Superolein based intravenous lipid emulsion 20% W/W physicochemical characterization, stability and its effect on liver status

Mohd Haz Hairul Amran, Mohd Fairuz Danik, Mohd Shakrie Palan Abdullah, Mohd Hanif Zulfakar, Ahmad Fuad Shamsuddin

Research output: Contribution to journalArticle

Abstract

Long chain triglycerides (LCT) from soya oil have been reported to cause hepatic abnormalities among patients receiving parenteral nutrition. A new source for LCT is needed to support current therapy. The aims of this study were to develop a good and stable superolein oil based intravenous lipid emulsion 20% (SoLE), and to determine its effect on liver status of an animal model. The emulsion was prepared using superolein oil and medium chain triglyceride oil (1:1), 1.2 g lecithin and homogenized by high pressure homogenization method. The physical stability study was performed based on real-time, accelerated, force degradation and environmental degradation. Chemical stability was measured based on fatty acids and vitamin E contents, peroxide value and acidity value. As the animal model, three rabbits were administered with 1.5 g/kg SoLE for 5 h and repeated daily for three days. The SoLE was found to have good physicochemical characters: 229.07±1.57 nm of particle size; 0.00% of particle > 5 µm; 342.00±1.00 mOsm/kg of osmolarity; -46.67±4.40 mV of zeta potential; 1.72±0.04 cP of viscosity; 7.83±0.06 of pH; 0.80±0.06 kcal/g calorie; 1.74±0.05 mEqO2/kg of peroxide value; and 0.03±0.01% of acidity value, and excellent physicochemical stabilities for up to six months of storage at various temperatures and conditions. The level of plasma lipid and plasma liver enzymes profiles of the rabbits remained statistically unchanged. This study has successfully developed a stable SoLE 20% and it was found to be safe to the liver of the animal model.

Original languageEnglish
Pages (from-to)1043-1054
Number of pages12
JournalSains Malaysiana
Volume48
Issue number5
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

emulsions
oils
liver
lipids
long chain triacylglycerols
animal models
peroxide value
acidity
rabbits
medium chain triacylglycerols
osmolarity
environmental degradation
parenteral feeding
homogenization
phosphatidylcholines
blood lipids
vitamin E
particle size
viscosity
fatty acids

Keywords

  • Animal model
  • Intravenous lipid emulsion
  • Physicochemical
  • Stability
  • Superolein oil

ASJC Scopus subject areas

  • General

Cite this

Superolein based intravenous lipid emulsion 20% W/W physicochemical characterization, stability and its effect on liver status. / Amran, Mohd Haz Hairul; Danik, Mohd Fairuz; Abdullah, Mohd Shakrie Palan; Zulfakar, Mohd Hanif; Shamsuddin, Ahmad Fuad.

In: Sains Malaysiana, Vol. 48, No. 5, 01.01.2019, p. 1043-1054.

Research output: Contribution to journalArticle

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abstract = "Long chain triglycerides (LCT) from soya oil have been reported to cause hepatic abnormalities among patients receiving parenteral nutrition. A new source for LCT is needed to support current therapy. The aims of this study were to develop a good and stable superolein oil based intravenous lipid emulsion 20{\%} (SoLE), and to determine its effect on liver status of an animal model. The emulsion was prepared using superolein oil and medium chain triglyceride oil (1:1), 1.2 g lecithin and homogenized by high pressure homogenization method. The physical stability study was performed based on real-time, accelerated, force degradation and environmental degradation. Chemical stability was measured based on fatty acids and vitamin E contents, peroxide value and acidity value. As the animal model, three rabbits were administered with 1.5 g/kg SoLE for 5 h and repeated daily for three days. The SoLE was found to have good physicochemical characters: 229.07±1.57 nm of particle size; 0.00{\%} of particle > 5 µm; 342.00±1.00 mOsm/kg of osmolarity; -46.67±4.40 mV of zeta potential; 1.72±0.04 cP of viscosity; 7.83±0.06 of pH; 0.80±0.06 kcal/g calorie; 1.74±0.05 mEqO2/kg of peroxide value; and 0.03±0.01{\%} of acidity value, and excellent physicochemical stabilities for up to six months of storage at various temperatures and conditions. The level of plasma lipid and plasma liver enzymes profiles of the rabbits remained statistically unchanged. This study has successfully developed a stable SoLE 20{\%} and it was found to be safe to the liver of the animal model.",
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