Structural elucidation of fucoidan from Cladosiphon okamuranus (Okinawa mozuku)

Seng Joe Lim, Wan Aida Wan Mustapha, Sonja Schiehser, Thomas Rosenau, Stefan Böhmdorfer

Research output: Contribution to journalArticle

Abstract

Fucoidan is a sulphated polysaccharide, made up mainly of L-fucose, which is found in brown seaweeds. Its chemical structure is diverse and depends on maturity, species and geographical location. The objective of this study was to elucidate the chemical structure of fucoidan from Cladosiphon okamuranus harvested in Japan. The fucoidan was subject to purification prior to monosaccharide profiling, sulphate content determination, and linkage analysis. Our results showed that Japanese Cladosiphon okamuranus fucoidan contained 70.13 ± 0.22 wt% fucose and 15.16 ± 1.17 wt% sulphate. Other minor monosaccharides found were D-xylose, D-galactose, D-mannose, D-glucose, D-arabinose, D-rhamnose and D-glucuronic acid. Linkage analysis revealed that fucopyranoside units along the backbone are linked, through α-1,3-glycosidic bonds, with fucose branching at C-2, and one sulphate group at C-4 per every three fucose units, i.e. the structure of fucoidan from Japanese Cladosiphon okamuranus is [→3)-α-fuc(1→]0.52[→3)-α-fuc-4-OSO3-(1→]0.33[→2)-α-fuc]0.14.

LanguageEnglish
Pages222-226
Number of pages5
JournalFood Chemistry
Volume272
DOIs
Publication statusPublished - 30 Jan 2019

Fingerprint

fucoidan
Ryukyu Archipelago
Fucose
fucose
Sulfates
sulfates
Monosaccharides
monosaccharides
chemical structure
Seaweed
Rhamnose
Glucuronic Acid
glucuronic acid
Arabinose
Phaeophyceae
rhamnose
Xylose
Geographical Locations
arabinose
Mannose

Keywords

  • Brown seaweed
  • Cladosiphon okamuranus
  • Fucoidan
  • Fucoidan (PubChem CID: 92023653)
  • Linkage analysis
  • Monosaccharide analysis
  • Structural elucidation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

Cite this

Structural elucidation of fucoidan from Cladosiphon okamuranus (Okinawa mozuku). / Lim, Seng Joe; Wan Mustapha, Wan Aida; Schiehser, Sonja; Rosenau, Thomas; Böhmdorfer, Stefan.

In: Food Chemistry, Vol. 272, 30.01.2019, p. 222-226.

Research output: Contribution to journalArticle

Lim, Seng Joe ; Wan Mustapha, Wan Aida ; Schiehser, Sonja ; Rosenau, Thomas ; Böhmdorfer, Stefan. / Structural elucidation of fucoidan from Cladosiphon okamuranus (Okinawa mozuku). In: Food Chemistry. 2019 ; Vol. 272. pp. 222-226.
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