Styryl-lactone goniothalamin inhibits TNF-α-induced NF-κB activation

Barbora Orlikova, Marc Schumacher, Tom Juncker, Choo Chee Yan, Salmaan H. Inayat-Hussain, Shéhérazade Hajjouli, Claudia Cerella, Mario Dicato, Marc Diederich

    Research output: Contribution to journalArticle

    25 Citations (Scopus)

    Abstract

    (R)-(+)-Goniothalamin (GTN), a styryl-lactone isolated from the medicinal plant Goniothalamus macrophyllus, exhibits pharmacological activities including cytotoxic and anti-inflammatory effects. In this study, GTN modulated TNF-α induced NF-κB activation. GTN concentrations up to 20. μM showed low cytotoxic effects in K562 chronic myelogenous leukemia and in Jurkat T cells. Importantly, at these concentrations, no cytotoxicity was observed in healthy peripheral blood mononuclear cells. Our results confirmed that GTN inhibited tumor necrosis factor-α (TNF-α)-induced NF-κB activation in Jurkat and K562 leukemia cells at concentrations as low as 5. μM as shown by reporter gene assays and western blots. Moreover, GTN down-regulated translocation of the p50/p65 heterodimer to the nucleus, prevented binding of NF-κB to its DNA response element and reduced TNF-α-activated interleukin-8 (IL-8) expression. In conclusion, GTN inhibits TNF-α-induced NF-κB activation at non-apoptogenic concentrations in different leukemia cell models without presenting toxicity towards healthy blood cells underlining the anti-leukemic potential of this natural compound.

    Original languageEnglish
    Pages (from-to)572-578
    Number of pages7
    JournalFood and Chemical Toxicology
    Volume59
    DOIs
    Publication statusPublished - Sep 2013

    Fingerprint

    tumor necrosis factors
    Lactones
    lactones
    Tumor Necrosis Factor-alpha
    Chemical activation
    leukemia
    cytotoxicity
    Goniothalamus
    response elements
    Blood Cells
    interleukin-8
    Leukemia
    Blood
    mononuclear leukocytes
    blood cells
    anti-inflammatory activity
    reporter genes
    medicinal plants
    Western blotting
    Jurkat Cells

    Keywords

    • Anti-cancer drug discovery
    • Cancer
    • Inflammation
    • NF-κB
    • Plant natural product

    ASJC Scopus subject areas

    • Food Science
    • Toxicology

    Cite this

    Orlikova, B., Schumacher, M., Juncker, T., Yan, C. C., Inayat-Hussain, S. H., Hajjouli, S., ... Diederich, M. (2013). Styryl-lactone goniothalamin inhibits TNF-α-induced NF-κB activation. Food and Chemical Toxicology, 59, 572-578. https://doi.org/10.1016/j.fct.2013.06.051

    Styryl-lactone goniothalamin inhibits TNF-α-induced NF-κB activation. / Orlikova, Barbora; Schumacher, Marc; Juncker, Tom; Yan, Choo Chee; Inayat-Hussain, Salmaan H.; Hajjouli, Shéhérazade; Cerella, Claudia; Dicato, Mario; Diederich, Marc.

    In: Food and Chemical Toxicology, Vol. 59, 09.2013, p. 572-578.

    Research output: Contribution to journalArticle

    Orlikova, B, Schumacher, M, Juncker, T, Yan, CC, Inayat-Hussain, SH, Hajjouli, S, Cerella, C, Dicato, M & Diederich, M 2013, 'Styryl-lactone goniothalamin inhibits TNF-α-induced NF-κB activation', Food and Chemical Toxicology, vol. 59, pp. 572-578. https://doi.org/10.1016/j.fct.2013.06.051
    Orlikova B, Schumacher M, Juncker T, Yan CC, Inayat-Hussain SH, Hajjouli S et al. Styryl-lactone goniothalamin inhibits TNF-α-induced NF-κB activation. Food and Chemical Toxicology. 2013 Sep;59:572-578. https://doi.org/10.1016/j.fct.2013.06.051
    Orlikova, Barbora ; Schumacher, Marc ; Juncker, Tom ; Yan, Choo Chee ; Inayat-Hussain, Salmaan H. ; Hajjouli, Shéhérazade ; Cerella, Claudia ; Dicato, Mario ; Diederich, Marc. / Styryl-lactone goniothalamin inhibits TNF-α-induced NF-κB activation. In: Food and Chemical Toxicology. 2013 ; Vol. 59. pp. 572-578.
    @article{82e5cbe3a0904cf88da9688265b5eb1e,
    title = "Styryl-lactone goniothalamin inhibits TNF-α-induced NF-κB activation",
    abstract = "(R)-(+)-Goniothalamin (GTN), a styryl-lactone isolated from the medicinal plant Goniothalamus macrophyllus, exhibits pharmacological activities including cytotoxic and anti-inflammatory effects. In this study, GTN modulated TNF-α induced NF-κB activation. GTN concentrations up to 20. μM showed low cytotoxic effects in K562 chronic myelogenous leukemia and in Jurkat T cells. Importantly, at these concentrations, no cytotoxicity was observed in healthy peripheral blood mononuclear cells. Our results confirmed that GTN inhibited tumor necrosis factor-α (TNF-α)-induced NF-κB activation in Jurkat and K562 leukemia cells at concentrations as low as 5. μM as shown by reporter gene assays and western blots. Moreover, GTN down-regulated translocation of the p50/p65 heterodimer to the nucleus, prevented binding of NF-κB to its DNA response element and reduced TNF-α-activated interleukin-8 (IL-8) expression. In conclusion, GTN inhibits TNF-α-induced NF-κB activation at non-apoptogenic concentrations in different leukemia cell models without presenting toxicity towards healthy blood cells underlining the anti-leukemic potential of this natural compound.",
    keywords = "Anti-cancer drug discovery, Cancer, Inflammation, NF-κB, Plant natural product",
    author = "Barbora Orlikova and Marc Schumacher and Tom Juncker and Yan, {Choo Chee} and Inayat-Hussain, {Salmaan H.} and Sh{\'e}h{\'e}razade Hajjouli and Claudia Cerella and Mario Dicato and Marc Diederich",
    year = "2013",
    month = "9",
    doi = "10.1016/j.fct.2013.06.051",
    language = "English",
    volume = "59",
    pages = "572--578",
    journal = "Food and Chemical Toxicology",
    issn = "0278-6915",
    publisher = "Elsevier Limited",

    }

    TY - JOUR

    T1 - Styryl-lactone goniothalamin inhibits TNF-α-induced NF-κB activation

    AU - Orlikova, Barbora

    AU - Schumacher, Marc

    AU - Juncker, Tom

    AU - Yan, Choo Chee

    AU - Inayat-Hussain, Salmaan H.

    AU - Hajjouli, Shéhérazade

    AU - Cerella, Claudia

    AU - Dicato, Mario

    AU - Diederich, Marc

    PY - 2013/9

    Y1 - 2013/9

    N2 - (R)-(+)-Goniothalamin (GTN), a styryl-lactone isolated from the medicinal plant Goniothalamus macrophyllus, exhibits pharmacological activities including cytotoxic and anti-inflammatory effects. In this study, GTN modulated TNF-α induced NF-κB activation. GTN concentrations up to 20. μM showed low cytotoxic effects in K562 chronic myelogenous leukemia and in Jurkat T cells. Importantly, at these concentrations, no cytotoxicity was observed in healthy peripheral blood mononuclear cells. Our results confirmed that GTN inhibited tumor necrosis factor-α (TNF-α)-induced NF-κB activation in Jurkat and K562 leukemia cells at concentrations as low as 5. μM as shown by reporter gene assays and western blots. Moreover, GTN down-regulated translocation of the p50/p65 heterodimer to the nucleus, prevented binding of NF-κB to its DNA response element and reduced TNF-α-activated interleukin-8 (IL-8) expression. In conclusion, GTN inhibits TNF-α-induced NF-κB activation at non-apoptogenic concentrations in different leukemia cell models without presenting toxicity towards healthy blood cells underlining the anti-leukemic potential of this natural compound.

    AB - (R)-(+)-Goniothalamin (GTN), a styryl-lactone isolated from the medicinal plant Goniothalamus macrophyllus, exhibits pharmacological activities including cytotoxic and anti-inflammatory effects. In this study, GTN modulated TNF-α induced NF-κB activation. GTN concentrations up to 20. μM showed low cytotoxic effects in K562 chronic myelogenous leukemia and in Jurkat T cells. Importantly, at these concentrations, no cytotoxicity was observed in healthy peripheral blood mononuclear cells. Our results confirmed that GTN inhibited tumor necrosis factor-α (TNF-α)-induced NF-κB activation in Jurkat and K562 leukemia cells at concentrations as low as 5. μM as shown by reporter gene assays and western blots. Moreover, GTN down-regulated translocation of the p50/p65 heterodimer to the nucleus, prevented binding of NF-κB to its DNA response element and reduced TNF-α-activated interleukin-8 (IL-8) expression. In conclusion, GTN inhibits TNF-α-induced NF-κB activation at non-apoptogenic concentrations in different leukemia cell models without presenting toxicity towards healthy blood cells underlining the anti-leukemic potential of this natural compound.

    KW - Anti-cancer drug discovery

    KW - Cancer

    KW - Inflammation

    KW - NF-κB

    KW - Plant natural product

    UR - http://www.scopus.com/inward/record.url?scp=84881002603&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84881002603&partnerID=8YFLogxK

    U2 - 10.1016/j.fct.2013.06.051

    DO - 10.1016/j.fct.2013.06.051

    M3 - Article

    VL - 59

    SP - 572

    EP - 578

    JO - Food and Chemical Toxicology

    JF - Food and Chemical Toxicology

    SN - 0278-6915

    ER -