Surface functionalization of highly luminescent carbon nanodots from Dioscorea hispida with polyethylene glycol and branched polyethyleneimine and their in vitro study

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Abstract

Synthesized carbon dots (C-dots) from Dioscorea hispida (Gadong tuber) starch were modified with passivating agents such as O,O-bis(3-aminopropyl)polyethylene glycol 1500 (PEG1500N) and branched polyethyleneimine (BPEI) to increase the functional efficiency of C-dots to be used in biological investigations. After surface modification, the C-dots decreased in size from 6–25 to 3–20 nm without changing their morphology. The modified C-dots were fluorescent, and the fluorescence peak gradually shifted to a longer excitation wavelength (from 420 to 500). Upon modification, thereby elucidating a competitive quantum yield of 15% (C-dots-PEG1500N) and 12.6% (C-dots-BPEI). The UV-visible spectrum of the C-dots modified with PEG1500N contained an absorption peak at 290 nm, whereas, that of the C-dots modified with BPEI contained peak at 360. Fourier-transform infrared analysis showed a peak at 1700 cm−1 (C-dots-PEG1500N) and 1697 cm−1 (C-dots-BPEI) that corresponds to the amide (–HCONH–) carbonyl bond, indicating that PEG1500N and BPEI had been successfully passivated on the surface of C-dots. To examine photothermal response, irradiation was carried out for 5 min using three different instruments which is UV-lamp (365 nm), visible lamp and laser (532 nm, 1 W/cm2) where temperature for each sample increased respectively. C-dots demonstrated not losing their luminescent properties upon irradiation to UV-lamp and laser for 30 min. Contrary, the intensity of fluorescence reduced when it exposed to the visible lamp for the same period. In vitro studies of the modified C-dots with zebrafish (Danio rerio) revealed that C-dots-PEG1500N is non-toxic while C-dots-BPEI is highly toxic to the fish embryos. Significantly, this study has successfully demonstrated that Gadong tuber starch can be used as a starch-based modifier for C-dots; which possibly can be utilized as a nanocarrier with thermal sensing properties.

Original languageEnglish
JournalJournal of King Saud University - Science
DOIs
Publication statusAccepted/In press - 1 Jan 2018

Fingerprint

Polyethyleneimine
Carbon
Starch
Ultraviolet lamps
Electric lamps
Fluorescence
Irradiation
Lasers
Poisons
Quantum yield

Keywords

  • BPEI
  • C-dots
  • Gadong starch
  • Luminescence
  • Nanoparticles
  • PEG

ASJC Scopus subject areas

  • General

Cite this

@article{1c9f5521c2544a9684de49a6daca220e,
title = "Surface functionalization of highly luminescent carbon nanodots from Dioscorea hispida with polyethylene glycol and branched polyethyleneimine and their in vitro study",
abstract = "Synthesized carbon dots (C-dots) from Dioscorea hispida (Gadong tuber) starch were modified with passivating agents such as O,O-bis(3-aminopropyl)polyethylene glycol 1500 (PEG1500N) and branched polyethyleneimine (BPEI) to increase the functional efficiency of C-dots to be used in biological investigations. After surface modification, the C-dots decreased in size from 6–25 to 3–20 nm without changing their morphology. The modified C-dots were fluorescent, and the fluorescence peak gradually shifted to a longer excitation wavelength (from 420 to 500). Upon modification, thereby elucidating a competitive quantum yield of 15{\%} (C-dots-PEG1500N) and 12.6{\%} (C-dots-BPEI). The UV-visible spectrum of the C-dots modified with PEG1500N contained an absorption peak at 290 nm, whereas, that of the C-dots modified with BPEI contained peak at 360. Fourier-transform infrared analysis showed a peak at 1700 cm−1 (C-dots-PEG1500N) and 1697 cm−1 (C-dots-BPEI) that corresponds to the amide (–HCONH–) carbonyl bond, indicating that PEG1500N and BPEI had been successfully passivated on the surface of C-dots. To examine photothermal response, irradiation was carried out for 5 min using three different instruments which is UV-lamp (365 nm), visible lamp and laser (532 nm, 1 W/cm2) where temperature for each sample increased respectively. C-dots demonstrated not losing their luminescent properties upon irradiation to UV-lamp and laser for 30 min. Contrary, the intensity of fluorescence reduced when it exposed to the visible lamp for the same period. In vitro studies of the modified C-dots with zebrafish (Danio rerio) revealed that C-dots-PEG1500N is non-toxic while C-dots-BPEI is highly toxic to the fish embryos. Significantly, this study has successfully demonstrated that Gadong tuber starch can be used as a starch-based modifier for C-dots; which possibly can be utilized as a nanocarrier with thermal sensing properties.",
keywords = "BPEI, C-dots, Gadong starch, Luminescence, Nanoparticles, PEG",
author = "Sonthanasamy, {Regina Sisika A.} and Sa`Ariwijaya, {Mohd Shazrul Fazry} and {Mohd. Yamin}, Bohari and {Mat Lazim}, {Mohamad Azwani Shah}",
year = "2018",
month = "1",
day = "1",
doi = "10.1016/j.jksus.2018.05.004",
language = "English",
journal = "Journal of King Saud University - Science",
issn = "1018-3647",
publisher = "King Saud University",

}

TY - JOUR

T1 - Surface functionalization of highly luminescent carbon nanodots from Dioscorea hispida with polyethylene glycol and branched polyethyleneimine and their in vitro study

AU - Sonthanasamy, Regina Sisika A.

AU - Sa`Ariwijaya, Mohd Shazrul Fazry

AU - Mohd. Yamin, Bohari

AU - Mat Lazim, Mohamad Azwani Shah

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Synthesized carbon dots (C-dots) from Dioscorea hispida (Gadong tuber) starch were modified with passivating agents such as O,O-bis(3-aminopropyl)polyethylene glycol 1500 (PEG1500N) and branched polyethyleneimine (BPEI) to increase the functional efficiency of C-dots to be used in biological investigations. After surface modification, the C-dots decreased in size from 6–25 to 3–20 nm without changing their morphology. The modified C-dots were fluorescent, and the fluorescence peak gradually shifted to a longer excitation wavelength (from 420 to 500). Upon modification, thereby elucidating a competitive quantum yield of 15% (C-dots-PEG1500N) and 12.6% (C-dots-BPEI). The UV-visible spectrum of the C-dots modified with PEG1500N contained an absorption peak at 290 nm, whereas, that of the C-dots modified with BPEI contained peak at 360. Fourier-transform infrared analysis showed a peak at 1700 cm−1 (C-dots-PEG1500N) and 1697 cm−1 (C-dots-BPEI) that corresponds to the amide (–HCONH–) carbonyl bond, indicating that PEG1500N and BPEI had been successfully passivated on the surface of C-dots. To examine photothermal response, irradiation was carried out for 5 min using three different instruments which is UV-lamp (365 nm), visible lamp and laser (532 nm, 1 W/cm2) where temperature for each sample increased respectively. C-dots demonstrated not losing their luminescent properties upon irradiation to UV-lamp and laser for 30 min. Contrary, the intensity of fluorescence reduced when it exposed to the visible lamp for the same period. In vitro studies of the modified C-dots with zebrafish (Danio rerio) revealed that C-dots-PEG1500N is non-toxic while C-dots-BPEI is highly toxic to the fish embryos. Significantly, this study has successfully demonstrated that Gadong tuber starch can be used as a starch-based modifier for C-dots; which possibly can be utilized as a nanocarrier with thermal sensing properties.

AB - Synthesized carbon dots (C-dots) from Dioscorea hispida (Gadong tuber) starch were modified with passivating agents such as O,O-bis(3-aminopropyl)polyethylene glycol 1500 (PEG1500N) and branched polyethyleneimine (BPEI) to increase the functional efficiency of C-dots to be used in biological investigations. After surface modification, the C-dots decreased in size from 6–25 to 3–20 nm without changing their morphology. The modified C-dots were fluorescent, and the fluorescence peak gradually shifted to a longer excitation wavelength (from 420 to 500). Upon modification, thereby elucidating a competitive quantum yield of 15% (C-dots-PEG1500N) and 12.6% (C-dots-BPEI). The UV-visible spectrum of the C-dots modified with PEG1500N contained an absorption peak at 290 nm, whereas, that of the C-dots modified with BPEI contained peak at 360. Fourier-transform infrared analysis showed a peak at 1700 cm−1 (C-dots-PEG1500N) and 1697 cm−1 (C-dots-BPEI) that corresponds to the amide (–HCONH–) carbonyl bond, indicating that PEG1500N and BPEI had been successfully passivated on the surface of C-dots. To examine photothermal response, irradiation was carried out for 5 min using three different instruments which is UV-lamp (365 nm), visible lamp and laser (532 nm, 1 W/cm2) where temperature for each sample increased respectively. C-dots demonstrated not losing their luminescent properties upon irradiation to UV-lamp and laser for 30 min. Contrary, the intensity of fluorescence reduced when it exposed to the visible lamp for the same period. In vitro studies of the modified C-dots with zebrafish (Danio rerio) revealed that C-dots-PEG1500N is non-toxic while C-dots-BPEI is highly toxic to the fish embryos. Significantly, this study has successfully demonstrated that Gadong tuber starch can be used as a starch-based modifier for C-dots; which possibly can be utilized as a nanocarrier with thermal sensing properties.

KW - BPEI

KW - C-dots

KW - Gadong starch

KW - Luminescence

KW - Nanoparticles

KW - PEG

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U2 - 10.1016/j.jksus.2018.05.004

DO - 10.1016/j.jksus.2018.05.004

M3 - Article

JO - Journal of King Saud University - Science

JF - Journal of King Saud University - Science

SN - 1018-3647

ER -