cellulase.

Original languageEnglish
Pages (from-to)617-626
Number of pages10
JournalIndustrial Crops and Products
Volume122
DOIs
Publication statusPublished - 15 Oct 2018

Fingerprint

Elaeis guineensis
fronds
enzymatic hydrolysis
biomass
glucosidases
cellulose
agitation
glucans
surfactants
biobased products
renewable resources
biodegradability
cellulases
cleaners
Fourier transform infrared spectroscopy
commercialization
feedstocks
biofuels
endo-1,4-beta-glucanase
X-ray diffraction

Keywords

  • Cellulase
  • Enzyme hydrolysis
  • Glucose
  • Oil palm frond
  • Sodium hydroxide pretreatment

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Synergistic effects on process parameters to enhance enzymatic hydrolysis of alkaline oil palm fronds. / Zain, Masniroszaime Md; Mohammad, Abdul Wahab; Harun, Shuhaida; Fauzi, Nurul Aina; Hairom, Nur Hanis Hayati.

In: Industrial Crops and Products, Vol. 122, 15.10.2018, p. 617-626.

Research output: Contribution to journalArticle

@article{1a27057d5bd349ecbfb8eb1c3a42d87c,
title = "Synergistic effects on process parameters to enhance enzymatic hydrolysis of alkaline oil palm fronds",
abstract = "Due to an increasing demand for more sustainable and renewable resources, there has been strong interest in utilizing biomass as a source for cleaner production of energy and chemicals. In this work, the lignocellulosic elements of oil palm frond (OPF) biomass were assessed as an alternate sugar feedstock for biofuel and bioproducts production. At present, long hydrolysis times and high enzymatic loadings hinder commercialisation and large-scale utilisation of enzymatic hydrolysis of lignocellulosic biomass. Thus, various process parameters of enzymatic hydrolysis of alkaline OPF fibre were investigated in an attempt to improve process performance. In this study, OPF biomass was pretreated with 4.42{\%} NaOH at 100 °C for 58.31 min, resulting in significant disruption as characterised by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM). Alkaline pretreatment of OPF biomass improved enzymatic biodegradability, and glucan recovery by the Cellic Ctec2 enzyme was more effective than the conventional Celluclast 1.5 L cellulase enzyme. Synergistic effects of stirring speed, surfactant Triton X-100 loading, and β-glucosidase supplement on enzymatic hydrolysis were assessed using statistical experimental design. Under optimal conditions (450 rpm, 1.31{\%}, and 0.14 pNPGU/FPU), 88{\%} conversion of glucan was obtained from alkaline OPF, which is equivalent to the conversion from commercial cellulose (microcrystalline cellulose, MCC). Enzymatic hydrolysis of pretreated OPF was further improved at high agitation speeds. Synergy between agitation speed and surfactant loading interactions with β-glucosidase supplement enhanced glucose production due to the efficient mixing and availability of cellulose to be adsorbed by cellulase.",
keywords = "Cellulase, Enzyme hydrolysis, Glucose, Oil palm frond, Sodium hydroxide pretreatment",
author = "Zain, {Masniroszaime Md} and Mohammad, {Abdul Wahab} and Shuhaida Harun and Fauzi, {Nurul Aina} and Hairom, {Nur Hanis Hayati}",
year = "2018",
month = "10",
day = "15",
doi = "10.1016/j.indcrop.2018.06.037",
language = "English",
volume = "122",
pages = "617--626",
journal = "Industrial Crops and Products",
issn = "0926-6690",
publisher = "Elsevier",

}

TY - JOUR

T1 - Synergistic effects on process parameters to enhance enzymatic hydrolysis of alkaline oil palm fronds

AU - Zain, Masniroszaime Md

AU - Mohammad, Abdul Wahab

AU - Harun, Shuhaida

AU - Fauzi, Nurul Aina

AU - Hairom, Nur Hanis Hayati

PY - 2018/10/15

Y1 - 2018/10/15

N2 - Due to an increasing demand for more sustainable and renewable resources, there has been strong interest in utilizing biomass as a source for cleaner production of energy and chemicals. In this work, the lignocellulosic elements of oil palm frond (OPF) biomass were assessed as an alternate sugar feedstock for biofuel and bioproducts production. At present, long hydrolysis times and high enzymatic loadings hinder commercialisation and large-scale utilisation of enzymatic hydrolysis of lignocellulosic biomass. Thus, various process parameters of enzymatic hydrolysis of alkaline OPF fibre were investigated in an attempt to improve process performance. In this study, OPF biomass was pretreated with 4.42% NaOH at 100 °C for 58.31 min, resulting in significant disruption as characterised by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM). Alkaline pretreatment of OPF biomass improved enzymatic biodegradability, and glucan recovery by the Cellic Ctec2 enzyme was more effective than the conventional Celluclast 1.5 L cellulase enzyme. Synergistic effects of stirring speed, surfactant Triton X-100 loading, and β-glucosidase supplement on enzymatic hydrolysis were assessed using statistical experimental design. Under optimal conditions (450 rpm, 1.31%, and 0.14 pNPGU/FPU), 88% conversion of glucan was obtained from alkaline OPF, which is equivalent to the conversion from commercial cellulose (microcrystalline cellulose, MCC). Enzymatic hydrolysis of pretreated OPF was further improved at high agitation speeds. Synergy between agitation speed and surfactant loading interactions with β-glucosidase supplement enhanced glucose production due to the efficient mixing and availability of cellulose to be adsorbed by cellulase.

AB - Due to an increasing demand for more sustainable and renewable resources, there has been strong interest in utilizing biomass as a source for cleaner production of energy and chemicals. In this work, the lignocellulosic elements of oil palm frond (OPF) biomass were assessed as an alternate sugar feedstock for biofuel and bioproducts production. At present, long hydrolysis times and high enzymatic loadings hinder commercialisation and large-scale utilisation of enzymatic hydrolysis of lignocellulosic biomass. Thus, various process parameters of enzymatic hydrolysis of alkaline OPF fibre were investigated in an attempt to improve process performance. In this study, OPF biomass was pretreated with 4.42% NaOH at 100 °C for 58.31 min, resulting in significant disruption as characterised by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM). Alkaline pretreatment of OPF biomass improved enzymatic biodegradability, and glucan recovery by the Cellic Ctec2 enzyme was more effective than the conventional Celluclast 1.5 L cellulase enzyme. Synergistic effects of stirring speed, surfactant Triton X-100 loading, and β-glucosidase supplement on enzymatic hydrolysis were assessed using statistical experimental design. Under optimal conditions (450 rpm, 1.31%, and 0.14 pNPGU/FPU), 88% conversion of glucan was obtained from alkaline OPF, which is equivalent to the conversion from commercial cellulose (microcrystalline cellulose, MCC). Enzymatic hydrolysis of pretreated OPF was further improved at high agitation speeds. Synergy between agitation speed and surfactant loading interactions with β-glucosidase supplement enhanced glucose production due to the efficient mixing and availability of cellulose to be adsorbed by cellulase.

KW - Cellulase

KW - Enzyme hydrolysis

KW - Glucose

KW - Oil palm frond

KW - Sodium hydroxide pretreatment

UR - http://www.scopus.com/inward/record.url?scp=85048705521&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85048705521&partnerID=8YFLogxK

U2 - 10.1016/j.indcrop.2018.06.037

DO - 10.1016/j.indcrop.2018.06.037

M3 - Article

AN - SCOPUS:85048705521

VL - 122

SP - 617

EP - 626

JO - Industrial Crops and Products

JF - Industrial Crops and Products

SN - 0926-6690

ER -