Study on waxy gene polymorphism and amylose content of breeding lines derived from oryza rufipogon x oryza sativa CV. MR219

Y. Ong, M. S. Ngu, F. Parviz, Tilakavati Karupaiah, R Wickneswari V Ratnam

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Amylose in rice is controlled by granule-bound starch synthase, a protein encoded by the Waxy (Wx) gene. This study was conducted to identify the polymorphisms in the partial Waxy gene for two BC2F7 and nine BC4F3, breeding lines and their parents, Oryza sativa cv. MR219 and O. rufipogon, and also the association between the polymorphism of the partial Waxy gene and the amylose content. Sequences of all 13 breeding lines showed that the microsatellite (CT)17 located 55bp upstream of the 5'-leader intron splice site and had G at the first nucleotide of the splice donor site of intron 1 of the Wx gene. The amylose content analysis revealed that all the samples with similar (CT)17 were in the intermediate category (20-25%), except for one BC2F7 line (19%). The genotype determined using the microsatelites and SNP markers supports the intermediate category of the amylose content values.

Original languageEnglish
Pages (from-to)833-842
Number of pages10
JournalPertanika Journal of Tropical Agricultural Science
Volume35
Issue number4
Publication statusPublished - Nov 2012

Fingerprint

Oryza rufipogon
breeding lines
amylose
Oryza sativa
genetic polymorphism
introns
genes
starch synthase
granules
nucleotides
microsatellite repeats
rice
genotype
proteins
sampling

Keywords

  • Amylose content
  • Oryza rufipogon
  • Waxy gene

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

@article{0ef2b8aad2b2432b9bfc4b985289f621,
title = "Study on waxy gene polymorphism and amylose content of breeding lines derived from oryza rufipogon x oryza sativa CV. MR219",
abstract = "Amylose in rice is controlled by granule-bound starch synthase, a protein encoded by the Waxy (Wx) gene. This study was conducted to identify the polymorphisms in the partial Waxy gene for two BC2F7 and nine BC4F3, breeding lines and their parents, Oryza sativa cv. MR219 and O. rufipogon, and also the association between the polymorphism of the partial Waxy gene and the amylose content. Sequences of all 13 breeding lines showed that the microsatellite (CT)17 located 55bp upstream of the 5'-leader intron splice site and had G at the first nucleotide of the splice donor site of intron 1 of the Wx gene. The amylose content analysis revealed that all the samples with similar (CT)17 were in the intermediate category (20-25{\%}), except for one BC2F7 line (19{\%}). The genotype determined using the microsatelites and SNP markers supports the intermediate category of the amylose content values.",
keywords = "Amylose content, Oryza rufipogon, Waxy gene",
author = "Y. Ong and Ngu, {M. S.} and F. Parviz and Tilakavati Karupaiah and {V Ratnam}, {R Wickneswari}",
year = "2012",
month = "11",
language = "English",
volume = "35",
pages = "833--842",
journal = "Pertanika Journal of Tropical Agricultural Science",
issn = "1511-3701",
publisher = "Universiti Putra Malaysia",
number = "4",

}

TY - JOUR

T1 - Study on waxy gene polymorphism and amylose content of breeding lines derived from oryza rufipogon x oryza sativa CV. MR219

AU - Ong, Y.

AU - Ngu, M. S.

AU - Parviz, F.

AU - Karupaiah, Tilakavati

AU - V Ratnam, R Wickneswari

PY - 2012/11

Y1 - 2012/11

N2 - Amylose in rice is controlled by granule-bound starch synthase, a protein encoded by the Waxy (Wx) gene. This study was conducted to identify the polymorphisms in the partial Waxy gene for two BC2F7 and nine BC4F3, breeding lines and their parents, Oryza sativa cv. MR219 and O. rufipogon, and also the association between the polymorphism of the partial Waxy gene and the amylose content. Sequences of all 13 breeding lines showed that the microsatellite (CT)17 located 55bp upstream of the 5'-leader intron splice site and had G at the first nucleotide of the splice donor site of intron 1 of the Wx gene. The amylose content analysis revealed that all the samples with similar (CT)17 were in the intermediate category (20-25%), except for one BC2F7 line (19%). The genotype determined using the microsatelites and SNP markers supports the intermediate category of the amylose content values.

AB - Amylose in rice is controlled by granule-bound starch synthase, a protein encoded by the Waxy (Wx) gene. This study was conducted to identify the polymorphisms in the partial Waxy gene for two BC2F7 and nine BC4F3, breeding lines and their parents, Oryza sativa cv. MR219 and O. rufipogon, and also the association between the polymorphism of the partial Waxy gene and the amylose content. Sequences of all 13 breeding lines showed that the microsatellite (CT)17 located 55bp upstream of the 5'-leader intron splice site and had G at the first nucleotide of the splice donor site of intron 1 of the Wx gene. The amylose content analysis revealed that all the samples with similar (CT)17 were in the intermediate category (20-25%), except for one BC2F7 line (19%). The genotype determined using the microsatelites and SNP markers supports the intermediate category of the amylose content values.

KW - Amylose content

KW - Oryza rufipogon

KW - Waxy gene

UR - http://www.scopus.com/inward/record.url?scp=84874187059&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874187059&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84874187059

VL - 35

SP - 833

EP - 842

JO - Pertanika Journal of Tropical Agricultural Science

JF - Pertanika Journal of Tropical Agricultural Science

SN - 1511-3701

IS - 4

ER -