Abstract
The strategy used to generate tissue-engineered bone construct, in view of future clinical application is presented here. Osteoprogenitor cells from periosteum of consenting scoliosis patients were isolated. Growth factors viz TGF-B2, bFGF and IGF-1 were used in concert to increase cell proliferation during in vitro cell expansion. Porous tricalcium phosphate (TCP)-hydroxyapatite (HA) scaffold was used as the scaffold to form 3D bone construct. We found that the addition of growth factors, greatly increased cell growth by 2 to 7 fold. TCP/HA proved to be the ideal scaffold for cell attachment and proliferation. Hence, this model will be further carried out on animal trial.
| Original language | English |
|---|---|
| Pages (from-to) | 43-44 |
| Number of pages | 2 |
| Journal | The Medical journal of Malaysia |
| Volume | 59 Suppl B |
| Publication status | Published - May 2004 |
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ASJC Scopus subject areas
- Medicine(all)
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Strategy for generating tissue-engineered human bone construct. / Tan, K. K.; Aminuddin, B. S.; Tan, G. H.; Mokhtar, Sabarul Afian; Ng, Min Hwei; Fauziah, O.; Idrus, Ruszymah.
In: The Medical journal of Malaysia, Vol. 59 Suppl B, 05.2004, p. 43-44.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Strategy for generating tissue-engineered human bone construct.
AU - Tan, K. K.
AU - Aminuddin, B. S.
AU - Tan, G. H.
AU - Mokhtar, Sabarul Afian
AU - Ng, Min Hwei
AU - Fauziah, O.
AU - Idrus, Ruszymah
PY - 2004/5
Y1 - 2004/5
N2 - The strategy used to generate tissue-engineered bone construct, in view of future clinical application is presented here. Osteoprogenitor cells from periosteum of consenting scoliosis patients were isolated. Growth factors viz TGF-B2, bFGF and IGF-1 were used in concert to increase cell proliferation during in vitro cell expansion. Porous tricalcium phosphate (TCP)-hydroxyapatite (HA) scaffold was used as the scaffold to form 3D bone construct. We found that the addition of growth factors, greatly increased cell growth by 2 to 7 fold. TCP/HA proved to be the ideal scaffold for cell attachment and proliferation. Hence, this model will be further carried out on animal trial.
AB - The strategy used to generate tissue-engineered bone construct, in view of future clinical application is presented here. Osteoprogenitor cells from periosteum of consenting scoliosis patients were isolated. Growth factors viz TGF-B2, bFGF and IGF-1 were used in concert to increase cell proliferation during in vitro cell expansion. Porous tricalcium phosphate (TCP)-hydroxyapatite (HA) scaffold was used as the scaffold to form 3D bone construct. We found that the addition of growth factors, greatly increased cell growth by 2 to 7 fold. TCP/HA proved to be the ideal scaffold for cell attachment and proliferation. Hence, this model will be further carried out on animal trial.
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UR - http://www.scopus.com/inward/citedby.url?scp=16544362172&partnerID=8YFLogxK
M3 - Article
C2 - 15468810
AN - SCOPUS:16544362172
VL - 59 Suppl B
SP - 43
EP - 44
JO - Medical Journal of Malaysia
JF - Medical Journal of Malaysia
SN - 0300-5283
ER -