Streptomyces monashensis sp. nov., a novel mangrove soil actinobacterium from East Malaysia with antioxidative potential

Jodi Woan Fei Law, Hooi Leng Ser, Nurul Syakima Ab Mutalib, Surasak Saokaew, Acharaporn Duangjai, Tahir Mehmood Khan, Kok Gan Chan, Bey Hing Goh, Learn Han Lee

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Abstract

A new Streptomyces species discovered from Sarawak mangrove soil is described, with the proposed name – Streptomyces monashensis sp. nov. (strain MUSC 1J T ). Taxonomy status of MUSC 1J T was determined via polyphasic approach. Phylogenetic and chemotaxonomic properties of strain MUSC 1J T were in accordance with those known for genus Streptomyces. Based on phylogenetic analyses, the strains closely related to MUSC 1J T were Streptomyces corchorusii DSM 40340 T (98.7%), Streptomyces olivaceoviridis NBRC 13066 T (98.7%), Streptomyces canarius NBRC 13431 T (98.6%) and Streptomyces coacervatus AS-0823 T (98.4%). Outcomes of DNA–DNA relatedness between strain MUSC 1J T and its closely related type strains covered from 19.7 ± 2.8% to 49.1 ± 4.3%. Strain MUSC 1J T has genome size of 10,254,857 bp with DNA G + C content of 71 mol%. MUSC 1J T extract exhibited strong antioxidative activity up to 83.80 ± 4.80% in the SOD assay, with significant cytotoxic effect against colon cancer cell lines HCT-116 and SW480. Streptomyces monashensis MUSC 1J T (=DSM 103626 T = MCCC 1K03221 T ) could potentially be a producer of novel bioactive metabolites; hence discovery of this new species may be highly significant to the biopharmaceutical industry as it could lead to development of new and useful chemo-preventive drugs.

Original languageEnglish
Article number3056
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - 1 Dec 2019

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Actinobacteria
Malaysia
Streptomyces
Soil
Genome Size
Base Composition
Colonic Neoplasms
Names
Industry
Cell Line
DNA
Pharmaceutical Preparations

ASJC Scopus subject areas

  • General

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Streptomyces monashensis sp. nov., a novel mangrove soil actinobacterium from East Malaysia with antioxidative potential. / Law, Jodi Woan Fei; Ser, Hooi Leng; Ab Mutalib, Nurul Syakima; Saokaew, Surasak; Duangjai, Acharaporn; Khan, Tahir Mehmood; Chan, Kok Gan; Goh, Bey Hing; Lee, Learn Han.

In: Scientific Reports, Vol. 9, No. 1, 3056, 01.12.2019.

Research output: Contribution to journalArticle

Law, Jodi Woan Fei ; Ser, Hooi Leng ; Ab Mutalib, Nurul Syakima ; Saokaew, Surasak ; Duangjai, Acharaporn ; Khan, Tahir Mehmood ; Chan, Kok Gan ; Goh, Bey Hing ; Lee, Learn Han. / Streptomyces monashensis sp. nov., a novel mangrove soil actinobacterium from East Malaysia with antioxidative potential. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
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abstract = "A new Streptomyces species discovered from Sarawak mangrove soil is described, with the proposed name – Streptomyces monashensis sp. nov. (strain MUSC 1J T ). Taxonomy status of MUSC 1J T was determined via polyphasic approach. Phylogenetic and chemotaxonomic properties of strain MUSC 1J T were in accordance with those known for genus Streptomyces. Based on phylogenetic analyses, the strains closely related to MUSC 1J T were Streptomyces corchorusii DSM 40340 T (98.7{\%}), Streptomyces olivaceoviridis NBRC 13066 T (98.7{\%}), Streptomyces canarius NBRC 13431 T (98.6{\%}) and Streptomyces coacervatus AS-0823 T (98.4{\%}). Outcomes of DNA–DNA relatedness between strain MUSC 1J T and its closely related type strains covered from 19.7 ± 2.8{\%} to 49.1 ± 4.3{\%}. Strain MUSC 1J T has genome size of 10,254,857 bp with DNA G + C content of 71 mol{\%}. MUSC 1J T extract exhibited strong antioxidative activity up to 83.80 ± 4.80{\%} in the SOD assay, with significant cytotoxic effect against colon cancer cell lines HCT-116 and SW480. Streptomyces monashensis MUSC 1J T (=DSM 103626 T = MCCC 1K03221 T ) could potentially be a producer of novel bioactive metabolites; hence discovery of this new species may be highly significant to the biopharmaceutical industry as it could lead to development of new and useful chemo-preventive drugs.",
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