Surface modification of cellulose nanomaterial for urea biosensor application

Wan Elina Faradilla Wan Khalid, Yook Heng Lee, Mohamad Nasir Mat Arip

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Cellulose nanomaterial with rod-like structure and highly crystalline order, usually formed by elimination of the amorphous region from cellulose during acid hydrolysis. Cellulose nanomaterial with the property of biocompatibility and nontoxicity can be used for enzyme immobilization. In this work, urease enzyme was used as a model enzyme to study the surface modification of cellulose nanomaterial and its potential for biosensor application. The cellulose nanocrystal (CNC) surface was modified using 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation to introduce the carboxyl group at C6 primary alcohol. The success of enzyme immobilization and surface modification was confirmed using chemical tests and measured using UV-Visible spectrophotometer. The immobilization strategy was then applied for biosensor application for urea detection. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques were used for electroanalytical characterization of the urea biosensor.

Original languageEnglish
Pages (from-to)941-949
Number of pages9
JournalSains Malaysiana
Volume47
Issue number5
DOIs
Publication statusPublished - 1 May 2018

Fingerprint

nanomaterials
biosensors
cellulose
urea
immobilized enzymes
nanocrystals
biocompatibility
spectrophotometers
acid hydrolysis
urease
enzymes
alcohols
oxidation
testing

Keywords

  • Biosensor
  • Cellulose nanomaterial
  • Enzyme immobilization
  • Surface modification

ASJC Scopus subject areas

  • General

Cite this

Surface modification of cellulose nanomaterial for urea biosensor application. / Khalid, Wan Elina Faradilla Wan; Lee, Yook Heng; Arip, Mohamad Nasir Mat.

In: Sains Malaysiana, Vol. 47, No. 5, 01.05.2018, p. 941-949.

Research output: Contribution to journalArticle

Khalid, Wan Elina Faradilla Wan ; Lee, Yook Heng ; Arip, Mohamad Nasir Mat. / Surface modification of cellulose nanomaterial for urea biosensor application. In: Sains Malaysiana. 2018 ; Vol. 47, No. 5. pp. 941-949.
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