Streptomyces kebangsaanensis sp. nov., an endophytic actinomycete isolated from an ethnomedicinal plant, which produces phenazine-1-carboxylic acid

Nurul' Izzah Mohd Sarmin, Geok Yuan Annie Tan, Christopher M M Franco, RuAngelie Edrada-Ebel, Jalifah Latip, Noraziah Mohamad Zin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A spore-forming streptomycete designated strain SUK12T was isolated from a Malaysian ethnomedicinal plant. Its taxonomic position, established using a polyphasic approach, indicates that it is a novel species of the genus Streptomyces. Morphological and chemical characteristics of the strain were consistent with those of members of the genus Streptomyces. Analysis of the almost complete 16S rRNA gene sequence placed strain SUK12T in the genus Streptomyces where it formed a distinct phyletic line with recognized species of this genus. The strain exhibited highest sequence similarity to Streptomyces corchorusii DSM 40340T (98.2 %) followed by Streptomyces chrestomyceticus NRRL B-3310T (98.1 %). The G+C content of the genomic DNA was 74 mol%. Chemotaxonomic data [MK-9(H8) as the major menaquinone; LLdiaminopimelic acid as a component of cell-wall peptidoglycan; C12: 0, C14: 0, C15: 0 and C17: 1 as the major fatty acids; phospholipid type II] supported the affiliation of strain SUK12T to the genus Streptomyces. The results of the phylogenetic analysis and phenotypic data derived from this and previous studies allowed the genotypic and phenotypic differentiation of strain SUK12T from the related species of the genus Streptomyces. The DNA-DNA relatedness value between strain SUK12T and S. corchorusii DSM 40340T is 18.85±4.55 %. Strain SUK12T produces phenazine-1-carboxylic acid, known as tubermycin B, an antibacterial agent. It is proposed, therefore, that strain SUK12T (=DSM 42048T=NRRL B-24860T) be classified in the genus Streptomyces as the type strain of Streptomyces kebangsaanensis sp. nov.

Original languageEnglish
Pages (from-to)3733-3738
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume63
Issue numberPART10
DOIs
Publication statusPublished - Oct 2013

Fingerprint

phenazines
Actinobacteria
Streptomyces
carboxylic acid
carboxylic acids
DNA
new species
Streptomyces corchorusii
phospholipid
relatedness
spore
genomics
fatty acid
phylogenetics
Streptomyces chrestomyceticus
gene
acid
1-phenazinecarboxylic acid
Vitamin K 2
Peptidoglycan

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Streptomyces kebangsaanensis sp. nov., an endophytic actinomycete isolated from an ethnomedicinal plant, which produces phenazine-1-carboxylic acid. / Sarmin, Nurul' Izzah Mohd; Tan, Geok Yuan Annie; Franco, Christopher M M; Edrada-Ebel, RuAngelie; Latip, Jalifah; Mohamad Zin, Noraziah.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 63, No. PART10, 10.2013, p. 3733-3738.

Research output: Contribution to journalArticle

@article{ce6a46a40d204073b7846e5ced4b4199,
title = "Streptomyces kebangsaanensis sp. nov., an endophytic actinomycete isolated from an ethnomedicinal plant, which produces phenazine-1-carboxylic acid",
abstract = "A spore-forming streptomycete designated strain SUK12T was isolated from a Malaysian ethnomedicinal plant. Its taxonomic position, established using a polyphasic approach, indicates that it is a novel species of the genus Streptomyces. Morphological and chemical characteristics of the strain were consistent with those of members of the genus Streptomyces. Analysis of the almost complete 16S rRNA gene sequence placed strain SUK12T in the genus Streptomyces where it formed a distinct phyletic line with recognized species of this genus. The strain exhibited highest sequence similarity to Streptomyces corchorusii DSM 40340T (98.2 {\%}) followed by Streptomyces chrestomyceticus NRRL B-3310T (98.1 {\%}). The G+C content of the genomic DNA was 74 mol{\%}. Chemotaxonomic data [MK-9(H8) as the major menaquinone; LLdiaminopimelic acid as a component of cell-wall peptidoglycan; C12: 0, C14: 0, C15: 0 and C17: 1 as the major fatty acids; phospholipid type II] supported the affiliation of strain SUK12T to the genus Streptomyces. The results of the phylogenetic analysis and phenotypic data derived from this and previous studies allowed the genotypic and phenotypic differentiation of strain SUK12T from the related species of the genus Streptomyces. The DNA-DNA relatedness value between strain SUK12T and S. corchorusii DSM 40340T is 18.85±4.55 {\%}. Strain SUK12T produces phenazine-1-carboxylic acid, known as tubermycin B, an antibacterial agent. It is proposed, therefore, that strain SUK12T (=DSM 42048T=NRRL B-24860T) be classified in the genus Streptomyces as the type strain of Streptomyces kebangsaanensis sp. nov.",
author = "Sarmin, {Nurul' Izzah Mohd} and Tan, {Geok Yuan Annie} and Franco, {Christopher M M} and RuAngelie Edrada-Ebel and Jalifah Latip and {Mohamad Zin}, Noraziah",
year = "2013",
month = "10",
doi = "10.1099/ijs.0.047878-0",
language = "English",
volume = "63",
pages = "3733--3738",
journal = "International Journal of Systematic and Evolutionary Microbiology",
issn = "1466-5026",
publisher = "Society for General Microbiology",
number = "PART10",

}

TY - JOUR

T1 - Streptomyces kebangsaanensis sp. nov., an endophytic actinomycete isolated from an ethnomedicinal plant, which produces phenazine-1-carboxylic acid

AU - Sarmin, Nurul' Izzah Mohd

AU - Tan, Geok Yuan Annie

AU - Franco, Christopher M M

AU - Edrada-Ebel, RuAngelie

AU - Latip, Jalifah

AU - Mohamad Zin, Noraziah

PY - 2013/10

Y1 - 2013/10

N2 - A spore-forming streptomycete designated strain SUK12T was isolated from a Malaysian ethnomedicinal plant. Its taxonomic position, established using a polyphasic approach, indicates that it is a novel species of the genus Streptomyces. Morphological and chemical characteristics of the strain were consistent with those of members of the genus Streptomyces. Analysis of the almost complete 16S rRNA gene sequence placed strain SUK12T in the genus Streptomyces where it formed a distinct phyletic line with recognized species of this genus. The strain exhibited highest sequence similarity to Streptomyces corchorusii DSM 40340T (98.2 %) followed by Streptomyces chrestomyceticus NRRL B-3310T (98.1 %). The G+C content of the genomic DNA was 74 mol%. Chemotaxonomic data [MK-9(H8) as the major menaquinone; LLdiaminopimelic acid as a component of cell-wall peptidoglycan; C12: 0, C14: 0, C15: 0 and C17: 1 as the major fatty acids; phospholipid type II] supported the affiliation of strain SUK12T to the genus Streptomyces. The results of the phylogenetic analysis and phenotypic data derived from this and previous studies allowed the genotypic and phenotypic differentiation of strain SUK12T from the related species of the genus Streptomyces. The DNA-DNA relatedness value between strain SUK12T and S. corchorusii DSM 40340T is 18.85±4.55 %. Strain SUK12T produces phenazine-1-carboxylic acid, known as tubermycin B, an antibacterial agent. It is proposed, therefore, that strain SUK12T (=DSM 42048T=NRRL B-24860T) be classified in the genus Streptomyces as the type strain of Streptomyces kebangsaanensis sp. nov.

AB - A spore-forming streptomycete designated strain SUK12T was isolated from a Malaysian ethnomedicinal plant. Its taxonomic position, established using a polyphasic approach, indicates that it is a novel species of the genus Streptomyces. Morphological and chemical characteristics of the strain were consistent with those of members of the genus Streptomyces. Analysis of the almost complete 16S rRNA gene sequence placed strain SUK12T in the genus Streptomyces where it formed a distinct phyletic line with recognized species of this genus. The strain exhibited highest sequence similarity to Streptomyces corchorusii DSM 40340T (98.2 %) followed by Streptomyces chrestomyceticus NRRL B-3310T (98.1 %). The G+C content of the genomic DNA was 74 mol%. Chemotaxonomic data [MK-9(H8) as the major menaquinone; LLdiaminopimelic acid as a component of cell-wall peptidoglycan; C12: 0, C14: 0, C15: 0 and C17: 1 as the major fatty acids; phospholipid type II] supported the affiliation of strain SUK12T to the genus Streptomyces. The results of the phylogenetic analysis and phenotypic data derived from this and previous studies allowed the genotypic and phenotypic differentiation of strain SUK12T from the related species of the genus Streptomyces. The DNA-DNA relatedness value between strain SUK12T and S. corchorusii DSM 40340T is 18.85±4.55 %. Strain SUK12T produces phenazine-1-carboxylic acid, known as tubermycin B, an antibacterial agent. It is proposed, therefore, that strain SUK12T (=DSM 42048T=NRRL B-24860T) be classified in the genus Streptomyces as the type strain of Streptomyces kebangsaanensis sp. nov.

UR - http://www.scopus.com/inward/record.url?scp=84885226659&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84885226659&partnerID=8YFLogxK

U2 - 10.1099/ijs.0.047878-0

DO - 10.1099/ijs.0.047878-0

M3 - Article

VL - 63

SP - 3733

EP - 3738

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

IS - PART10

ER -