Surface Antigen Profiling of Helicobacter pylori-Infected and -Uninfected Gastric Cancer Cells Using Antibody Microarray

Asif Sukri, Alfizah Hanafiah, Nik Ritza Kosai Nik Mahmood, Mustafa Mohamed Taher, Isa Mohamed Rose

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Comprehensive immunophenotyping cluster of differentiation (CD) antigens in gastric adenocarcinoma, specifically between Helicobacter pylori-infected and -uninfected gastric cancer patients by using DotScan antibody microarray has not been conducted. Current immunophenotyping techniques include flow cytometry and immunohistochemistry are limited to the use of few antibodies for parallel examination. We used DotScan antibody microarray consisting 144 CD antibodies to determine the distribution of CD antigens in gastric adenocarcinoma cells and to elucidate the effect of H. pylori infection toward CD antigen expression in gastric cancer. Methods: Mixed leukocytes population derived from gastric adenocarcinoma patients were immunophenotyped using DotScan antibody microarray. AGS cells were infected with H. pylori strains and cells were captured on DotScan slides. Results: Cluster of differentiation antigens involved in perpetuating the tolerance of immune cells to tumor cells was upregulated in gastric adenocarcinoma cells compared to normal cells. CD279 which is essential in T cells apoptosis was found to be upregulated in normal cells. Remarkably, H. pylori-infected gastric cancer patients exhibited upregulated expression of CD27 that important in maintenance of T cells. Infection of cagA+ H. pylori with AGS cells increased CD antigens expression which involved in cancer stem cell while cagA− H. pylori polarized AGS cells to express immune-regulatory CD antigens. Increased CD antigens expression in AGS cells infected with cagA+ H. pylori were also detected in H. pylori-infected gastric cancer patients. Conclusion: This study suggests the tolerance of immune system toward tumor cells in gastric cancer and distinct mechanisms of immune responses exploited by different H. pylori strains.

Original languageEnglish
Pages (from-to)417-427
Number of pages11
JournalHelicobacter
Volume21
Issue number5
DOIs
Publication statusPublished - 1 Oct 2016

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Surface Antigens
Helicobacter pylori
Stomach Neoplasms
CD Antigens
Antibodies
Stomach
Adenocarcinoma
Immunophenotyping
T-Lymphocytes
Immune Tolerance
Neoplastic Stem Cells
Helicobacter Infections
Immune System
Neoplasms
Flow Cytometry
Leukocytes
Immunohistochemistry
Maintenance
Apoptosis

Keywords

  • Antibody microarray
  • cagA-positive
  • CD antigens
  • Gastric cancer
  • Helicobacter pylori

ASJC Scopus subject areas

  • Gastroenterology
  • Infectious Diseases

Cite this

Surface Antigen Profiling of Helicobacter pylori-Infected and -Uninfected Gastric Cancer Cells Using Antibody Microarray. / Sukri, Asif; Hanafiah, Alfizah; Nik Mahmood, Nik Ritza Kosai; Mohamed Taher, Mustafa; Mohamed Rose, Isa.

In: Helicobacter, Vol. 21, No. 5, 01.10.2016, p. 417-427.

Research output: Contribution to journalArticle

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