Subspecies identification of captive Orang Utan in Melaka based on D-loop mitochondria DNA

Siti Norsyuhada Kamaluddin, Salmah Yaakop, Wan Mohd Razi Idris, Jeffrine Japning Rovie-Ryan, Badrul Munir Md. Zain

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Mitochondrial DNA of Bornean Orang Utan populations suggests that there are three different subspecies (Pongo pygmaeus pygmaeus; Sarawak & Northwest Kalimantan, P. p. wurmbii; Southern West Kalimantan and Central Kalimantan, P. p. morio; East Kalimantan and Sabah). The subspecies of Orang Utans in captivity are difficult to determine through morphological observation. Thus, misidentification by ranger or zoo staffs leads to unwanted consequences especially towards conservation efforts of Orang Utan. The main objective of this study was to identify the subspecies and the geographic origin of 10 Orang Utans in Zoo Melaka and A' Famosa by using partial mitochondrial D-loop gene sequences. DNA of all individuals was extracted from FTA Card. Data analyses were performed using Maximum Parsimony, MP and Neighbor Joining, NJ. Molecular phylogeny analysis revealed that all the samples likely belong to one species of Sumatran Orang Utan (P. abelii) and three different subspecies of Bornean Orang Utans (P. p. pygmaeus, P. p. morio, and P. p. wurmbii). The results obtained in this study indirectly help the management of zoos in term of conservation and visitor's education.

Original languageEnglish
Title of host publication2017 UKM FST Postgraduate Colloquium
Subtitle of host publicationProceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1940
ISBN (Electronic)9780735416321
DOIs
Publication statusPublished - 4 Apr 2018
Event2017 UKM FST Postgraduate Colloquium - Selangor, Malaysia
Duration: 12 Jul 201713 Jul 2017

Other

Other2017 UKM FST Postgraduate Colloquium
CountryMalaysia
CitySelangor
Period12/7/1713/7/17

Fingerprint

mitochondria
conservation
deoxyribonucleic acid
cards
genes
education

Keywords

  • mitochondrial
  • Orang Utan
  • phylogeny

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Kamaluddin, S. N., Yaakop, S., Idris, W. M. R., Rovie-Ryan, J. J., & Md. Zain, B. M. (2018). Subspecies identification of captive Orang Utan in Melaka based on D-loop mitochondria DNA. In 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium (Vol. 1940). [020055] American Institute of Physics Inc.. https://doi.org/10.1063/1.5027970

Subspecies identification of captive Orang Utan in Melaka based on D-loop mitochondria DNA. / Kamaluddin, Siti Norsyuhada; Yaakop, Salmah; Idris, Wan Mohd Razi; Rovie-Ryan, Jeffrine Japning; Md. Zain, Badrul Munir.

2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940 American Institute of Physics Inc., 2018. 020055.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kamaluddin, SN, Yaakop, S, Idris, WMR, Rovie-Ryan, JJ & Md. Zain, BM 2018, Subspecies identification of captive Orang Utan in Melaka based on D-loop mitochondria DNA. in 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. vol. 1940, 020055, American Institute of Physics Inc., 2017 UKM FST Postgraduate Colloquium, Selangor, Malaysia, 12/7/17. https://doi.org/10.1063/1.5027970
Kamaluddin SN, Yaakop S, Idris WMR, Rovie-Ryan JJ, Md. Zain BM. Subspecies identification of captive Orang Utan in Melaka based on D-loop mitochondria DNA. In 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940. American Institute of Physics Inc. 2018. 020055 https://doi.org/10.1063/1.5027970
Kamaluddin, Siti Norsyuhada ; Yaakop, Salmah ; Idris, Wan Mohd Razi ; Rovie-Ryan, Jeffrine Japning ; Md. Zain, Badrul Munir. / Subspecies identification of captive Orang Utan in Melaka based on D-loop mitochondria DNA. 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940 American Institute of Physics Inc., 2018.
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