Study on Streptococcus agalactiae infection in Javanese medaka (Oryzias javanicus Bleeker, 1854) model

Mohammad Noor Azmai Amal, Ahmad Ismail, Mohd Zamri Saad, Ina Salwany Md Yasin, Nasruddin Nurrul Shaqinah, Siti Suhaiba Mastor, Muhammad Hazim Abdul Rahman, Nurliyana Mohamad

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

This study determines the median lethal dose, and describes the clinico-pathological changes and disease development following Streptococcus agalactiae infection in Javanese medaka model. Javanese medakas were infected with S. agalactiae via intraperitoneal (IP) from 10 4 to 10 8 CFU/mL, and immersion (IM) route from 10 3 to 10 7 CFU/mL. The LD 50–240h and clinico-pathological changes of the fish was determined until 240 h post infection (hpi). Next, the disease development was determined for 96 hpi in the fish following IP and IM infection at 10 3 CFU/mL and 10 4 CFU/mL, respectively. The LD 50–240h of S. agalactiae in Javanese medaka was lower following IP injection (4.5 × 10 2 CFU/mL), compared to IM route (3.5 × 10 3 CFU/mL). The clinical signs included separating from the schooling group, swimming at the surface of water column, lethargy, erratic swimming pattern, corneal opacity and exophthalmia. Histopathological examinations revealed generalized congestion in almost all internal organs, particularly in liver and brain, while the kidney displayed tubular necrosis. Both IP and IM routes showed significant positive correlation (p < 0.05) between the CFU/g of S. agalactiae in the fish tissue and fish deaths. Moreover, the lesions for histopathological scoring in selected organs following IP and IM challenges were also reflecting the CFU/g and fish deaths. This study indicates the capability of Javanese medaka as a model organism in study of streptococcosis development.

Original languageEnglish
Pages (from-to)47-52
Number of pages6
JournalMicrobial Pathogenesis
Volume131
DOIs
Publication statusPublished - 1 Jun 2019

Fingerprint

Oryzias
Streptococcus agalactiae
Immersion
Fishes
Infection
Corneal Opacity
Lethargy
Intraperitoneal Injections
Necrosis
Kidney
Water
Liver
Brain

Keywords

  • Javanese medaka
  • Pathogenesis
  • Streptococcus agalactiae
  • Test organism

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Study on Streptococcus agalactiae infection in Javanese medaka (Oryzias javanicus Bleeker, 1854) model. / Amal, Mohammad Noor Azmai; Ismail, Ahmad; Saad, Mohd Zamri; Md Yasin, Ina Salwany; Nurrul Shaqinah, Nasruddin; Mastor, Siti Suhaiba; Abdul Rahman, Muhammad Hazim; Mohamad, Nurliyana.

In: Microbial Pathogenesis, Vol. 131, 01.06.2019, p. 47-52.

Research output: Contribution to journalArticle

Amal, Mohammad Noor Azmai ; Ismail, Ahmad ; Saad, Mohd Zamri ; Md Yasin, Ina Salwany ; Nurrul Shaqinah, Nasruddin ; Mastor, Siti Suhaiba ; Abdul Rahman, Muhammad Hazim ; Mohamad, Nurliyana. / Study on Streptococcus agalactiae infection in Javanese medaka (Oryzias javanicus Bleeker, 1854) model. In: Microbial Pathogenesis. 2019 ; Vol. 131. pp. 47-52.
@article{63f815a9238b48808bc2a1d4f909d3d4,
title = "Study on Streptococcus agalactiae infection in Javanese medaka (Oryzias javanicus Bleeker, 1854) model",
abstract = "This study determines the median lethal dose, and describes the clinico-pathological changes and disease development following Streptococcus agalactiae infection in Javanese medaka model. Javanese medakas were infected with S. agalactiae via intraperitoneal (IP) from 10 4 to 10 8 CFU/mL, and immersion (IM) route from 10 3 to 10 7 CFU/mL. The LD 50–240h and clinico-pathological changes of the fish was determined until 240 h post infection (hpi). Next, the disease development was determined for 96 hpi in the fish following IP and IM infection at 10 3 CFU/mL and 10 4 CFU/mL, respectively. The LD 50–240h of S. agalactiae in Javanese medaka was lower following IP injection (4.5 × 10 2 CFU/mL), compared to IM route (3.5 × 10 3 CFU/mL). The clinical signs included separating from the schooling group, swimming at the surface of water column, lethargy, erratic swimming pattern, corneal opacity and exophthalmia. Histopathological examinations revealed generalized congestion in almost all internal organs, particularly in liver and brain, while the kidney displayed tubular necrosis. Both IP and IM routes showed significant positive correlation (p < 0.05) between the CFU/g of S. agalactiae in the fish tissue and fish deaths. Moreover, the lesions for histopathological scoring in selected organs following IP and IM challenges were also reflecting the CFU/g and fish deaths. This study indicates the capability of Javanese medaka as a model organism in study of streptococcosis development.",
keywords = "Javanese medaka, Pathogenesis, Streptococcus agalactiae, Test organism",
author = "Amal, {Mohammad Noor Azmai} and Ahmad Ismail and Saad, {Mohd Zamri} and {Md Yasin}, {Ina Salwany} and {Nurrul Shaqinah}, Nasruddin and Mastor, {Siti Suhaiba} and {Abdul Rahman}, {Muhammad Hazim} and Nurliyana Mohamad",
year = "2019",
month = "6",
day = "1",
doi = "10.1016/j.micpath.2019.03.034",
language = "English",
volume = "131",
pages = "47--52",
journal = "Microbial Pathogenesis",
issn = "0882-4010",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Study on Streptococcus agalactiae infection in Javanese medaka (Oryzias javanicus Bleeker, 1854) model

AU - Amal, Mohammad Noor Azmai

AU - Ismail, Ahmad

AU - Saad, Mohd Zamri

AU - Md Yasin, Ina Salwany

AU - Nurrul Shaqinah, Nasruddin

AU - Mastor, Siti Suhaiba

AU - Abdul Rahman, Muhammad Hazim

AU - Mohamad, Nurliyana

PY - 2019/6/1

Y1 - 2019/6/1

N2 - This study determines the median lethal dose, and describes the clinico-pathological changes and disease development following Streptococcus agalactiae infection in Javanese medaka model. Javanese medakas were infected with S. agalactiae via intraperitoneal (IP) from 10 4 to 10 8 CFU/mL, and immersion (IM) route from 10 3 to 10 7 CFU/mL. The LD 50–240h and clinico-pathological changes of the fish was determined until 240 h post infection (hpi). Next, the disease development was determined for 96 hpi in the fish following IP and IM infection at 10 3 CFU/mL and 10 4 CFU/mL, respectively. The LD 50–240h of S. agalactiae in Javanese medaka was lower following IP injection (4.5 × 10 2 CFU/mL), compared to IM route (3.5 × 10 3 CFU/mL). The clinical signs included separating from the schooling group, swimming at the surface of water column, lethargy, erratic swimming pattern, corneal opacity and exophthalmia. Histopathological examinations revealed generalized congestion in almost all internal organs, particularly in liver and brain, while the kidney displayed tubular necrosis. Both IP and IM routes showed significant positive correlation (p < 0.05) between the CFU/g of S. agalactiae in the fish tissue and fish deaths. Moreover, the lesions for histopathological scoring in selected organs following IP and IM challenges were also reflecting the CFU/g and fish deaths. This study indicates the capability of Javanese medaka as a model organism in study of streptococcosis development.

AB - This study determines the median lethal dose, and describes the clinico-pathological changes and disease development following Streptococcus agalactiae infection in Javanese medaka model. Javanese medakas were infected with S. agalactiae via intraperitoneal (IP) from 10 4 to 10 8 CFU/mL, and immersion (IM) route from 10 3 to 10 7 CFU/mL. The LD 50–240h and clinico-pathological changes of the fish was determined until 240 h post infection (hpi). Next, the disease development was determined for 96 hpi in the fish following IP and IM infection at 10 3 CFU/mL and 10 4 CFU/mL, respectively. The LD 50–240h of S. agalactiae in Javanese medaka was lower following IP injection (4.5 × 10 2 CFU/mL), compared to IM route (3.5 × 10 3 CFU/mL). The clinical signs included separating from the schooling group, swimming at the surface of water column, lethargy, erratic swimming pattern, corneal opacity and exophthalmia. Histopathological examinations revealed generalized congestion in almost all internal organs, particularly in liver and brain, while the kidney displayed tubular necrosis. Both IP and IM routes showed significant positive correlation (p < 0.05) between the CFU/g of S. agalactiae in the fish tissue and fish deaths. Moreover, the lesions for histopathological scoring in selected organs following IP and IM challenges were also reflecting the CFU/g and fish deaths. This study indicates the capability of Javanese medaka as a model organism in study of streptococcosis development.

KW - Javanese medaka

KW - Pathogenesis

KW - Streptococcus agalactiae

KW - Test organism

UR - http://www.scopus.com/inward/record.url?scp=85063618746&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85063618746&partnerID=8YFLogxK

U2 - 10.1016/j.micpath.2019.03.034

DO - 10.1016/j.micpath.2019.03.034

M3 - Article

C2 - 30940607

AN - SCOPUS:85063618746

VL - 131

SP - 47

EP - 52

JO - Microbial Pathogenesis

JF - Microbial Pathogenesis

SN - 0882-4010

ER -