Study on ABO and RhD blood grouping: Comparison between conventional tile method and a new solid phase method (intec blood grouping test kit)

Rabeya Yousuf, Siti Aisyah Abdul Ghani, Nabeelah Abdul Khalid, Leong Chooi Fun

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Introduction: ‘InTec Blood Grouping Test kit’ using solid-phase technology is a new method which may be used at outdoor blood donation site or at bed side as an alternative to the conventional tile method in view of its stability at room temperature and fulfilled the criteria as point of care test. This study aimed to compare the efficiency of this solid phase method (InTec Blood Grouping Test Kit) with the conventional tile method in determining the ABO and RhD blood group of healthy donors. Methods: A total of 760 voluntary donors who attended the Blood Bank, Penang Hospital or offsite blood donation campaigns from April to May 2014 were recruited. The ABO and RhD blood groups were determined by the conventional tile method and the solid phase method, in which the tube method was used as the gold standard. Results: For ABO blood grouping, the tile method has shown 100% concordance results with the gold standard tube method, whereas the solid-phase method only showed concordance result for 754/760 samples (99.2%). Therefore, for ABO grouping, tile method has 100% sensitivity and specificity while the solid phase method has slightly lower sensitivity of 97.7% but both with good specificity of 100%. For RhD grouping, both the tile and solid phase methods have grouped one RhD positive specimen as negative each, thus giving the sensitivity and specificity of 99.9% and 100% for both methods respectively. Conclusion: The ‘InTec Blood Grouping Test Kit’ is suitable for offsite usage because of its simplicity and user friendliness. However, further improvement in adding the internal quality control may increase the test sensitivity and validity of the test results.

Original languageEnglish
Pages (from-to)27-32
Number of pages6
JournalMalaysian Journal of Pathology
Volume40
Issue number1
Publication statusPublished - 1 Apr 2018

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Blood Grouping and Crossmatching
Hematologic Tests
Blood Group Antigens
Blood Donors
Point-of-Care Systems
Tissue Donors
Sensitivity and Specificity
Blood Banks

Keywords

  • ABO blood group
  • Blood donor
  • Conventional tile method
  • RhD blood group
  • Solid-phase method

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Study on ABO and RhD blood grouping : Comparison between conventional tile method and a new solid phase method (intec blood grouping test kit). / Yousuf, Rabeya; Abdul Ghani, Siti Aisyah; Abdul Khalid, Nabeelah; Chooi Fun, Leong.

In: Malaysian Journal of Pathology, Vol. 40, No. 1, 01.04.2018, p. 27-32.

Research output: Contribution to journalArticle

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