Strategy for generating tissue-engineered human bone construct.

K. K. Tan, B. S. Aminuddin, G. H. Tan, Sabarul Afian Mokhtar, Min Hwei Ng, O. Fauziah, Ruszymah Idrus

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The strategy used to generate tissue-engineered bone construct, in view of future clinical application is presented here. Osteoprogenitor cells from periosteum of consenting scoliosis patients were isolated. Growth factors viz TGF-B2, bFGF and IGF-1 were used in concert to increase cell proliferation during in vitro cell expansion. Porous tricalcium phosphate (TCP)-hydroxyapatite (HA) scaffold was used as the scaffold to form 3D bone construct. We found that the addition of growth factors, greatly increased cell growth by 2 to 7 fold. TCP/HA proved to be the ideal scaffold for cell attachment and proliferation. Hence, this model will be further carried out on animal trial.

Original languageEnglish
Pages (from-to)43-44
Number of pages2
JournalThe Medical journal of Malaysia
Volume59 Suppl B
Publication statusPublished - May 2004

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Durapatite
Bone and Bones
Intercellular Signaling Peptides and Proteins
Cell Proliferation
Periosteum
Scoliosis
Insulin-Like Growth Factor I
Growth
tricalcium phosphate
In Vitro Techniques

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Strategy for generating tissue-engineered human bone construct. / Tan, K. K.; Aminuddin, B. S.; Tan, G. H.; Mokhtar, Sabarul Afian; Ng, Min Hwei; Fauziah, O.; Idrus, Ruszymah.

In: The Medical journal of Malaysia, Vol. 59 Suppl B, 05.2004, p. 43-44.

Research output: Contribution to journalArticle

Tan KK, Aminuddin BS, Tan GH, Mokhtar SA, Ng MH, Fauziah O et al. Strategy for generating tissue-engineered human bone construct. The Medical journal of Malaysia. 2004 May;59 Suppl B:43-44.
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