Sulfate-reducing bacteria metabolite detection using GC-MS

M. F. Nawawi, Fathul Karim Sahrani, Kamalrul Azlan Azizan, M. M. Ali, Asmat Ahmad, Gires Usup

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This research was conducted to investigate and compare the various metabolite, produced from the metabolism of two different SRB strain, that were involved in the biocorrosion process. Stainless steel coupons were immersed in two strains of sulfatereducing bacteria, A1H1 and EB3 (designated as SRB1 and SRB2 repectively) were isolated from Port Dickson marine vicinity that were grown in VMNI medium. The immersion period were set for eight days. Analysis of Gas Chromatography- Mass Spectrometry (GC-MS) was conducted using -trimethylsilyl (TMS) of N-methyl-N-trimethylsilylfluoracetamide (MSTFA). The data were than analyzed using Partial Least Squares Discriminant Analysis (PLS-DA) method to discriminate the unique metabolite according to each strain. The result showed that SRB1 generates less metabolite but high in concentration. Meanwhile, SRB2 shows a variety production of metabolites but less in concentration. Both strains share the same metabolism in the production of nitrogen based substance and production of norvaline and pentanoic acid. SRB1 shows a very distinct feature as the production of ribitol was spotted in its metabolism where it is usually associated with growth. SRB2 showed a very close usage of sulphur by production of methionine. These results suggest that different SRB strain produced different number and type of metabolites in the biocorrosion process.

Original languageEnglish
Pages (from-to)41-51
Number of pages11
JournalMalaysian Applied Biology
Volume43
Issue number1
Publication statusPublished - 2014

Fingerprint

sulfate-reducing bacteria
Gas Chromatography-Mass Spectrometry
Sulfates
metabolites
Bacteria
Pentanoic Acids
Ribitol
Stainless Steel
metabolism
Discriminant Analysis
Immersion
Least-Squares Analysis
Sulfur
Methionine
Nitrogen
stainless steel
discriminant analysis
least squares
methionine
Growth

Keywords

  • Atomic Force Microscopy
  • Biofilm
  • Extra Polymeric Substances
  • Sulphate-reducing bacteria

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Sulfate-reducing bacteria metabolite detection using GC-MS. / Nawawi, M. F.; Sahrani, Fathul Karim; Azizan, Kamalrul Azlan; Ali, M. M.; Ahmad, Asmat; Usup, Gires.

In: Malaysian Applied Biology, Vol. 43, No. 1, 2014, p. 41-51.

Research output: Contribution to journalArticle

Nawawi, MF, Sahrani, FK, Azizan, KA, Ali, MM, Ahmad, A & Usup, G 2014, 'Sulfate-reducing bacteria metabolite detection using GC-MS', Malaysian Applied Biology, vol. 43, no. 1, pp. 41-51.
Nawawi, M. F. ; Sahrani, Fathul Karim ; Azizan, Kamalrul Azlan ; Ali, M. M. ; Ahmad, Asmat ; Usup, Gires. / Sulfate-reducing bacteria metabolite detection using GC-MS. In: Malaysian Applied Biology. 2014 ; Vol. 43, No. 1. pp. 41-51.
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