Surface modification of electrospun poly(methyl methacrylate) (PMMA) nanofibers for the development of in vitro respiratory epithelium model

A. R. Rabiatul, Lokanathan Yogeswaran, C. M. Rohaina, Shiplu Roy Chowdhury, B. S. Aminuddin, Ruszymah Idrus

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11 Citations (Scopus)

Abstract

Scaffold design is an important aspect of in vitro model development. In this study, nanoscaffold surface modification, namely UV radiation and genipin cross-linking to immobilize collagen on the surface of electrospun poly (methyl methacrylate) (PMMA) nanofiber sheet was investigated. Samples were divided into four groups; PMMA nanofibers (PMMA), collagen-coated PMMA nanofibers (PMMACOL), genipin cross-linked collagen-coated PMMA nanofibers (PMMAGEN), and UV-irradiated collagen-coated PMMA nanofibers (PMMAUV). 6 h of UV radiation significantly reduced the hydrophobicity of PMMA nanofibers from (131.88° ± 1.33°) to (110.04° ± 0.27°) (p <0.05). The amount of collagen immobilized was significantly higher in PMMAGEN group (239.36 ± 16.63 g collagen/mg nanofibers) (p <0.05) compared to the other groups. RECs on all scaffold expressed epithelial cell-specific markers (CK18 and CK14), mucin-producing cell marker (MUC5Ac) and were actively proliferating, based on the positive expression of Ki67. Total number of attached cells was significantly the highest in PMMAUV group on day 9 (6.44 × 104 ± 2.77 × 104 cells/cm2) and it has the highest proliferation rate from day 4 to 9 (0.005 ± 0.003 h-1) compared to the other groups. Even though PMMAGEN group showed the highest collagen adsorption, in terms of cells attachment and proliferation, PMMAUV group showed a better outcome compared to the other groups. Thus, PMMAUV scaffold is more suitable to be used in the construction of in vitro respiratory epithelial model.

Original languageEnglish
Pages (from-to)1297-1311
Number of pages15
JournalJournal of Biomaterials Science, Polymer Edition
Volume26
Issue number17
DOIs
Publication statusPublished - 22 Nov 2015

Fingerprint

Nanofibers
Respiratory Mucosa
Polymethyl Methacrylate
Polymethyl methacrylates
Collagen
Surface treatment
Scaffolds
Ultraviolet radiation
Radiation
Mucins
Hydrophobicity
In Vitro Techniques
Hydrophobic and Hydrophilic Interactions
Adsorption
Cell Count
Epithelial Cells
Cell Proliferation

Keywords

  • airway epithelium
  • collagen type 1
  • genipin cross-linking
  • tissue engineering
  • UV radiation

ASJC Scopus subject areas

  • Biophysics
  • Biomaterials
  • Bioengineering
  • Biomedical Engineering

Cite this

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title = "Surface modification of electrospun poly(methyl methacrylate) (PMMA) nanofibers for the development of in vitro respiratory epithelium model",
abstract = "Scaffold design is an important aspect of in vitro model development. In this study, nanoscaffold surface modification, namely UV radiation and genipin cross-linking to immobilize collagen on the surface of electrospun poly (methyl methacrylate) (PMMA) nanofiber sheet was investigated. Samples were divided into four groups; PMMA nanofibers (PMMA), collagen-coated PMMA nanofibers (PMMACOL), genipin cross-linked collagen-coated PMMA nanofibers (PMMAGEN), and UV-irradiated collagen-coated PMMA nanofibers (PMMAUV). 6 h of UV radiation significantly reduced the hydrophobicity of PMMA nanofibers from (131.88° ± 1.33°) to (110.04° ± 0.27°) (p <0.05). The amount of collagen immobilized was significantly higher in PMMAGEN group (239.36 ± 16.63 g collagen/mg nanofibers) (p <0.05) compared to the other groups. RECs on all scaffold expressed epithelial cell-specific markers (CK18 and CK14), mucin-producing cell marker (MUC5Ac) and were actively proliferating, based on the positive expression of Ki67. Total number of attached cells was significantly the highest in PMMAUV group on day 9 (6.44 × 104 ± 2.77 × 104 cells/cm2) and it has the highest proliferation rate from day 4 to 9 (0.005 ± 0.003 h-1) compared to the other groups. Even though PMMAGEN group showed the highest collagen adsorption, in terms of cells attachment and proliferation, PMMAUV group showed a better outcome compared to the other groups. Thus, PMMAUV scaffold is more suitable to be used in the construction of in vitro respiratory epithelial model.",
keywords = "airway epithelium, collagen type 1, genipin cross-linking, tissue engineering, UV radiation",
author = "Rabiatul, {A. R.} and Lokanathan Yogeswaran and Rohaina, {C. M.} and Chowdhury, {Shiplu Roy} and Aminuddin, {B. S.} and Ruszymah Idrus",
year = "2015",
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T1 - Surface modification of electrospun poly(methyl methacrylate) (PMMA) nanofibers for the development of in vitro respiratory epithelium model

AU - Rabiatul, A. R.

AU - Yogeswaran, Lokanathan

AU - Rohaina, C. M.

AU - Chowdhury, Shiplu Roy

AU - Aminuddin, B. S.

AU - Idrus, Ruszymah

PY - 2015/11/22

Y1 - 2015/11/22

N2 - Scaffold design is an important aspect of in vitro model development. In this study, nanoscaffold surface modification, namely UV radiation and genipin cross-linking to immobilize collagen on the surface of electrospun poly (methyl methacrylate) (PMMA) nanofiber sheet was investigated. Samples were divided into four groups; PMMA nanofibers (PMMA), collagen-coated PMMA nanofibers (PMMACOL), genipin cross-linked collagen-coated PMMA nanofibers (PMMAGEN), and UV-irradiated collagen-coated PMMA nanofibers (PMMAUV). 6 h of UV radiation significantly reduced the hydrophobicity of PMMA nanofibers from (131.88° ± 1.33°) to (110.04° ± 0.27°) (p <0.05). The amount of collagen immobilized was significantly higher in PMMAGEN group (239.36 ± 16.63 g collagen/mg nanofibers) (p <0.05) compared to the other groups. RECs on all scaffold expressed epithelial cell-specific markers (CK18 and CK14), mucin-producing cell marker (MUC5Ac) and were actively proliferating, based on the positive expression of Ki67. Total number of attached cells was significantly the highest in PMMAUV group on day 9 (6.44 × 104 ± 2.77 × 104 cells/cm2) and it has the highest proliferation rate from day 4 to 9 (0.005 ± 0.003 h-1) compared to the other groups. Even though PMMAGEN group showed the highest collagen adsorption, in terms of cells attachment and proliferation, PMMAUV group showed a better outcome compared to the other groups. Thus, PMMAUV scaffold is more suitable to be used in the construction of in vitro respiratory epithelial model.

AB - Scaffold design is an important aspect of in vitro model development. In this study, nanoscaffold surface modification, namely UV radiation and genipin cross-linking to immobilize collagen on the surface of electrospun poly (methyl methacrylate) (PMMA) nanofiber sheet was investigated. Samples were divided into four groups; PMMA nanofibers (PMMA), collagen-coated PMMA nanofibers (PMMACOL), genipin cross-linked collagen-coated PMMA nanofibers (PMMAGEN), and UV-irradiated collagen-coated PMMA nanofibers (PMMAUV). 6 h of UV radiation significantly reduced the hydrophobicity of PMMA nanofibers from (131.88° ± 1.33°) to (110.04° ± 0.27°) (p <0.05). The amount of collagen immobilized was significantly higher in PMMAGEN group (239.36 ± 16.63 g collagen/mg nanofibers) (p <0.05) compared to the other groups. RECs on all scaffold expressed epithelial cell-specific markers (CK18 and CK14), mucin-producing cell marker (MUC5Ac) and were actively proliferating, based on the positive expression of Ki67. Total number of attached cells was significantly the highest in PMMAUV group on day 9 (6.44 × 104 ± 2.77 × 104 cells/cm2) and it has the highest proliferation rate from day 4 to 9 (0.005 ± 0.003 h-1) compared to the other groups. Even though PMMAGEN group showed the highest collagen adsorption, in terms of cells attachment and proliferation, PMMAUV group showed a better outcome compared to the other groups. Thus, PMMAUV scaffold is more suitable to be used in the construction of in vitro respiratory epithelial model.

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