Susceptibility testing of clinical mould isolates in Malaysia with the E-test and M38-A methods

Jacinta Santhanam, Ang Kok Teong, Fairuz Amran

Research output: Contribution to journalArticle

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Abstract

Susceptibility testing of filamentous fungal isolates was carried out with the aim of obtaining susceptibility data for Malaysian clinical isolates and to determine the agreement between two methods of susceptibility testing. Fifteen fungal isolates comprising of Aspergillus sp., Fusarium sp. and Cladosporium sp. were evaluated for susceptibility to amphotericin B with E-test and M38-A broth microdilution methods. Susceptibility to itraconazole and voriconazole was also determined with E-test method. With the M38-A method, nine Aspergillus sp. and two Cladosporium sp. isolates were found to be sensitive (MIC 2 μg mL-1), while three Fusarium sp. isolates appeared resistant (MIC≥8 μg mL-1) to Amphotericin B. The E-test showed MIC values which were much lower (0.002-0.5 μg mL-1) for six Aspergillus sp. isolates and the two Cladosporium sp. isolates, while for Fusarium sp., MIC was ≥ μg mL-1. Almost all isolates, other than Fusarium sp., were uniformly more susceptible to voriconazole (MIC: 0.008-0.5 μg mL-1) than itraconazole (MIC: 0.006-6 μg mL-1). In conclusion, Fusarium sp. showed least susceptibility to all antifungals tested. E-test and M38-A methods generated low agreement in determining MIC of amphotericin B and the agreement between the two test results was highest when results were read at 72 h incubation.

Original languageEnglish
Pages (from-to)94-97
Number of pages4
JournalJournal of Medical Sciences
Volume8
Issue number1
Publication statusPublished - 1 Jan 2008

Fingerprint

Malaysia
Fusarium
Fungi
Cladosporium
Amphotericin B
Aspergillus
Itraconazole
Voriconazole

Keywords

  • Agreement
  • E-test
  • Fungi
  • M38-A
  • Malaysia
  • Susceptibility

ASJC Scopus subject areas

  • Physiology
  • Medicine(all)

Cite this

Susceptibility testing of clinical mould isolates in Malaysia with the E-test and M38-A methods. / Santhanam, Jacinta; Teong, Ang Kok; Amran, Fairuz.

In: Journal of Medical Sciences, Vol. 8, No. 1, 01.01.2008, p. 94-97.

Research output: Contribution to journalArticle

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